Frequency of Epstein–Barr Virus DNA in Formalin-fixed Paraffin-embedded Tissue of Patients with Ductal Breast Carcinoma

Background: Nasopharyngeal carcinoma(NPC) is a rare malignant carcinoma that develops in the epithelial lining of the nasopharyngeal mucosa It is the most common neoplasm of the Nasopharynxand it is associated with many risk factors; one of them is Epstein-Barr virus infection. An Epstein-Barr virus is a tumorigenic herpes virus that infects and persists in B-lymphocytes without causing disease. This virus is associated with significant pathological conditions, such as benign and malignant lymphoproliferation. Objectives: To determine Epstein -Barr encoded RNA 1&2 (EBER1,2) and latent membrane protein (LMP) expressionin formalin-fixed paraffin-embedded tissue samples obtained from Omani patients diagnosed with nasopharyngeal carcinoma.Also, to identify the pattern and the type(s) of cells infected with EBV in nasopharyngeal carcinoma tissue samples obtained from Omani patients. Moreover, to compare the sensitivity of Immuno histochemistry and in-situ Hybridization for the detection of EBV in the nasopharyngeal carcinoma tissue sample. Materials and Method:Thirteen formalin-fixed paraffin-embedded nasopharyngeal carcinoma tissue samples archived from the period 2010 to 2017, were obtained from the Pathology Departments of Sultan Qaboos University Hospital and the Armed Force Hospital. These tissue samples were processed using two different methods Immunohistochemistry (IHC) and In situ hybridization (ISH). Results:Eleven out of thirteen NPC Omani patients were positive for EBV (84.61%) by either LMP-IHC or EBER-ISH. All cells stained positive for EBV in NPC tissue samples was of malignant type rather than normal cell type. EBV is mostly detected in patients in the age group of less than 50 years old. Also out of the 13 NPC patients, seven females (58.34%), and six males (46.15%) were positive for EBV. Conclusion:This study may provide evidence indicating an association between EBV and nasopharyngeal carcinoma. In addition, the detection of EBV in NPC obtained from Omani patients may encourage the physician to consider using anti-herpes virus drugs in the treatment of EBV positive NPC patients as an additional tool for the treatment of this kind of malignancy.

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High prevalence of Epstein-Barr virus in the Reed-Sternberg cells of Hodgkin's disease occurring in Peru

Blood ◽

10.1182/blood.v81.2.496.496 ◽

1993 ◽

Vol 81 (2) ◽

pp. 496-501 ◽

Cited By ~ 123

Author(s):

KL Chang ◽

PF Albujar ◽

YY Chen ◽

RM Johnson ◽

LM Weiss

Keyword(s):

In Situ Hybridization ◽

Epstein Barr Virus ◽

Lower Percentage ◽

Double Labeling ◽

Barr Virus ◽

Formalin Fixed Paraffin ◽

High Prevalence ◽

Formalin Fixed Paraffin Embedded ◽

Epstein Barr

Abstract The Epstein-Barr virus (EBV) has been implicated in the pathogenesis of Hodgkin‧s disease (HD). This study was undertaken to determine whether the association of EBV with HD showed geographical variation, as in Burkitt‧s lymphoma. We studied 32 formalin-fixed, paraffin-embedded cases of HD occurring in Peru. EBV DNA-RNA in situ hybridization was performed using a 30-base biotinylated antisense oligonucleotide complementary to the EBER1 gene of EBV. EBV immunohistochemistry was also performed, using a monoclonal antibody (MoAb) to the latent membrane protein (LMP1) of EBV. Identification of the precise cellular subset staining with EBV was accomplished via double-labeling with MoAbs directed against Reed-Sternberg cells (LeuM1/CD15) and B cells (L26/CD20). EBV RNA was identified in all or virtually all of the Reed- Sternberg cells and variants in 30 of the 32 (94%) cases of HD by in situ hybridization. LMP1 expression was identified in 83% of the EBER1- positive cases. Double-labeling studies confirmed the localization of EBV RNA to CD15-expressing Hodgkin‧s cells. This study found an extremely high prevalence of EBV in Peruvian HD, in contrast to the much lower percentage of EBV-associated cases of HD occurring in “Western” patients.

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Ekspresi Relatif mRNA LMP1 Epstein-Barr Virus dari Jaringan Tumor Terfiksasi Formalin dalam Blok Parafin sebagai Biomarker Progresivitas Tumor pada Karsinoma Nasofaring Tidak Berdiferensiasi

Jurnal Biotek Medisiana Indonesia ◽

10.22435/jbmi.v8i1.2582 ◽

2019 ◽

Vol 8 (1) ◽

pp. 43-50

Author(s):

Daniel Joko Wahyono ◽

Arundito Widikusumo ◽

Hidayat Sulistyo

Keyword(s):

Cross Sectional ◽

Relative Expression ◽

Barr Virus ◽

Ebv Infection ◽

Stadium Iv ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

High Incidence Rate ◽

Epstein Barr ◽

Formalin Fixed

AbstractNasopharyngeal carcinoma (NPC) is a multifactorial disease that is geographically endemic in the world. Indonesia population is about 225 million people having varied diversity ethnic and has the high incidence rate of NPC approximately 6.2 per 100,000 people per year. EBV infection has been shown to be consistent with the onset of NPC. The pathogenesis of NPC is more directly reflected by carcinoma-specific viral transcriptional activity at the site of primary tumor. Therefore, EBV infection in NPC is also reflected by the expression of EBV latent and lytic gene, particularly in the expression of mRNA LMP1 EBV as a biomarker for EBV latent infection. The aims of this study were to determine the relative expression of mRNA LMP1 EBV in undifferentiated NPC (NPC WHO-III) patient as a biomarker of tumour progressivity in NPC. The samples were the tissue biopsies formalin-fixed embedded paraffin (FFPE) of 28 undifferentiated NPC patients at Pathology Anatomy Departement, R.S.U.D. Prof. dr. Margono Soekarjo, Purwokerto. The relative expression of mRNA LMP1 EBV was determined by RT-qPCR technique and calculated by formula 2-ΔCt. The relative expression of mRNA LMP1 EBV in undifferentiated NPCpatients on advanced staging was approximately start from 5,832.90 to 39,786.70. In undifferentiated NPC patients, the relative expression of mRNA LMP1 EBV in stadium IV (13.619,33 + 4902,3) increased of 1.5 times compared to stadium III (n = 2; 9.299,35 + 6178,9), however it was no significance differences statistically. Therefore, the relative expression of mRNA LMP1 EBV has a potensial biomarker of tumour progressivity in advanced staging undifferentiated NPC. AbstrakKarsinoma Nasofaring (KNF) merupakan penyakit yang bersifat multifaktor dan endemik di dunia. Populasi penduduk Indonesia yang berjumlah 225 juta yang terdiri atas beragam etnis populasi memiliki laju insidensi kasus KNF yang tinggi berkisar 6,2 per 100.000 penduduk pertahun. Infeksi EBV terbukti konsisten dengan perkembangan KNF. Patogenesis KNF lebih nyata terlihat dari aktivitas spesifik transkripsi virus pada tumor primernya. Infeksi EBV pada KNF dapat diamati dari ekspresi gen laten dan litiknya.. LMP1 merupakan onkogen utama dalam tumorigenesis KNF, sehingga ekspresi mRNA LMP1 EBV berperan sebagai petanda biologi infeksi laten EBV. Penelitian ini adalah untuk mengetahui ekspresi relatif mRNA LMP1 EBV berpotensi sebagai biomarka progresivitas tumor pada KNF tidak berdiferensiasi (KNF WHO III) Desain penelitian ini adalah studi cross sectional (seran lintang). Subyek penelitian adalah 28 biopsi jaringan tumor nasofaring yang terfiksasi dalam blok paraffin (formalin-fixed paraffin embedded/FFPE) populasi pasien KNF tidak berdiferensiasi pada Poli Patologi Anatomi R.S.U.D. Prof dr. Margono Soekarjo – Fakultas Kedokteran Unsoed, Purwokerto. Ekspresi relatif mRNA LMP1 EBV ditentukan dengan teknik RT-qPCR dan dihitung dengan rumus 2-ΔCt. Nilai ekspresi relatif mRNA LMP1 EBV pada penderita KNF tidak berdiferensiasi stadium lanjut berkisar 5,832.90 - 39,786.70. Nilai ekspresi relatif mRNA LMP1 EBV pada stadium IV (13.619,33 + 4902,3) meningkat 1,5 kali dibandingkan dengan stadium III (9.299,35 + 6178,9), namun peningkatan ekspresi relatif mRNA LMP1 EBV tidak bermakna secara statistik Ekspresi relatif mRNA LMP1 EBV dari biopsi jaringan tumor FFPE berpotensi sebagai biomarka progresivitas tumor pada stadium lanjut KNF tidak berdiferensiasi.

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Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR

European Journal of Histochemistry ◽

10.4081/958 ◽

2009 ◽

Vol 49 (3) ◽

pp. 309

Author(s):

N Marziliano ◽

E Arbustini ◽

M Rossi de Gasperis ◽

S Crovella

Keyword(s):

Epstein Barr Virus ◽

In Situ Pcr ◽

Barr Virus ◽

Formalin Fixed Paraffin ◽

Epstein Barr ◽

Formalin Fixed

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High prevalence of Epstein-Barr virus in the Reed-Sternberg cells of Hodgkin's disease occurring in Peru

Blood ◽

10.1182/blood.v81.2.496.bloodjournal812496 ◽

1993 ◽

Vol 81 (2) ◽

pp. 496-501 ◽

Cited By ~ 1

Author(s):

KL Chang ◽

PF Albujar ◽

YY Chen ◽

RM Johnson ◽

LM Weiss

Keyword(s):

In Situ Hybridization ◽

Epstein Barr Virus ◽

Lower Percentage ◽

Double Labeling ◽

Barr Virus ◽

Formalin Fixed Paraffin ◽

High Prevalence ◽

Formalin Fixed Paraffin Embedded ◽

Epstein Barr

The Epstein-Barr virus (EBV) has been implicated in the pathogenesis of Hodgkin‧s disease (HD). This study was undertaken to determine whether the association of EBV with HD showed geographical variation, as in Burkitt‧s lymphoma. We studied 32 formalin-fixed, paraffin-embedded cases of HD occurring in Peru. EBV DNA-RNA in situ hybridization was performed using a 30-base biotinylated antisense oligonucleotide complementary to the EBER1 gene of EBV. EBV immunohistochemistry was also performed, using a monoclonal antibody (MoAb) to the latent membrane protein (LMP1) of EBV. Identification of the precise cellular subset staining with EBV was accomplished via double-labeling with MoAbs directed against Reed-Sternberg cells (LeuM1/CD15) and B cells (L26/CD20). EBV RNA was identified in all or virtually all of the Reed- Sternberg cells and variants in 30 of the 32 (94%) cases of HD by in situ hybridization. LMP1 expression was identified in 83% of the EBER1- positive cases. Double-labeling studies confirmed the localization of EBV RNA to CD15-expressing Hodgkin‧s cells. This study found an extremely high prevalence of EBV in Peruvian HD, in contrast to the much lower percentage of EBV-associated cases of HD occurring in “Western” patients.

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LMP-1 IMMUNOHISTOCHEMICAL DETECTION OF EPSTEIN - BARR VIRUS IN LYMPHOMA AMONG SUDANESE PATIENTS

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v3.i9.2015.2946 ◽

2015 ◽

Vol 3 (9) ◽

pp. 63-69

Author(s):

Ibrahim BakhitYousif ◽

Yousif Mohammed Yousif ◽

SideegAbd Elaziz Mohammed

Keyword(s):

Epstein Barr Virus ◽

Sufficient Evidence ◽

Control Group ◽

Barr Virus ◽

Formalin Fixed Paraffin ◽

Significant Difference ◽

Formalin Fixed Paraffin Embedded ◽

Epstein Barr ◽

Lymphoma Study Group ◽

And Control

Several studies have suggested a probable etiologic association between Epstein-Barr virus (EBV) and lymphoma; multiple EBV proteins can be expressed in infected lymphocytes, among which Latent membrane protein-1(LMP-1) is thought to be most important for malignant transformation, the aim of this study was to investigate the association of LMP-1 EBV in this tumors. This study was carried at the Radio Isotope Center Khartoum (RICK)- Khartoum state –Sudan from January 2015 to July 2015, it was retrospective and prospective, the study include ninety (90) Formalin –fixed paraffin embedded biopsies from which seventy (70) of malignant lymphoma (study group) and twenty (20) lymph node hyperplasia (control group ). EBV was detected in (32%) (23/70), and (10%) (2/20) respectively, the (P.value =0.04), this result showed a significant difference between case and control groups, the, there is no statistically significant difference between the two lymphoma subtypes Hodgkin's and Non-Hodgkin`s the (P.value= 0.333). There is sufficient evidence for the carcinogenicity of EBV in the causation of lymphoma in Sudan. This requires further evaluation to find out whether this positivity is due to co-infection or has a role in pathogenesis.

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