Yellow starthistle (YST), Centaurea solstitialis L., is a weedy plant that is widely distributed in the Krasnodar Region of Russia. It is also an aggressive invasive weed in the western United States and a target of biological control efforts. In the summer of 2006, several hundred diseased plants were found near Taman, Russia. Symptoms of the disease were yellow, water-soaked leaf spots. Diseased leaves were collected, air dried, and sent to the Russian State Collection of Phytopathogenic Organisms at the All Russia Institute of Phytopathology (ARIP). The fungus isolated from the diseased leaves conformed to Periconia igniaria E.W. Mason & M.B. Ellis (teleomorph Didymosphaeria igniaria C. Booth) (1). Colonies of the fungus grew rapidly on potato glucose nutrition medium with aerial mycelium from fluffy to pressed and colorless at the beginning and darkening to black with age. The medium side of the colonies gradually became violet purple to wine colored. Conidiophores had aerial mycelia as much as 550 μm long and 9 to 13 μm wide tapering to 6 to 10 μm. Conidiophores were dark with short, swollen branched stipes. Conidia, formed in short twisted chains, were spherical, dark brown, 7 to 9 μm in diameter, and covered by 1 μm long spines. Yellow starthistle plants were grown in growth chambers with day/night air temperatures of 26 to 28/20 to 22°C, 60 to 70% relative air humidity, and 10,000 lx light for 16 h. Fifteen plants in the rosette stage were spray inoculated with an aqueous suspension of P. igniaria conidia at 5 × 106 conidia/ml and 5 ml per plant. Disease on leaves was observed on all plants 3 to 4 weeks after inoculation when the plants started to bolt. When the plants reached flowering stage, diffused yellow spots were observed on stems and inflorescences and all flowers died. Diseased leaves were surface disinfested and put on potato saccharose nutrition medium. P. igniaria was reisolated from 3 to 5 leaves of each plant and from flowers and stems that developed from 10 inoculated rosettes. Flowers of 10 YST plants were also inoculated with P. igniaria isolated from the previously inoculated plants. Disease developed in the flowers of all inoculated plants, and the symptoms were identical to those observed when rosettes were inoculated and disease followed bolting and flowering. No symptoms developed on four noninoculated plants included in each test. Internal transcribed spacer (ITS) sequences of the fungus were obtained and compared with sequences from GenBank. An uncultured soil fungus and three isolates of P. macrospinosa Lefebvre & Aar.G. Johnson produced the best homology (96%). No sequences for P. igniaria were available for comparison, but the description of P. macrospinosa (conidia 18 to 32 μm in diameter with 2.5 to 6 μm long spines) is clearly different than our isolate. ITS sequences for our isolate have been deposited in GenBank (Accession No. EU367468) and a voucher specimen has been deposited with the U.S. National Fungus Collection (BPI 878355). To our knowledge, this is the first report of P. igniaria causing disease on YST. Live cultures are being maintained at the Russian State Collection of Phytopathogenic Organisms in ARIP. Reference: (1) M. B. Ellis. Dematiaceous Hyphomycetes. CMI, Kew, UK, 1971.
Identifying volatile in vitro biomarkers for oral bacteria with proton-transfer-reaction mass spectrometry and gas chromatography–mass spectrometry
