Demonstration of Epstein-Barr viral DNA in formalin-fixed, paraffin-embedded samples of Hodgkin's disease

1991 ◽  
Vol 163 (2) ◽  
pp. 149-151 ◽  
Author(s):  
Sarah Gledhill ◽  
Andrew S. Krajewski ◽  
Ruth F. Jarrett ◽  
J. Howard Pringle ◽  
Anthony Warford ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Hodgkin’S Disease ◽  
Viral Dna ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

Demonstration of Epstein-Barr viral DNA in formalin-fixed, paraffin-embedded samples of Hodgkin's disease

1990 ◽  
Vol 161 (3) ◽  
pp. 255-260 ◽  
Author(s):  
Carole M. Libetta ◽  
J. H. Pringle ◽  
Carole A. Angel ◽  
A. W. Craft ◽  
A. J. Malcolm ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Hodgkin’S Disease ◽  
Viral Dna ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

scholarly journals Frequency of Epstein–Barr Virus DNA in Formalin-fixed Paraffin-embedded Tissue of Patients with Ductal Breast Carcinoma

2019 ◽  
Vol 20 (3) ◽  
pp. 687-692 ◽  
Author(s):  
Chia Sharifpour ◽  
Manoochehr Makvandi ◽  
Alireza Samabafzadeh ◽  
Abdolhassan Talaei-Zadeh ◽  
Nastaran Ranjbari ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Ductal Breast Carcinoma ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

scholarly journals Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity

Blood ◽  
1994 ◽  
Vol 83 (6) ◽  
pp. 1595-1602 ◽  
Author(s):  
ML Gulley ◽  
PA Eagan ◽  
L Quintanilla-Martinez ◽  
AL Picado ◽  
BN Smir ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Odds Ratio ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Viral Dna ◽  
Mixed Cellularity ◽  
Hispanic Ethnicity ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

One hundred twenty-five cases of Hodgkin's disease from the United States (79), Mexico City (31), and Costa Rica (15) were analyzed for the presence of Epstein-Barr virus (EBV) by in situ hybridization to EBER1 transcripts. EBV was more frequently detected in the Reed- Sternberg (RS) cells of mixed cellularity Hodgkin's disease (37 of 48 [77%]) compared with the nodular sclerosis subtype (19 of 71 [27%], P < .001). The presence of EBV was also associated with Hispanic ethnicity (P < .001). In a multivariate analysis, patient age, gender, and geographic location were less predictive of EBV positivity than were mixed cellularity histology (odds ratio = 8.3) and Hispanic ethnicity (odds ratio = 4.3). Southern blot analysis of EBV terminal repeat fragments using the Xho1a probe showed that the viral DNA was monoclonal in 17 of 17 cases having EBER1-positive RS cells. By comparison, EBV DNA was not detected by Southern analysis in 20 cases lacking EBER1 in RS cells, even when occasional background lymphocytes expressed EBER1. Because clonal viral DNA was so readily detected in EBER1-positive cases, the EBV genome is probably amplified at least 50- fold in the infected RS cells. Monoclonality of EBV DNA implies that the RS cells were infected before malignant transformation.

scholarly journals Ekspresi Relatif mRNA LMP1 Epstein-Barr Virus dari Jaringan Tumor Terfiksasi Formalin dalam Blok Parafin sebagai Biomarker Progresivitas Tumor pada Karsinoma Nasofaring Tidak Berdiferensiasi

2019 ◽  
Vol 8 (1) ◽  
pp. 43-50
Author(s):  
Daniel Joko Wahyono ◽  
Arundito Widikusumo ◽  
Hidayat Sulistyo
Keyword(s):  
Cross Sectional ◽  
Relative Expression ◽  
Barr Virus ◽  
Ebv Infection ◽  
Stadium Iv ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
High Incidence Rate ◽  
Epstein Barr ◽  
Formalin Fixed

AbstractNasopharyngeal carcinoma (NPC) is a multifactorial disease that is geographically endemic in the world. Indonesia population is about 225 million people having varied diversity ethnic and has the high incidence rate of NPC approximately 6.2 per 100,000 people per year. EBV infection has been shown to be consistent with the onset of NPC. The pathogenesis of NPC is more directly reflected by carcinoma-specific viral transcriptional activity at the site of primary tumor. Therefore, EBV infection in NPC is also reflected by the expression of EBV latent and lytic gene, particularly in the expression of mRNA LMP1 EBV as a biomarker for EBV latent infection. The aims of this study were to determine the relative expression of mRNA LMP1 EBV in undifferentiated NPC (NPC WHO-III) patient as a biomarker of tumour progressivity in NPC. The samples were the tissue biopsies formalin-fixed embedded paraffin (FFPE) of 28 undifferentiated NPC patients at Pathology Anatomy Departement, R.S.U.D. Prof. dr. Margono Soekarjo, Purwokerto. The relative expression of mRNA LMP1 EBV was determined by RT-qPCR technique and calculated by formula 2-ΔCt. The relative expression of mRNA LMP1 EBV in undifferentiated NPCpatients on advanced staging was approximately start from 5,832.90 to 39,786.70. In undifferentiated NPC patients, the relative expression of mRNA LMP1 EBV in stadium IV (13.619,33 + 4902,3) increased of 1.5 times compared to stadium III (n = 2; 9.299,35 + 6178,9), however it was no significance differences statistically. Therefore, the relative expression of mRNA LMP1 EBV has a potensial biomarker of tumour progressivity in advanced staging undifferentiated NPC. AbstrakKarsinoma Nasofaring (KNF) merupakan penyakit yang bersifat multifaktor dan endemik di dunia. Populasi penduduk Indonesia yang berjumlah 225 juta yang terdiri atas beragam etnis populasi memiliki laju insidensi kasus KNF yang tinggi berkisar 6,2 per 100.000 penduduk pertahun. Infeksi EBV terbukti konsisten dengan perkembangan KNF. Patogenesis KNF lebih nyata terlihat dari aktivitas spesifik transkripsi virus pada tumor primernya. Infeksi EBV pada KNF dapat diamati dari ekspresi gen laten dan litiknya.. LMP1 merupakan onkogen utama dalam tumorigenesis KNF, sehingga ekspresi mRNA LMP1 EBV berperan sebagai petanda biologi infeksi laten EBV. Penelitian ini adalah untuk mengetahui ekspresi relatif mRNA LMP1 EBV berpotensi sebagai biomarka progresivitas tumor pada KNF tidak berdiferensiasi (KNF WHO III) Desain penelitian ini adalah studi cross sectional (seran lintang). Subyek penelitian adalah 28 biopsi jaringan tumor nasofaring yang terfiksasi dalam blok paraffin (formalin-fixed paraffin embedded/FFPE) populasi pasien KNF tidak berdiferensiasi pada Poli Patologi Anatomi R.S.U.D. Prof dr. Margono Soekarjo – Fakultas Kedokteran Unsoed, Purwokerto. Ekspresi relatif mRNA LMP1 EBV ditentukan dengan teknik RT-qPCR dan dihitung dengan rumus 2-ΔCt. Nilai ekspresi relatif mRNA LMP1 EBV pada penderita KNF tidak berdiferensiasi stadium lanjut berkisar 5,832.90 - 39,786.70. Nilai ekspresi relatif mRNA LMP1 EBV pada stadium IV (13.619,33 + 4902,3) meningkat 1,5 kali dibandingkan dengan stadium III (9.299,35 + 6178,9), namun peningkatan ekspresi relatif mRNA LMP1 EBV tidak bermakna secara statistik Ekspresi relatif mRNA LMP1 EBV dari biopsi jaringan tumor FFPE berpotensi sebagai biomarka progresivitas tumor pada stadium lanjut KNF tidak berdiferensiasi.

scholarly journals Nested Polymerase Chain Reaction and In Situ Hybridization for Diagnosis of Canine Herpesvirus Infection in Puppies

1998 ◽  
Vol 35 (3) ◽  
pp. 209-217 ◽  
Author(s):  
C. Schulze ◽  
W. Baumgärtner
Keyword(s):  
Epithelial Cells ◽  
Chain Reaction ◽  
Viral Dna ◽  
Nested Polymerase Chain Reaction ◽  
Canine Herpesvirus ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Polymerase Chain ◽  
Formalin Fixed

The usefulness of two nucleic acid detection systems in suspected cases of spontaneous canine herpesvirus (CHV) infection in puppies was evaluated. Formalin-fixed, paraffin-embedded tissues from seven 1–3-week-old naturally infected puppies with lesions characteristic of CHV infection were investigated in a retrospective study. Nested polymerase chain reaction (PCR) and nonradioactive in situ hybridization (ISH) were used to detect nucleotide sequences of the CHV thymidine kinase (TK) gene. According to the original necropsy reports, CHV was isolated in four of the seven puppies using primary canine lung and/or kidney cells. In all seven puppies, gross and histologic lesions consisted of disseminated focal necroses and hemorrhages predominantly in kidneys, lung, liver, and spleen. In addition, few small amphophilic intranuclear inclusion bodies were detected by light microscopy mainly in epithelial cells of kidney, lung, and liver. ISH was performed with a 111-base-pair (bp) digoxigenin-labeled double-stranded DNA probe. Viral DNA was detected in the nuclei of cells near and within lesions. Various cell types, including bronchiolar and alveolar epithelial cells, hepatocytes, renal tubular epithelial cells, neurons, fibrocytes, cardiac myocytes, and endothelial cells, were positive for viral DNA. PCR amplification products of the expected length of 168 bp containing the expected cleavage site for the restriction enzyme EcoRI, derived from paraffin blocks containing lung, kidney, and liver tissues, were detected in all seven puppies. The specificity of the obtained amplicon was further confirmed by Southern blot analysis. ISH and PCR are both useful methods for diagnosing CHV infection in formalin-fixed, paraffin-embedded tissues and are highly specific and sensitive methods for further investigations of the pathogenesis of CHV-induced lesions.

scholarly journals Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity

Blood ◽  
1994 ◽  
Vol 83 (6) ◽  
pp. 1595-1602 ◽  
Author(s):  
ML Gulley ◽  
PA Eagan ◽  
L Quintanilla-Martinez ◽  
AL Picado ◽  
BN Smir ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Odds Ratio ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Viral Dna ◽  
Mixed Cellularity ◽  
Hispanic Ethnicity ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

Abstract One hundred twenty-five cases of Hodgkin's disease from the United States (79), Mexico City (31), and Costa Rica (15) were analyzed for the presence of Epstein-Barr virus (EBV) by in situ hybridization to EBER1 transcripts. EBV was more frequently detected in the Reed- Sternberg (RS) cells of mixed cellularity Hodgkin's disease (37 of 48 [77%]) compared with the nodular sclerosis subtype (19 of 71 [27%], P < .001). The presence of EBV was also associated with Hispanic ethnicity (P < .001). In a multivariate analysis, patient age, gender, and geographic location were less predictive of EBV positivity than were mixed cellularity histology (odds ratio = 8.3) and Hispanic ethnicity (odds ratio = 4.3). Southern blot analysis of EBV terminal repeat fragments using the Xho1a probe showed that the viral DNA was monoclonal in 17 of 17 cases having EBER1-positive RS cells. By comparison, EBV DNA was not detected by Southern analysis in 20 cases lacking EBER1 in RS cells, even when occasional background lymphocytes expressed EBER1. Because clonal viral DNA was so readily detected in EBER1-positive cases, the EBV genome is probably amplified at least 50- fold in the infected RS cells. Monoclonality of EBV DNA implies that the RS cells were infected before malignant transformation.

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