scholarly journals Electron-capture dissociation and ion mobility mass spectrometry for characterization of the hemoglobin protein assembly

2015 ◽  
Vol 24 (8) ◽  
pp. 1325-1332 ◽  
Author(s):  
Weidong Cui ◽  
Hao Zhang ◽  
Robert E. Blankenship ◽  
Michael L. Gross
Keyword(s):  
Mass Spectrometry ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Protein Assembly ◽  
Ion Mobility Mass Spectrometry

scholarly journals Electron capture dissociation and drift tube ion mobility-mass spectrometry coupled with site directed mutations provide insights into the conformational diversity of a metamorphic protein

2015 ◽  
Vol 17 (16) ◽  
pp. 10538-10550 ◽  
Author(s):  
Sophie R. Harvey ◽  
Massimiliano Porrini ◽  
Robert C. Tyler ◽  
Cait E. MacPhee ◽  
Brian F. Volkman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Drift Tube ◽  
Ion Mobility Mass Spectrometry ◽  
Top Down ◽  
Conformational Diversity ◽  
Metamorphic Protein

Ion mobility mass spectrometry can be combined with data from top-down sequencing to discern adopted conformations of proteins in the absence of solvent.

scholarly journals Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry

2021 ◽  
Author(s):  
Kevin Jeanne Dit Fouque ◽  
Desmond Kaplan ◽  
Valery G. Voinov ◽  
Frederik H. V. Holck ◽  
Ole N. Jensen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Trapped Ion

scholarly journals Ion Mobility–Mass Spectrometry for Bioanalysis

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 33
Author(s):  
Xavier Garcia ◽  
Maria del Mar Sabaté ◽  
Jorge Aubets ◽  
Josep Maria Jansat ◽  
Sonia Sentellas
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Biological Samples ◽  
Ion Mobility Mass Spectrometry ◽  
Exogenous Compounds ◽  
Analytical Separation

This paper aims to cover the main strategies based on ion mobility spectrometry (IMS) for the analysis of biological samples. The determination of endogenous and exogenous compounds in such samples is important for the understanding of the health status of individuals. For this reason, the development of new approaches that can be complementary to the ones already established (mainly based on liquid chromatography coupled to mass spectrometry) is welcomed. In this regard, ion mobility spectrometry has appeared in the analytical scenario as a powerful technique for the separation and characterization of compounds based on their mobility. IMS has been used in several areas taking advantage of its orthogonality with other analytical separation techniques, such as liquid chromatography, gas chromatography, capillary electrophoresis, or supercritical fluid chromatography. Bioanalysis is not one of the areas where IMS has been more extensively applied. However, over the last years, the interest in using this approach for the analysis of biological samples has clearly increased. This paper introduces the reader to the principles controlling the separation in IMS and reviews recent applications using this technique in the field of bioanalysis.

scholarly journals Structural Characterization of Methylenedianiline Regioisomers by Ion Mobility-Mass Spectrometry and Tandem Mass Spectrometry. 4. 3-Ring and 4-Ring Isomers

2018 ◽  
Vol 90 (24) ◽  
pp. 14453-14461 ◽  
Author(s):  
Tiffany M. Crescentini ◽  
Sarah M. Stow ◽  
Jay G. Forsythe ◽  
Jody C. May ◽  
John A. McLean ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Structural Characterization ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Tandem Mass

scholarly journals A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry

2021 ◽  
Vol 12 ◽  
Author(s):  
Kai P. Law ◽  
Wei He ◽  
Jianchang Tao ◽  
Chuanlun Zhang
Keyword(s):  
Mass Spectrometry ◽  
Cross Sections ◽  
Ion Mobility ◽  
Resolving Power ◽  
Ion Mobility Mass Spectrometry ◽  
Ion Chemistry ◽  
Lipid Species ◽  
Mobility System ◽  
Archaeal Lipids

Archaea are differentiated from the other two domains of life by their biomolecular characteristics. One such characteristic is the unique structure and composition of their lipids. Characterization of the whole set of lipids in a biological system (the lipidome) remains technologically challenging. This is because the lipidome is innately complex, and not all lipid species are extractable, separable, or ionizable by a single analytical method. Furthermore, lipids are structurally and chemically diverse. Many lipids are isobaric or isomeric and often indistinguishable by the measurement of mass or even their fragmentation spectra. Here we developed a novel analytical protocol based on liquid chromatography ion mobility mass spectrometry to enhance the coverage of the lipidome and characterize the conformations of archaeal lipids by their collision cross-sections (CCSs). The measurements of ion mobility revealed the gas-phase ion chemistry of representative archaeal lipids and provided further insights into their attributions to the adaptability of archaea to environmental stresses. A comprehensive characterization of the lipidome of mesophilic marine thaumarchaeon, Nitrosopumilus maritimus (strain SCM1) revealed potentially an unreported phosphate- and sulfate-containing lipid candidate by negative ionization analysis. It was the first time that experimentally derived CCS values of archaeal lipids were reported. Discrimination of crenarchaeol and its proposed stereoisomer was, however, not achieved with the resolving power of the SYNAPT G2 ion mobility system, and a high-resolution ion mobility system may be required for future work. Structural and spectral libraries of archaeal lipids were constructed in non-vendor-specific formats and are being made available to the community to promote research of Archaea by lipidomics.

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