DNA‐PKc inhibition overcomes taxane resistance by promoting taxane‐induced DNA damage in prostate cancer cells

The Prostate ◽  
2021 ◽  
Author(s):  
Olivia S. Chao ◽  
Oscar B. Goodman
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Taxane Resistance

scholarly journals Piperlongumine inhibits migration and proliferation of castration-resistant prostate cancer cells via triggering persistent DNA damage

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ding-fang Zhang ◽  
Zhi-chun Yang ◽  
Jian-qiang Chen ◽  
Xiang-xiang Jin ◽  
Yin-da Qiu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Dna Damage ◽  
Cell Adhesion ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Castration Resistant Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Dna Damage And Repair ◽  
Castration Resistant

Abstract Background Metastatic castration-resistant prostate cancer (CRPC) is the leading cause of death among men diagnosed with prostate cancer. Piperlongumine (PL) is a novel potential anticancer agent that has been demonstrated to exhibit anticancer efficacy against prostate cancer cells. However, the effects of PL on DNA damage and repair against CRPC have remained unclear. The aim of this study was to further explore the anticancer activity and mechanisms of action of PL against CRPC in terms of DNA damage and repair processes. Methods The effect of PL on CRPC was evaluated by MTT assay, long-term cell proliferation, reactive oxygen species assay, western blot assay, flow cytometry assay (annexin V/PI staining), β-gal staining assay and DAPI staining assay. The capacity of PL to inhibit the invasion and migration of CRPC cells was assessed by scratch-wound assay, cell adhesion assay, transwell assay and immunofluorescence (IF) assay. The effect of PL on DNA damage and repair was determined via IF assay and comet assay. Results The results showed that PL exhibited stronger anticancer activity against CRPC compared to that of taxol, cisplatin (DDP), doxorubicin (Dox), or 5-Fluorouracil (5-FU), with fewer side effects in normal cells. Importantly, PL treatment significantly decreased cell adhesion to the extracellular matrix and inhibited the migration of CRPC cells through affecting the expression and distribution of focal adhesion kinase (FAK), leading to concentration-dependent inhibition of CRPC cell proliferation and concomitantly increased cell death. Moreover, PL treatment triggered persistent DNA damage and provoked strong DNA damage responses in CRPC cells. Conclusion Collectively, our findings demonstrate that PL potently inhibited proliferation, migration, and invasion of CRPC cells and that these potent anticancer effects were potentially achieved via triggering persistent DNA damage in CRPC cells.

scholarly journals RNA sequencing reveals upregulation of a transcriptomic program associated with stemness in metastatic prostate cancer cells selected for taxane resistance

Oncotarget ◽  
2018 ◽  
Vol 9 (54) ◽  
pp. 30363-30384 ◽  
Author(s):  
Christina K. Cajigas-Du Ross ◽  
Shannalee R. Martinez ◽  
Leanne Woods-Burnham ◽  
Alfonso M. Durán ◽  
Sourav Roy ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Rna Sequencing ◽  
Metastatic Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Taxane Resistance

scholarly journals Correction: Cyclin D1 silencing suppresses tumorigenicity, impairs DNA double strand break repair and thus radiosensitizes androgen-independent prostate cancer cells to DNA damage

Oncotarget ◽  
2016 ◽  
Vol 7 (39) ◽  
pp. 64526-64526 ◽  
Author(s):  
Francesco Marampon ◽  
Giovanni Luca Gravina ◽  
Xiaoming Ju ◽  
Antonella Vetuschi ◽  
Roberta Sferra ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cyclin D1 ◽  
Cancer Cells ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Prostate Cancer Cells ◽  
Dna Double Strand Break ◽  
Androgen Independent

Effects of titanocene dichloride derivatives on prostate cancer cells, specifically DNA damage-induced apoptosis

The Prostate ◽  
2010 ◽  
Vol 71 (2) ◽  
pp. 111-124 ◽  
Author(s):  
Sandra Cuffe ◽  
Catherine M. Dowling ◽  
James Claffey ◽  
Clara Pampillón ◽  
Megan Hogan ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Titanocene Dichloride ◽  
Prostate Cancer Cells ◽  
Induced Apoptosis

Abstract 334: Mcl-1 protects prostate cancer cells from chemotherapy-induced DNA damage

2014 ◽  
Author(s):  
Carlos Perez-Stable ◽  
Teresita Reiner ◽  
Alicia de las Pozas ◽  
Ricardo Parrondo
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Prostate Cancer Cells

scholarly journals Eupalinolide J induces apoptosis, cell cycle arrest, mitochondrial membrane potential disruption and DNA damage in human prostate cancer cells

2020 ◽  
Vol 45 (1) ◽  
pp. 15-23
Author(s):  
Zeqi Wu ◽  
Xintong Xu ◽  
Lingjie Dai ◽  
Yiqi Wang ◽  
Bo Yang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Cycle ◽  
Dna Damage ◽  
Membrane Potential ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Mitochondrial Membrane ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Cycle Arrest

Mitochondrial DNA damage is sensitive to exogenous H2O2 but independent of cellular ROS production in prostate cancer cells

2011 ◽  
Vol 716 (1-2) ◽  
pp. 40-50 ◽  
Author(s):  
Sam W. Chan ◽  
Phuong-Nam Nguyen ◽  
David Ayele ◽  
Simone Chevalier ◽  
Armen Aprikian ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Mitochondrial Dna ◽  
Cancer Cells ◽  
Ros Production ◽  
Prostate Cancer Cells ◽  
Mitochondrial Dna Damage ◽  
Exogenous H2o2

scholarly journals PARP inhibitor veliparib and HDAC inhibitor SAHA synergistically co-target the UHRF1/BRCA1 DNA damage repair complex in prostate cancer cells

2018 ◽  
Vol 37 (1) ◽  
Author(s):  
Linglong Yin ◽  
Youhong Liu ◽  
Yuchong Peng ◽  
Yongbo Peng ◽  
Xiaohui Yu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Hdac Inhibitor ◽  
Parp Inhibitor ◽  
Dna Damage Repair ◽  
Prostate Cancer Cells ◽  
Damage Repair
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