Microfluidic assembly of uniform fluorescent microbeads from quantum-dot-loaded fluorine-containing microemulsion

Fluorescent quantum dots have been used in biological applications as desirable fluorescent labels instead of traditional fluorophores. Incorporation into microspheres enhances many features of quantum dots that make them ideal for biological detection, such as photostability, multi-target, and improved brightness. Quantum dot-tagged microbeads are emerging as a new class of fluorescent labels and are expected to open new opportunities in nanotechnology and biology. In this review, we describe different approaches for the synthesis of quantum dot-tagged microbeads, surface modification methods that make microbeads suitable for bioconjugation, and the biological applications of the quantum dot-tagged fluorescent microbeads with their desired features in recent research. We also discuss the limitations of some kinds of quantum dot-tagged microbeads and the developments that will enhance their abilities in biological applications.

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Synthesis and Application of Quantum Dot-Tagged Fluorescent Microbeads

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2008.308 ◽

2008 ◽

Vol 8 (3) ◽

pp. 1138-1149 ◽

Cited By ~ 8

Author(s):

Qiang Ma ◽

Chao Wang ◽

Xingguang Su

Keyword(s):

Quantum Dot ◽

Fluorescent Microbeads

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Microfluidic Assembly of Quantum Dot Micelles

Volume 11: Micro and Nano Systems, Parts A and B ◽

10.1115/imece2007-42300 ◽

2007 ◽

Author(s):

Greg Schabas ◽

Matthew G. Moffitt ◽

David Sinton

Keyword(s):

Particle Size ◽

Block Copolymer ◽

Quantum Dot ◽

Self Assembly ◽

Cds Quantum Dot ◽

Microfluidic Assembly ◽

On Chip ◽

Polymer Stabilized ◽

Step Control ◽

Chip Analysis

The controlled self-assembly of polymer-stabilized CdS quantum dot nanoparticles into quantum dot compound micelles (QDCMs) using microfluidics is demonstrated. In a flow-focusing configuration, water is introduced to a blend solution of block copolymer-stabilized quantum dots with amphiphilic block copolymer stabilizing chains. QDCM assembly via initial phase separation and subsequent agglomeration continue until a downstream quench step. Control over mean particle size and size distribution characteristics is demonstrated via both inlet concentrations and flow rate. The QDCMs assembled through this method are stable in aqueous solutions, and show internal and external structure in keeping with previous assembly methods. The on-chip evolution of QDCM formation and growth is resolved through fluorescence scattering. Particle size distributions and associated statistics are determined through off-chip analysis.

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Direct measurement of local field factors for second-harmonic generation from quantum dot Langmuir monolayers compressed through the metal-insulator transition

Philosophical Magazine B ◽

10.1080/014186399256637 ◽

1999 ◽

Vol 79 (9) ◽

pp. 1299-1305

Author(s):

C. P. Collier, S. Henrichs, J. R. Heat

Keyword(s):

Quantum Dot ◽

Second Harmonic Generation ◽

Direct Measurement ◽

Harmonic Generation ◽

Local Field ◽

Second Harmonic ◽

Langmuir Monolayers ◽

Insulator Transition ◽

Metal Insulator Transition ◽

Metal Insulator

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Biodistribution 18F-markierter Quantum Dot-Antikörper-Biokonjugate

10.1055/s-0039-1683631 ◽

2019 ◽

Author(s):

O Mishchenko ◽

A Schildan ◽

O Sabri ◽

M Patt

Keyword(s):

Quantum Dot

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Synchronization of Chaotic Quantum Dot Light Emitting Diodes under Optical Feedback Effect

10.32792/utq/utjsci/vol7/2/31 ◽

2020 ◽

pp. 144-148

Keyword(s):

Quantum Dot ◽

Delay Time ◽

Chaos Synchronization ◽

Optical Feedback ◽

Light Emitting Diode ◽

Feedback Effect ◽

Complete Synchronization ◽

Light Emitting ◽

Coupling Methods ◽

Coupling Parameters

Chaos synchronization of delayed quantum dot light emitting diode has been studied theortetically which are coupled via the unidirectional and bidirectional. at synchronization of chaotic, The dynamics is identical with delayed optical feedback for those coupling methods. Depending on the coupling parameters and delay time the system exhibits complete synchronization, . Under proper conditions, the receiver quantum dot light emitting diode can be satisfactorily synchronized with the transmitter quantum dot light emitting diode due to the optical feedback effect.

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Vertical-cavity emitting devices with quantum-dot structures

Uspekhi Fizicheskih Nauk ◽

10.3367/ufnr.0171.200108d.0855 ◽

2001 ◽

Vol 171 (8) ◽

pp. 855

Author(s):

Viktor M. Ustinov ◽

N.A. Maleev ◽

Aleksei E. Zhukov ◽

A.R. Kovsh ◽

A.V. Sakharov ◽

...

Keyword(s):

Quantum Dot ◽

Vertical Cavity

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Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

MRS Proceedings ◽

10.1557/proc-773-n7.9 ◽

2003 ◽

Vol 773 ◽

Author(s):

Aaron R. Clapp ◽

Igor L. Medintz ◽

J. Matthew Mauro ◽

Hedi Mattoussi

Keyword(s):

Energy Transfer ◽

Quantum Dot ◽

Organic Dyes ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Genetically Engineered ◽

Molar Ratio ◽

Binding Assays ◽

Specific Sequence ◽

Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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