Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N -Propionyl Groups

2012 ◽  
Vol 12 (5) ◽  
pp. 628-636 ◽  
Author(s):  
Zuzana Kadlecova ◽  
Sophie Nallet ◽  
David L. Hacker ◽  
Lucia Baldi ◽  
Harm-Anton Klok ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Weight ◽  
Large Scale ◽  
Transient Gene Expression

Large Scale Transient Gene Expression in Mammalian Cells

2006 ◽  
pp. 113-116
Author(s):  
E. -J. Schlaeger ◽  
K. Christensen ◽  
G. Schmid ◽  
N. Schaub ◽  
B. Wipf ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Large Scale ◽  
Transient Gene Expression ◽  
Expression In Mammalian Cells

Large-scale plasmid preparation for transient gene expression

2011 ◽  
Vol 33 (8) ◽  
pp. 1559-1564 ◽  
Author(s):  
Lu Cheng ◽  
Xiangming Sun ◽  
Xiaoping Yi ◽  
Yuanxing Zhang
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Transient Gene Expression ◽  
Plasmid Preparation

scholarly journals Expression of Recombinant Factor IX Using the Transient Gene Expression Technique

2019 ◽  
Author(s):  
Jafar Vatandoost ◽  
Mohammad Amin Azimifar
Keyword(s):  
Gene Expression ◽  
Recombinant Proteins ◽  
Large Scale ◽  
Transient Gene Expression ◽  
Factor Ix ◽  
Cell Factory ◽  
Scale Production ◽  
Hek Cells ◽  
Large Scale Production ◽  
A Cell

Background: Pilot and large-scale production of recombinant proteins require the presence of stable clones, but the process of selecting stable clones is time consuming. Moreover, continuous clone culturing in large-scale production may cause loss of incoming plasmid and recombinant genes. Considering the advancements in Transient Gene Expression (TGE) technology, the large-scale expression of factor IX (FIX) was investigated in HEK cells by the TGE technique. Materials and Methods: HEK cells were seeded in a cell factory, and then transfected by pcDNA-hFIX plasmid using calcium phosphate co-precipitation method. Stable HEKhFIX cells were also seeded in a cell factory, separately. After adding vitamin K, recombinant FIX was quantified in conditioned media using an ELISA. Moreover, its functional activity was assayed using an aPTT test. Results: The results showed that the expression and activity of FIX by TGE technology was, respectively, 1.6 and 1.5 times higher than that obtained through stable HEK-FIX cells. Since calculating the specific activity revealed that for all time periods it is 0.2 mU/ng, so the increase in activity is due to the increase in the amount of FIX. Conclusions: HEK cells with higher transfectability seemed to be an appropriate alternative for transient expression for large-scale protein production. Furthermore, if rapid expression of recombinant proteins is intended, TGE can replace costly and lowyield methods.

Transient Gene Expression in Suspension HEK-293 Cells: Application to Large-Scale Protein Production

2008 ◽  
Vol 21 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Lucia Baldi ◽  
Natalie Muller ◽  
Sylviane Picasso ◽  
Raymond Jacquet ◽  
Philippe Girard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Protein Production ◽  
Transient Gene Expression ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

scholarly journals Efficient transient gene expression system using buckwheat hypocotyl protoplasts for large-scale experiments

2020 ◽  
Vol 70 (1) ◽  
pp. 128-134 ◽  
Author(s):  
Shingo Sakamoto ◽  
Katsuhiro Matsui ◽  
Yoshimi Oshima ◽  
Nobutaka Mitsuda
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Expression System ◽  
Transient Gene Expression ◽  
Gene Expression System
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