A Comparative Study of Cellular Uptake and Subcellular Localization of Doxorubicin Loaded in Self-Assemblies of Amphiphilic Copolymers with Pendant Dendron by MDA-MB-231 Human Breast Cancer Cells

2016 ◽  
Vol 16 (6) ◽  
pp. 882-895 ◽  
Author(s):  
Geetha Viswanathan ◽  
Yu-Hsuan Hsu ◽  
Siew Hui Voon ◽  
Toyoko Imae ◽  
Ampornphan Siriviriyanun ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Subcellular Localization ◽  
Comparative Study ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Amphiphilic Copolymers ◽  
Human Breast Cancer Cells ◽  
Human Breast

scholarly journals Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorinfin Human Breast Cancer Cells: A Diffusion Kinetics Study

2012 ◽  
Vol 2012 ◽  
pp. 1-8
Author(s):  
Ping Chen ◽  
Feng Zhang ◽  
Lei Zhang ◽  
Song-Cheng Mao ◽  
Lie Lin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Diffusion Kinetics ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Kinetics Of ◽  
Mcf 7

The kinetics of photosensitizer 2-devinyl-2-(1-methoxylethyl) chlorinf(CPD4) uptake in MCF-7 human breast cancer cells is described by a diffusion kinetics model and experimentally investigated using laser scanning confocal microscopy (LSCM). CPD4 permeated into MCF-7 cells with increasing incubation time, which was followed by its binding to cell organelles. Subcellular distribution study revealed that CPD4 was primarily localized on the mitochondria and membranes, supporting that the mode of transmembrane transport was diffusion. A kinetics model describing CPD4 passing through the plasma membrane of MCF-7 cells was proposed based on Fick's first law of diffusion. The kinetics of cellular uptake of CPD4 was studied by three-dimensional LSCM. By fitting the experimental data using the above model, important cellular uptake and distribution parameters were obtained, which are of clinical significance in photodynamic therapy.

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