Applications of Ion Mobility Mass Spectrometry for Characterization of Protein Therapeutics

2013 ◽  
pp. 371-401
Author(s):  
Weibin Chen ◽  
Asish Chakraborty
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Protein Therapeutics ◽  
Ion Mobility Mass Spectrometry

scholarly journals Ion Mobility–Mass Spectrometry for Bioanalysis

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 33
Author(s):  
Xavier Garcia ◽  
Maria del Mar Sabaté ◽  
Jorge Aubets ◽  
Josep Maria Jansat ◽  
Sonia Sentellas
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Biological Samples ◽  
Ion Mobility Mass Spectrometry ◽  
Exogenous Compounds ◽  
Analytical Separation

This paper aims to cover the main strategies based on ion mobility spectrometry (IMS) for the analysis of biological samples. The determination of endogenous and exogenous compounds in such samples is important for the understanding of the health status of individuals. For this reason, the development of new approaches that can be complementary to the ones already established (mainly based on liquid chromatography coupled to mass spectrometry) is welcomed. In this regard, ion mobility spectrometry has appeared in the analytical scenario as a powerful technique for the separation and characterization of compounds based on their mobility. IMS has been used in several areas taking advantage of its orthogonality with other analytical separation techniques, such as liquid chromatography, gas chromatography, capillary electrophoresis, or supercritical fluid chromatography. Bioanalysis is not one of the areas where IMS has been more extensively applied. However, over the last years, the interest in using this approach for the analysis of biological samples has clearly increased. This paper introduces the reader to the principles controlling the separation in IMS and reviews recent applications using this technique in the field of bioanalysis.

scholarly journals Structural Characterization of Methylenedianiline Regioisomers by Ion Mobility-Mass Spectrometry and Tandem Mass Spectrometry. 4. 3-Ring and 4-Ring Isomers

2018 ◽  
Vol 90 (24) ◽  
pp. 14453-14461 ◽  
Author(s):  
Tiffany M. Crescentini ◽  
Sarah M. Stow ◽  
Jay G. Forsythe ◽  
Jody C. May ◽  
John A. McLean ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Structural Characterization ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Tandem Mass

scholarly journals A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry

2021 ◽  
Vol 12 ◽  
Author(s):  
Kai P. Law ◽  
Wei He ◽  
Jianchang Tao ◽  
Chuanlun Zhang
Keyword(s):  
Mass Spectrometry ◽  
Cross Sections ◽  
Ion Mobility ◽  
Resolving Power ◽  
Ion Mobility Mass Spectrometry ◽  
Ion Chemistry ◽  
Lipid Species ◽  
Mobility System ◽  
Archaeal Lipids

Archaea are differentiated from the other two domains of life by their biomolecular characteristics. One such characteristic is the unique structure and composition of their lipids. Characterization of the whole set of lipids in a biological system (the lipidome) remains technologically challenging. This is because the lipidome is innately complex, and not all lipid species are extractable, separable, or ionizable by a single analytical method. Furthermore, lipids are structurally and chemically diverse. Many lipids are isobaric or isomeric and often indistinguishable by the measurement of mass or even their fragmentation spectra. Here we developed a novel analytical protocol based on liquid chromatography ion mobility mass spectrometry to enhance the coverage of the lipidome and characterize the conformations of archaeal lipids by their collision cross-sections (CCSs). The measurements of ion mobility revealed the gas-phase ion chemistry of representative archaeal lipids and provided further insights into their attributions to the adaptability of archaea to environmental stresses. A comprehensive characterization of the lipidome of mesophilic marine thaumarchaeon, Nitrosopumilus maritimus (strain SCM1) revealed potentially an unreported phosphate- and sulfate-containing lipid candidate by negative ionization analysis. It was the first time that experimentally derived CCS values of archaeal lipids were reported. Discrimination of crenarchaeol and its proposed stereoisomer was, however, not achieved with the resolving power of the SYNAPT G2 ion mobility system, and a high-resolution ion mobility system may be required for future work. Structural and spectral libraries of archaeal lipids were constructed in non-vendor-specific formats and are being made available to the community to promote research of Archaea by lipidomics.

Design, Synthesis, and Traveling Wave Ion Mobility Mass Spectrometry Characterization of Iron(II)– and Ruthenium(II)–Terpyridine Metallomacrocycles

2011 ◽  
Vol 133 (31) ◽  
pp. 11967-11976 ◽  
Author(s):  
Yi-Tsu Chan ◽  
Xiaopeng Li ◽  
Jing Yu ◽  
Gustavo A. Carri ◽  
Charles N. Moorefield ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Traveling Wave ◽  
Ion Mobility Mass Spectrometry ◽  
Design Synthesis ◽  
Traveling Wave Ion Mobility

Petroleomics by ion mobility mass spectrometry: resolution and characterization of contaminants and additives in crude oils and petrofuels

2015 ◽  
Vol 7 (11) ◽  
pp. 4450-4463 ◽  
Author(s):  
Jandyson Machado Santos ◽  
Renan de S. Galaverna ◽  
Marcos A. Pudenzi ◽  
Eduardo M. Schmidt ◽  
Nathaniel L. Sanders ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Crude Oil ◽  
Ion Mobility ◽  
Drift Tube ◽  
Crude Oils ◽  
Ion Mobility Mass Spectrometry ◽  
Uniform Field ◽  
Field Drift

IM-MS, was performed in a new uniform-field drift tube, IM-QTOF instrument. It can resolve and characterize crude oil and its contaminants, as well as petrofuels and their additives.

Liquid chromatography–drift tube ion mobility–mass spectrometry as a new challenging tool for the separation and characterization of silymarin flavonolignans

2020 ◽  
Vol 412 (4) ◽  
pp. 819-832 ◽  
Author(s):  
Marie Fenclova ◽  
Milena Stranska-Zachariasova ◽  
Frantisek Benes ◽  
Alena Novakova ◽  
Petra Jonatova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility ◽  
Drift Tube ◽  
Ion Mobility Mass Spectrometry
Sign in / Sign up

Export Citation Format

Share Document