Comparison of two-dimensional gel electrophoresis based and shotgun strategies in the study of plasma membrane proteome

2007 ◽  
Vol 1 (2) ◽  
pp. 231-241 ◽  
Author(s):  
Jintang He ◽  
Yashu Liu ◽  
Sizhi He ◽  
Qingsong Wang ◽  
Hai Pu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Membrane Proteome ◽  
Two Dimensional Gel Electrophoresis

Construction of a directory of tobacco plasma membrane proteins by combined two-dimensional gel electrophoresis and protein sequencing

1997 ◽  
Vol 18 (3-4) ◽  
pp. 654-660 ◽  
Author(s):  
David Rouquié ◽  
Jean-Beno??t Peltier ◽  
Monique Marquis-Mansion ◽  
Colette Tournaire ◽  
Patrick Doumas ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Gel Electrophoresis ◽  
Protein Sequencing ◽  
Two Dimensional ◽  
Plasma Membrane Proteins ◽  
Two Dimensional Gel Electrophoresis

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

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