Antiproliferative effects of pomegranate extract in MCF-7 breast cancer cells are associated with reduced DNA repair gene expression and induction of double strand breaks

2013 ◽  
Vol 53 (6) ◽  
pp. 458-470 ◽  
Author(s):  
Amit B. Shirode ◽  
Prasad Kovvuru ◽  
Sridar V. Chittur ◽  
Susanne M. Henning ◽  
David Heber ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Dna Repair ◽  
Breast Cancer Cells ◽  
Double Strand Breaks ◽  
Repair Gene ◽  
Antiproliferative Effects ◽  
Strand Breaks ◽  
Dna Repair Gene ◽  
Mcf 7

scholarly journals Apigenin and Hesperidin Downregulate DNA Repair Genes in MCF-7 Breast Cancer Cells and Augment Doxorubicin Toxicity

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4421
Author(s):  
Agnieszka Korga-Plewko ◽  
Monika Michalczyk ◽  
Grzegorz Adamczuk ◽  
Ewelina Humeniuk ◽  
Marta Ostrowska-Lesko ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Repair ◽  
Oxidative Damage ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cytotoxic Effect ◽  
Interaction Mechanism ◽  
Double Strand Breaks ◽  
Strand Breaks ◽  
Mcf 7

A number of studies have confirmed anti-tumor activity of flavonoids and their ability to enhance the effectiveness of classical anticancer drugs. The mechanism of this phenomenon is difficult to explain because of the ambivalent nature of these compounds. Many therapeutic properties of these compounds are attributed to their antioxidant activity; however, it is known that they can act as oxidants. The aim of this study was to assess the influence of apigenin and hesperidin on MCF-7 breast cancer cells with doxorubicin. The cytotoxic effect was determined using an MTT test and cell cycle analysis. To evaluate the possible interaction mechanism, reduced glutathione levels, as well as the DNA oxidative damage and the double strand breaks, were evaluated. Additionally, mRNA expression of genes related to DNA repair was assessed. It was demonstrated that flavonoids intensified the cytotoxic effect of doxorubicin despite flavonoids reduced oxidative damage caused by the drug. At the same time, the number of double strand breaks significantly increased and expression of tested genes was downregulated. In conclusion, both apigenin and hesperidin enhance the cytotoxic effects of doxorubicin on breast cancer cells, and this phenomenon occurs regardless of oxidative stress but is accompanied by disorders of DNA damage response mechanisms.

DNA Repair Gene Expression and Risk of Locoregional Relapse in Breast Cancer Patients

2010 ◽  
Vol 78 (2) ◽  
pp. 328-336 ◽  
Author(s):  
Romuald Le Scodan ◽  
Géraldine Cizeron-Clairac ◽  
Emmanuelle Fourme ◽  
Didier Meseure ◽  
Sophie Vacher ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Dna Repair ◽  
Cancer Patients ◽  
Breast Cancer Patients ◽  
Repair Gene ◽  
Locoregional Relapse ◽  
Dna Repair Gene

scholarly journals The Effect of a DNA Repair Gene on Cellular Invasiveness: Xrcc3 Over-Expression in Breast Cancer Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. e16394 ◽  
Author(s):  
Veronica L. Martinez-Marignac ◽  
Amélie Rodrigue ◽  
David Davidson ◽  
Martin Couillard ◽  
Ala-Eddin Al-Moustafa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Repair ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Repair Gene ◽  
Over Expression ◽  
Dna Repair Gene ◽  
Cellular Invasiveness

scholarly journals IKKβ Regulates the Repair of DNA Double-Strand Breaks Induced by Ionizing Radiation in MCF-7 Breast Cancer Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18447 ◽  
Author(s):  
Lixian Wu ◽  
Lijian Shao ◽  
Ningfei An ◽  
Junru Wang ◽  
Senthil Pazhanisamy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Mcf 7

The Expression of Dopamine Receptors Gene and their Potential Role in Targeting Breast Cancer Cells with Selective Agonist and Antagonist Drugs. Could it be the Novel Insight to Therapy?

2019 ◽  
Vol 16 (2) ◽  
pp. 184-197 ◽  
Author(s):  
Hossein Bakhtou ◽  
Asiie Olfatbakhsh ◽  
Abdolkhaegh Deezagi ◽  
Ghasem Ahangari
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Dopamine Receptors ◽  
Breast Cancer Cells ◽  
D2 Receptors ◽  
Healthy Individuals ◽  
Agonist And Antagonist ◽  
The Mean ◽  
Mcf 7

Background:Breast cancer is one of the common causes of mortality for women in Iran and other parts of the world. The substantial increasing rate of breast cancer in both developed and developing countries warns the scientists to provide more preventive steps and therapeutic measures. This study is conducted to investigate the impact of neurotransmitters (e.g., Dopamine) through their receptors and the importance of cancers via damaging immune system. It also evaluates dopamine receptors gene expression in the women with breast cancer at stages II or III and dopamine receptor D2 (DRD2) related agonist and antagonist drug effects on human breast cancer cells, including MCF-7 and SKBR-3.Methods:The patients were categorized into two groups: 30 native patients who were diagnosed with breast cancer at stages II and III, with the mean age of 44.6 years and they were reported to have the experience of a chronic stress or unpleasant life event. The second group included 30 individuals with the mean age of 39 years as the control group. In order to determine the RNA concentration in all samples, the RNA samples were extracted and cDNA was synthesized. The MCF-7 cells and SKBR-3 cells were treated with dopamine receptors agonists and antagonists. The MTT test was conducted to identify oxidative and reductive enzymes and to specify appropriate dosage at four concentrations of dopamine and Cabergoline on MCF-7 and SKBR-3 cells. Immunofluorescence staining was done by the use of a mixed dye containing acridine orange and ethidiume bromide on account of differentiating between apoptotic and necrotic cells. Flow cytometry assay was an applied method to differentiate necrotic from apoptotic cells.Results:Sixty seven and thirty three percent of the patients were related to stages II and III, respectively. About sixty three percent of the patients expressed ER, while fifty seven percent expressed PR. Thirty seven percent of the patients were identified as HER-2 positive. All types of D2-receptors were expressed in PBMC of patients with breast cancer and healthy individuals. The expression of the whole dopamine receptor subtypes (DRD2-DRD4) was carried out on MCF-7 cell line. The results of RT-PCR confirmed the expression of DRD2 on SKBR-3 cells, whereas the other types of D2- receptors did not have an expression. The remarkable differences in gene expression rates between patients and healthy individuals were revealed in the result of the Real-time PCR analysis. The over expression in DRD2 and DRD4 genes of PBMCs was observed in the patients with breast cancer at stages II and III. The great amount of apoptosis and necrosis occurred after the treatment of MCF-7 cells by Cabergoline from 25 to 100 µmolL-1 concentrations.Conclusion:This study revealed the features of dopamine receptors associated with apoptosis induction in breast cancer cells. Moreover, the use of D2-agonist based on dopamine receptors expression in various breast tumoral cells could be promising as a new insight of complementary therapy in breast cancer.

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