High-level expression of fatty acid synthase in human prostate cancer tissues is linked to activation and nuclear localization of Akt/PKB

2005 ◽  
Vol 206 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Tine Van de Sande ◽  
Tania Roskams ◽  
Evelyne Lerut ◽  
Steven Joniau ◽  
Hein Van Poppel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Fatty Acid ◽  
Nuclear Localization ◽  
Fatty Acid Synthase ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
High Level Expression ◽  
High Level ◽  
Cancer Tissues

scholarly journals Citral Induced Apoptosis through Modulation of Key Genes Involved in Fatty Acid Biosynthesis in Human Prostate Cancer Cells: In Silico and In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Sri Renukadevi Balusamy ◽  
Haribalan Perumalsamy ◽  
Karpagam Veerappan ◽  
Md. Amdadul Huq ◽  
S. Rajeshkumar ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Fatty Acid Synthase ◽  
Human Prostate ◽  
Detection Methods ◽  
Potential Candidate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Apoptosis Pathway

The isomers of citral (cis-citral and trans-citral) were isolated from the Cymbopogon citratus (DC.) Stapf oil demonstrates many therapeutic properties including anticancer properties. However, the effects of citral on suppressing human prostate cancer and its underlying molecular mechanism have yet to be elucidated. The citral was isolated from lemongrass oil using various spectroscopic analyses, such as electron ionized mass spectrometry (EI-MS) and nuclear magnetic resonance (NMR) spectroscopy respectively. We carried out 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to evaluate the cell viability of citral in prostate cancer cells (PC-3 and PC3M). Furthermore, to confirm that PC3 undergoes apoptosis by inhibiting lipogenesis, we used several detection methods including flow cytometry, DNA fragmentation, Hoechst staining, PI staining, oil staining, qPCR, and Western blotting. Citral impaired the clonogenic property of the cancer cells and altered the morphology of cancer cells. Molecular interaction studies and the PASS biological program predicted that citral isomers tend to interact with proteins involved in lipogenesis and the apoptosis pathway. Furthermore, citral suppressed lipogenesis of prostate cancer cells through the activation of AMPK phosphorylation and downregulation of fatty acid synthase (FASN), acetyl coA carboxylase (ACC), 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), and sterol regulatory element-binding protein (SREBP1) and apoptosis of PC3 cells by upregulating BAX and downregulating Bcl-2 expression. In addition, in silico studies such as ADMET predicted that citral can be used as a safe potent drug for the treatment of prostate cancer. Our results indicate that citral may serve as a potential candidate against human prostate cancer and warrants in vivo studies.

Citrate enhances in vitro metastatic behaviours of PC-3M human prostate cancer cells: Status of endogenous citrate and dependence on aconitase and fatty acid synthase

2006 ◽  
Vol 38 (10) ◽  
pp. 1766-1777 ◽  
Author(s):  
Maria E. Mycielska ◽  
Timothy P. Broke-Smith ◽  
Christopher P. Palmer ◽  
Rachel Beckerman ◽  
Theodoros Nastos ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Fatty Acid Synthase ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells

Nuclear localization of protein kinase CK2 catalytic subunit (CK2α) is associated with poor prognostic factors in human prostate cancer

2007 ◽  
Vol 43 (5) ◽  
pp. 928-934 ◽  
Author(s):  
Mathieu Laramas ◽  
Dominique Pasquier ◽  
Odile Filhol ◽  
François Ringeisen ◽  
Jean-Luc Descotes ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prognostic Factors ◽  
Protein Kinase ◽  
Nuclear Localization ◽  
Protein Kinase Ck2 ◽  
Catalytic Subunit ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Kinase Ck2

scholarly journals Differential expression of proteins in human prostate cancer tissues probed by MALDI imaging mass spectrometry

2020 ◽  
Author(s):  
Amrita Mitra ◽  
Surya Kant Choubey ◽  
Rajdeep Das ◽  
Pritilata Rout ◽  
T. S Sridhar ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Expression Profiles ◽  
Imaging Mass Spectrometry ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Tissue Sections ◽  
Cancer Tissues

AbstractHistopathology, the gold-standard method for diagnosis of human prostate cancer, is based on the analysis of changes in cellular morphology and tissue architecture in chemically stained tissue sections. Even at the very early stages of cancer with minimal phenotypic changes in cellular morphologies, there might be detectable changes in the expression profiles of proteins. Over the last decade, imaging mass spectrometry has been used to explore the spatial distribution and expression profiles of several molecules with their twodimensional heterogeneity retained across the tissue section. In the present study, using MALDI mass spectrometry based tissue imaging, we report the differential expression of three proteins, vinculin, ribonuclease T2 and 60 kDa heat shock protein across human prostate tissue sections in regions pathologically demarcated as frankly malignant. In an independent analysis, quantitative proteomics revealed that in cancerous prostate tissues, ribonuclease T2 and 60 kDa heat shock protein were significantly overexpressed by 22.26- and 6 folds respectively compared to benign condition. Our results show the utility of this approach to probe differential protein expression in architecturally intact cancer tissues. In addition, we propose that ribonuclease T2 and 60 kDa heat shock protein might be developed as diagnostic biomarkers for prostate cancer in future.

scholarly journals Discrepancies between detection of bcl-2 byin situ hybridization and immunocytochemistry in human prostate cancer tissues

1998 ◽  
Vol 79 (6) ◽  
pp. 614-618 ◽  
Author(s):  
Michelangelo Fiorentino ◽  
Antonia D'Errico ◽  
Chiara Barozzi ◽  
Walter F. Grigioni
Keyword(s):  
Prostate Cancer ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Cancer Tissues

scholarly journals Large Oncosomes in Human Prostate Cancer Tissues and in the Circulation of Mice with Metastatic Disease

2012 ◽  
Vol 181 (5) ◽  
pp. 1573-1584 ◽  
Author(s):  
Dolores Di Vizio ◽  
Matteo Morello ◽  
Andrew C. Dudley ◽  
Peter W. Schow ◽  
Rosalyn M. Adam ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Metastatic Disease ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Cancer Tissues

scholarly journals Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection

Retrovirology ◽  
2011 ◽  
Vol 8 (1) ◽  
pp. 13 ◽  
Author(s):  
Jeremy A Garson ◽  
Paul Kellam ◽  
Greg J Towers
Keyword(s):  
Prostate Cancer ◽  
Human Infection ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Integration Sites ◽  
Cancer Tissues
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