The efficacy of combining a vascularized biogenic conduit and a decellularized nerve graft in the treatment of peripheral nerve defects: An experimental study using the rat sciatic nerve defect model

Microsurgery ◽  
2021 ◽  
Author(s):  
Hisataka Takeuchi ◽  
Ryosuke Ikeguchi ◽  
Takashi Noguchi ◽  
Maki Ando ◽  
Koichi Yoshimoto ◽  
...  
Keyword(s):  
Experimental Study ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Graft ◽  
Defect Model ◽  
Rat Sciatic Nerve

Better nerve regeneration with distally based fascicular turnover flap than with conventional autologous nerve graft in a rat sciatic nerve defect model

2020 ◽  
Vol 73 (2) ◽  
pp. 214-221 ◽  
Author(s):  
Jaehoon Choi ◽  
Dong Seok Kim ◽  
Jinhan Kim ◽  
Woonhyeok Jeong ◽  
Hyoun Wook Lee ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Graft ◽  
Defect Model ◽  
Rat Sciatic Nerve ◽  
Distally Based

Functional characterization of optimized acellular peripheral nerve graft in a rat sciatic nerve injury model

2011 ◽  
Vol 33 (6) ◽  
pp. 600-608 ◽  
Author(s):  
Ryan J Nagao ◽  
Scott Lundy ◽  
Zin Z Khaing ◽  
Christine E Schmidt
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Functional Characterization ◽  
Nerve Graft ◽  
Sciatic Nerve Injury ◽  
Peripheral Nerve Graft ◽  
Rat Sciatic Nerve ◽  
Injury Model

Erratum: Doxorubicin-Immersed Skeletal Muscle Grafts Promote Peripheral Nerve Regeneration Across a 10-mm Defect in the Rat Sciatic Nerve

2019 ◽  
Vol 36 (01) ◽  
pp. e1-e2
Author(s):  
Hisataka Takeuchi ◽  
Akio Sakamoto ◽  
Ryosuke Ikeguchi ◽  
Soichi Ota ◽  
Hiroki Oda ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Rat Sciatic Nerve

scholarly journals Functional Recovery via Preregenerated Nerve Graft in Rat Sciatic Nerve Injury Model

Neurosurgery ◽  
2019 ◽  
Vol 66 (Supplement_1) ◽  
Author(s):  
Umang Khandpur ◽  
Ying Yan ◽  
Wilson Zachary Ray ◽  
Matthew R MacEwan
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Functional Recovery ◽  
Peripheral Nerve Injury ◽  
Muscle Force ◽  
Nerve Graft ◽  
Tissue Loss ◽  
Limb Amputation ◽  
Percent Recovery

Abstract INTRODUCTION Patients who have experienced major tissue loss with peripheral nerve injury (eg, limb amputation) may be offered composite tissue allotransplantation (CTA). The return of sensory ability and cosmetic component of CTAs make them an attractive alternative to prosthetic devices. Unfortunately, robust reinnervation especially over great distances remains an issue for hand allotransplants. In this study, we introduce a preregenerated nerve graft to shorten the distance and therefore time to terminal tissue reinnervation, which could improve the utility of CTAs. METHODS A total of 18 rats weighing 250 to 300 gm each were randomized into 1 of 3 groups: baseline, fresh, or preregenerated. The baseline group underwent sham surgery to obtain baseline functional data. The fresh and preregenerated groups both underwent grafting of the sciatic nerve but the preregenerated group utilized 8-wk preregenerated grafts. At postperative week 8 from distal neurorrhaphy, both groups underwent terminal functional testing via EMG and evoked muscle force. RESULTS The preregenerated group had significantly greater mean EMG (P < .05) and maximum tetanic muscle force values (P < .05) than the fresh group. Mean percent recovery in EMG for the fresh group was 21.95% compared with 81.79% in the preregenerated group. Mean percent recovery in muscle force was 9.46% and 33.15%, respectively. CONCLUSION The results of this study provide a novel approach to enhance final functional recovery after peripheral nerve injury. The current practice of constructing a nerve stump may be improved by grafting a nerve segment at the time of injury and allowing it to preregenerate into local musculature so that if a CTA is later performed, an expedited and more robust reinnervation could be accomplished.

scholarly journals Revascularization patterns of nerve allografts in a rat sciatic nerve defect model

2020 ◽  
Vol 73 (3) ◽  
pp. 460-468 ◽  
Author(s):  
Tiam M. Saffari ◽  
Femke Mathot ◽  
Patricia F. Friedrich ◽  
Allen T. Bishop ◽  
Alexander Y. Shin
Keyword(s):  
Sciatic Nerve ◽  
Defect Model ◽  
Rat Sciatic Nerve

scholarly journals Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

2018 ◽  
Vol 6 (5) ◽  
pp. 1059-1075 ◽  
Author(s):  
C. R. Carvalho ◽  
S. Wrobel ◽  
C. Meyer ◽  
C. Brandenberger ◽  
I. F. Cengiz ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Experimental Work ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Gellan Gum ◽  
In Vivo Study ◽  
Rat Sciatic Nerve

This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels.

Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft

2019 ◽  
Vol 129 ◽  
pp. 997-1005 ◽  
Author(s):  
Zhiwen Jiang ◽  
Yanan Song ◽  
Jing Qiao ◽  
Yan Yang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Graft ◽  
Rat Sciatic Nerve ◽  
Sciatic Nerve Regeneration

scholarly journals E8002 Inhibits Peripheral Nerve Adhesion by Enhancing Fibrinolysis of l-Ascorbic Acid in a Rat Sciatic Nerve Model

2020 ◽  
Vol 21 (11) ◽  
pp. 3972
Author(s):  
Kiyoshi Kikuchi ◽  
Kentaro Setoyama ◽  
Seiya Takada ◽  
Shotaro Otsuka ◽  
Kazuki Nakanishi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
In Vitro Study ◽  
Scar Tissue ◽  
Tissue Type ◽  
Rat Sciatic Nerve ◽  
Peripheral Nerve Surgery ◽  
Nerve Surgery

Perineural adhesions leading to neuropathy are one of the most undesirable consequences of peripheral nerve surgery. However, there are currently no widely used compounds with anti-adhesive effects in the field of peripheral nerve surgery. E8002 is a novel, anti-adhesive, multi-layer membrane that contains L-ascorbic acid (AA). Here, we investigated the effect and mechanism of E8002 in a rat sciatic nerve adhesion model. A total of 21 rats were used. Six weeks after surgery, macroscopic adhesion scores were significantly lower in the E8002 group (adhesion procedure followed by nerve wrapping with E8002) compared to the E8002 AA(−) group (adhesion procedure followed by nerve wrapping with the E8002 membrane excluding AA) and adhesion group (adhesion procedure but no treatment). Correspondingly, a microscopic examination revealed prominent scar tissue in the E8002 AA(−) and adhesion groups. Furthermore, an in vitro study using human blood samples showed that AA enhanced tissue-type, plasminogen activator-mediated fibrinolysis. Altogether, these results suggest that E8002 may exert an anti-adhesive action via AA and the regulation of fibrinolysis.

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