scholarly journals Detection of DNA damage in peripheral blood mononuclear cells from pancreatic cancer patients

2014 ◽  
Vol 54 (10) ◽  
pp. 1220-1226 ◽  
Author(s):  
Rick J. Jansen ◽  
Sharon Fonseca-Williams ◽  
William R. Bamlet ◽  
Sylvette Ayala-Peña ◽  
Ann L. Oberg ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Dna Damage ◽  
Cancer Patients ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals α-Particle-induced DNA damage tracks in peripheral blood mononuclear cells of [223Ra]RaCl2-treated prostate cancer patients

2021 ◽  
Author(s):  
S. Schumann ◽  
U. Eberlein ◽  
C. Lapa ◽  
J. Müller ◽  
S. Serfling ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cancer Patients ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Absorbed Dose ◽  
Peripheral Blood Mononuclear ◽  
Absorbed Doses ◽  
Blood Mononuclear Cells

Abstract Purpose One therapy option for prostate cancer patients with bone metastases is the use of [223Ra]RaCl2. The α-emitter 223Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand break markers. We investigated the time- and absorbed dose-dependency of the number of α-tracks in peripheral blood mononuclear cells (PBMCs) of patients undergoing their first therapy with [223Ra]RaCl2. Methods Multiple blood samples from nine prostate cancer patients were collected before and after administration of [223Ra]RaCl2, up to 4 weeks after treatment. γ-H2AX- and 53BP1-positive α-tracks were microscopically quantified in isolated and immuno-stained PBMCs. Results The absorbed doses to the blood were less than 6 mGy up to 4 h after administration and maximally 16 mGy in total. Up to 4 h after administration, the α-track frequency was significantly increased relative to baseline and correlated with the absorbed dose to the blood in the dose range < 3 mGy. In most of the late samples (24 h – 4 weeks after administration), the α-track frequency remained elevated. Conclusion The γ-H2AX+53BP1 assay is a potent method for detection of α-particle-induced DNA damages during treatment with or after accidental incorporation of radionuclides even at low absorbed doses. It may serve as a biomarker discriminating α- from β-emitters based on damage geometry.

In-depth immune profiling of peripheral blood mononuclear cells in pancreatic cancer patients

Pancreatology ◽  
2020 ◽  
Vol 20 ◽  
pp. S122
Author(s):  
E. Zwart ◽  
E. Rodriguez ◽  
J. Verhoeff ◽  
A. Affandi ◽  
L. Meijer ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Patients ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Immune Profiling

DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment

2012 ◽  
Vol 747 (1) ◽  
pp. 82-85 ◽  
Author(s):  
Larissa Ragozo Cardoso de Oliveira ◽  
Eliana Peresi ◽  
Francilene Capel Tavares ◽  
Camila Renata Corrêa ◽  
Damiana Tortolero Pierine ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Tuberculosis Treatment ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells
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