scholarly journals Xanthohumol-supplemented beer modulates angiogenesis and inflammation in a skin wound healing model. Involvement of local adipocytes

2011 ◽  
Vol 113 (1) ◽  
pp. 100-109 ◽  
Author(s):  
Rita Negrão ◽  
Raquel Costa ◽  
Delfim Duarte ◽  
Tiago Taveira Gomes ◽  
Pedro Coelho ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Wound Healing Model ◽  
Healing Model

Interleukin-1 Beta Regulates Lysophosphatidic Acid-accelerated Skin Wound Healing

2020 ◽  
Author(s):  
Delong Ge ◽  
Xiaoxiao Mou ◽  
Liying Liu ◽  
Yanwei Li ◽  
Hui Jiang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Interleukin 1 ◽  
Skin Wound ◽  
Inflammatory Factor ◽  
Interleukin 1 Beta ◽  
Skin Wound Healing ◽  
Wound Healing Model ◽  
Kinetics Of ◽  
Healing Model

Abstract Background: Interleukin 1 beta (IL-1β) is considered to be a mediator of infectious, inflammatory and autoimmune diseases, and the kinetics of its production is relevant to understanding the pathogenesis of these diseases. Lysophosphatidic acid (LPA), the structurally simplest bioactive phospholipid, is necessary for homeostasis in various physiological and pathophysiological processes and plays a pivotal role in wound healing. Skin trauma can not only weaken the barrier function, but also cause pain and infection. Chronic wounds are characterized by impaired healing and uncontrolled inflammation that damages the protection of the immune system. The aim of this study is to investigate whether inflammatory factor IL-1β has an effect on LPA in the wound healing model. Results: In this study, the kinetics of IL-1β gene expression was studied in vivo and in vitro with a wound healing model by quantitative real-time polymerase chain reaction (qRT-PCR) through LPA treatment. As a result, we found that LPA up-regulated inflammatory factor IL-1β in HaCaT cell and skin wound healing. The pro-inflammatory cytokines IL-1β mRNA had higher expression in LPA-treated mice group 3 days after the treatment. In vitro, after the treatment with LPA (20 μM) for 6, 12, and 24 hours, IL-1β mRNA expression increased by 61.16%, 129.39%, and 117.07%, respectively. Conclusion: These results strongly suggest that IL-1β may regulate LPA-accelerated skin wound healing. IL-1β has significant efficacy, and our observations are of interest to the development of drugs targeting LPA in skin therapy.

Skin Wound Healing Model - Excisional Wounding and Assessment of Lesion Area

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (22) ◽  
Author(s):  
Camila Moreira ◽  
Puebla Cassini-Vieira ◽  
Mariana da Silva ◽  
Luc�ola da Barcelos
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Lesion Area ◽  
Skin Wound Healing ◽  
Wound Healing Model ◽  
Healing Model

Characterization of a novel standardized human three‐dimensional skin wound healing model using non‐sequential fractional ultrapulsed CO 2 laser treatments

2015 ◽  
Vol 47 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Yvonne Marquardt ◽  
Philipp M. Amann ◽  
Ruth Heise ◽  
Katharina Czaja ◽  
Timm Steiner ◽  
...  
Keyword(s):  
Wound Healing ◽  
Three Dimensional ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Laser Treatments ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1561
Author(s):  
Liubov Safonova ◽  
Maria Bobrova ◽  
Anton Efimov ◽  
Alexey Lyundup ◽  
Olga Agapova ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Silk Fibroin ◽  
Biological Properties ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Thickness Skin ◽  
Internal Structures ◽  
Wound Healing Model ◽  
Healing Model

A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine.

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

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