Mutation‐ and methylation‐profiles of ectopic and eutopic endometrial epithelium

2021 ◽  
Author(s):  
Lihong Li ◽  
Maria Facadio Antero ◽  
Ming Zhang ◽  
Tiffany Chu ◽  
Tamer Seckin ◽  
...  
Keyword(s):  
Endometrial Epithelium

Receptor Binding Inhibitor Suppresses Carcinogenesis of Cervical Cancer by Depressing Levels of FSHR and ERβ in Mice

2019 ◽  
Vol 19 (14) ◽  
pp. 1719-1727
Author(s):  
Zhuandi Gong ◽  
Xiaoyun Shen ◽  
Juan Yang ◽  
Luju Lai ◽  
Suocheng Wei
Keyword(s):  
Cervical Cancer ◽  
Receptor Binding ◽  
Estrogen Receptor Beta ◽  
Initial Study ◽  
Uterine Wall ◽  
Fsh Receptor ◽  
Uterine Lumen ◽  
Maturation Rate ◽  
Endometrial Epithelium ◽  
And Control

Background: FSH Receptor Binding Inhibitor (FRBI) blocked the binding of FSH to FSHR. Our initial study revealed FRBI reduced the maturation rate, enhanced the apoptosis of sheep Cumulus-Oocyte Complex (COCs). Little is known about whether FRBI modulates ERβ and FSHR levels in the normal uterine and cancerous tissues. The present study aimed to evaluate the FRBI effects on the expressions of Estrogen Receptor-beta (ERβ) and FSH receptor (FSHR) in the uteri. Methods: Methods: 150 mice were assigned to FRBI+FSH (COM), FSH and control groups (CG). Mice of COM-1, COM-2 and COM-3 groups were simultaneously intramuscularly injected with 500, 750 and 1000 µg FRBI with 10 IU FSH, respectively for five days. Western blotting and qPCR were utilized to determine the expression of ERβ and FSHR. Results: In comparison with FSH group, uterine lumen and glands of COM groups became narrow. The uterine wall and endometrial epithelium were thinned, and uterine lumen became narrow. Epithelial cells were decreased. Uterine wall thicknesses of COM-1, COM-2 and COM-3 groups were reduced by 6.49%, 14.89% and 15.69% on day 30 as compared with FSH group. Uterine perimetrium thicknesses of COM-1, COM-2 and COM-3 groups were reduced by 16.17%, 17.93% and 19.92% on day 20 in comparison with FSH group. Levels of FSHR mRNAs and proteins of COM-1, COM-2 and COM-3 groups were less than FSH group on days 20 and 30 (P<0.05). ERβ protein of COM-3 group was less than FSH group. Serum estradiol (E2) and FSH concentrations of COM-2 and COM-3 were lower than that of FSH group on day 30. Conclusion: FRBI could decrease UWT and UPT, also block the uterine development, decline expression levels of ERβ and FSHR protein. Additionally, FRBI reduced the secretion of secretion of FSH and E2. Downregulating expression of FSHR and ERβ may be a potential treatment regimen for cervical cancer patients.

scholarly journals Disappearance of Anionic Sites from the Surface of the Rat Endometrial Epithelium at the Time of Blastocyst Implantation

1979 ◽  
Vol 21 (3) ◽  
pp. 691-707 ◽  
Author(s):  
Kathryn Hewitt ◽  
Alan E. Beer ◽  
Frederick Grinnell
Keyword(s):  
Anionic Sites ◽  
Blastocyst Implantation ◽  
Endometrial Epithelium

Morphologic Patterns of Human Endometrial Epithelium in Women with Uterine Myomata Treated with Leuprorelin Acetate

2010 ◽  
Vol 69 (2) ◽  
pp. 131-135
Author(s):  
Vittorio Palmara ◽  
Onofrio Triolo ◽  
Vincenzo Benedetto ◽  
Carmela Lo Re ◽  
Emanuele Sturlese ◽  
...  
Keyword(s):  
Leuprorelin Acetate ◽  
Endometrial Epithelium

scholarly journals Differential Expression Pattern of Goat Uterine Fluids Extracellular Vesicles miRNAs during Peri-Implantation

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2308
Author(s):  
Yanshe Xie ◽  
Guangbin Liu ◽  
Xupeng Zang ◽  
Qun Hu ◽  
Chen Zhou ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Small Rnas ◽  
Target Genes ◽  
Embryo Implantation ◽  
Mature Embryo ◽  
Luciferase Reporter ◽  
Small Rna Sequencing ◽  
Transmission Electron ◽  
Differential Expression Pattern ◽  
Endometrial Epithelium

Early pregnancy failure occurs when a mature embryo attaches to an unreceptive endometrium. During the formation of a receptive endometrium, extracellular vesicles (EVs) of the uterine fluids (UFs) deliver regulatory molecules such as small RNAs to mediate intrauterine communication between the embryo and the endometrium. However, profiling of small RNAs in goat UFs’ EVs during pregnancy recognition (day 16) has not been carried out. In this study, EVs were isolated from UFs on day 16 of the estrous cycle or gestation. They were isolated by Optiprep™ Density G radient (ODG) and verified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. Immunostaining demonstrated that CD63 was present both in the endometrial epithelium and glandular epithelium, and stain intensity was greater in the pregnant endometrium compared to the non-pregnant endometrium. Small RNA sequencing revealed that UFs’ EVs contained numerous sRNA families and a total of 106 differentially expressed miRNAs (DEMs). Additionally, 1867 target genes of the DEMs were obtained, and miRNA–mRNA interaction networks were constructed. GO and KEGG analysis showed that miRNAs were significantly associated with the formation of a receptive endometrium and embryo implantation. In addition, the fluorescence in situ hybridization assay (FISH) showed that chi-miR-451-5p was mainly expressed in stromal cells of the endometrium and a higher level was detected in the endometrial luminal epithelium in pregnant states. Moreover, the dual-luciferase reporter assay showed that chi-miR-451-5p directly binds to PSMB8 and may play an important role in the formation of a receptive endometrium and embryo implantation. In conclusion, these results reveal that UFs’ EVs contain various small RNAs that may be vital in the formation of a receptive endometrium and embryo implantation.

scholarly journals The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows

2020 ◽  
Author(s):  
Hong An ◽  
Bofan Fu ◽  
Xinxin Yao ◽  
Hemin Ni ◽  
Yong Guo ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Dairy Cows ◽  
Endometrial Epithelium

Non-neoplastic nuclear atypia in endometrial epithelium in postmenopausal women on hormonal therapy

2007 ◽  
Vol 50 (7) ◽  
pp. 958-961 ◽  
Author(s):  
C Feeley ◽  
S Rasbridge
Keyword(s):  
Postmenopausal Women ◽  
Hormonal Therapy ◽  
Nuclear Atypia ◽  
Endometrial Epithelium

scholarly journals Cytokeratin 8, 18 and 19 in endometrial epithelium of Norplant(R) and norethisterone enanthate injectable progestogen contraceptive users

1996 ◽  
Vol 11 (suppl 2) ◽  
pp. 144-149 ◽  
Author(s):  
S. Wonodirekso ◽  
W. Hadisaputra ◽  
B. Affandi ◽  
B. Siregar ◽  
P. A. W. Rogers
Keyword(s):  
Endometrial Epithelium

scholarly journals Oviductal glycoprotein 1 (OVGP1) is expressed by endometrial epithelium that regulates receptivity and trophoblast adhesion

2018 ◽  
Vol 35 (8) ◽  
pp. 1419-1429 ◽  
Author(s):  
Saniya Laheri ◽  
Nancy Ashary ◽  
Purvi Bhatt ◽  
Deepak Modi
Keyword(s):  
Endometrial Epithelium

scholarly journals Cells expressing PAX8 are the main source of homeostatic regeneration of adult mouse endometrial epithelium and give rise to serous endometrial carcinoma

2020 ◽  
Vol 13 (10) ◽  
pp. dmm047035
Author(s):  
Dah-Jiun Fu ◽  
Andrea J. De Micheli ◽  
Mallikarjun Bidarimath ◽  
Lora H. Ellenson ◽  
Benjamin D. Cosgrove ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Endometrial Carcinoma ◽  
Marrow Cell ◽  
Cell Types ◽  
Lineage Tracing ◽  
Cell Of Origin ◽  
Mouse Uterus ◽  
Endometrial Epithelium

ABSTRACTHumans and mice have cyclical regeneration of the endometrial epithelium. It is expected that such regeneration is ensured by tissue stem cells, but their location and hierarchy remain debatable. A number of recent studies have suggested the presence of stem cells in the mouse endometrial epithelium. At the same time, it has been reported that this tissue can be regenerated by stem cells of stromal/mesenchymal or bone marrow cell origin. Here, we describe a single-cell transcriptomic atlas of the main cell types of the mouse uterus and epithelial subset transcriptome and evaluate the contribution of epithelial cells expressing the transcription factor PAX8 to the homeostatic regeneration and malignant transformation of adult endometrial epithelium. According to lineage tracing, PAX8+ epithelial cells are responsible for long-term maintenance of both luminal and glandular epithelium. Furthermore, multicolor tracing shows that individual glands and contiguous areas of luminal epithelium are formed by clonal cell expansion. Inactivation of the tumor suppressor genes Trp53 and Rb1 in PAX8+ cells, but not in FOXJ1+ cells, leads to the formation of neoplasms with features of serous endometrial carcinoma, one of the most aggressive types of human endometrial malignancies. Taken together, our results show that the progeny of single PAX8+ cells represents the main source of regeneration of the adult endometrial epithelium. They also provide direct experimental genetic evidence for the key roles of the P53 and RB pathways in the pathogenesis of serous endometrial carcinoma and suggest that PAX8+ cells represent the cell of origin of this neoplasm.

scholarly journals Trophoblast attachment to the endometrial epithelium elicits compartment-specific transcriptional waves in an in-vitro model

2020 ◽  
Author(s):  
Paula Vergaro ◽  
Gustavo Tiscornia ◽  
Filippo Zambelli ◽  
Amelia Rodríguez ◽  
Josep Santaló ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Endometrial Epithelium ◽  
Vitro Model
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