Amplified esterase genes and their relationship with other insecticide resistance mechanisms in English field populations of the aphid,Myzus persicae (Sulzer)

AbstractIntensive chemical treatments have led to the development of a number of insecticide resistance mechanisms in the peach–potato aphid Myzus persicae (Sulzer). Some of these mechanisms are known to be associated with negative pleiotropic effects (resistance costs). Molecular and biochemical methods were used to determine the genotypes or phenotypes associated with four insecticide resistance mechanisms in single aphids from sexually-reproducing populations in southern France. The mechanisms considered were E4 and FE4 carboxylesterase overproduction, modified acetycholinesterase, and kdr and rdl resistance-associated mutations. A new method for determining individual kdr genotypes is presented. Almost all resistant individuals overproduced FE4 carboxylesterase, whereas modified acetylcholinesterase was rare. Both the kdr and rdl resistance mutations were present at high frequencies in French sexually-reproducing populations. The frequencies of insecticide resistance genes were compared before and after sexual reproduction in one peach orchard at Avignon to evaluate the potential impact of selection on the persistence of resistance alleles in the over-wintering phase. The frequencies of the kdr and rdl mutations varied significantly between autumn and spring sampling periods. The frequency of the kdr mutation increased, probably due to pyrethroid treatments at the end of the winter. Conversely, the frequency of the rdl mutation decreased significantly during winter, probably because of a fitness cost associated with this mutation.

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Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms

Pest Management Science ◽

10.1002/ps.4036 ◽

2015 ◽

Vol 72 (4) ◽

pp. 671-683 ◽

Cited By ~ 21

Author(s):

Costas Ch Voudouris ◽

Amalia N Kati ◽

Eldem Sadikoglou ◽

Martin Williamson ◽

Panagiotis J Skouras ◽

...

Keyword(s):

Insecticide Resistance ◽

Myzus Persicae ◽

Resistance Mechanisms

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Insecticide Resistance Mechanisms in the Green Peach Aphid Myzus persicae (Hemiptera: Aphididae) I: A Transcriptomic Survey

PLoS ONE ◽

10.1371/journal.pone.0036366 ◽

2012 ◽

Vol 7 (6) ◽

pp. e36366 ◽

Cited By ~ 80

Author(s):

Andrea X. Silva ◽

Georg Jander ◽

Horacio Samaniego ◽

John S Ramsey ◽

Christian C. Figueroa

Keyword(s):

Insecticide Resistance ◽

Myzus Persicae ◽

Green Peach Aphid ◽

Resistance Mechanisms

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Insecticide Resistance Mechanisms in the Green Peach Aphid Myzus persicae (Hemiptera: Aphididae) II: Costs and Benefits

PLoS ONE ◽

10.1371/journal.pone.0036810 ◽

2012 ◽

Vol 7 (6) ◽

pp. e36810 ◽

Cited By ~ 27

Author(s):

Andrea X. Silva ◽

Leonardo D. Bacigalupe ◽

Manuela Luna-Rudloff ◽

Christian C. Figueroa

Keyword(s):

Insecticide Resistance ◽

Myzus Persicae ◽

Green Peach Aphid ◽

Resistance Mechanisms ◽

Costs And Benefits

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Monitoring insecticide resistance and diagnostics of resistance mechanisms in the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae) in China

Pesticide Biochemistry and Physiology ◽

10.1016/j.pestbp.2017.09.013 ◽

2017 ◽

Vol 143 ◽

pp. 39-47 ◽

Cited By ~ 20

Author(s):

Qiu-Ling Tang ◽

Kang-Sheng Ma ◽

You-Ming Hou ◽

Xi-Wu Gao

Keyword(s):

Insecticide Resistance ◽

Myzus Persicae ◽

Green Peach Aphid ◽

Resistance Mechanisms

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Clonal turnover of MACE-carrying peach-potato aphids (Myzus persicae (Sulzer), Homoptera: Aphididae) colonizing Scotland

Bulletin of Entomological Research ◽

10.1017/s0007485307005445 ◽

2007 ◽

Vol 98 (2) ◽

pp. 115-124 ◽

Cited By ~ 17

Author(s):

L. Kasprowicz ◽

G. Malloch ◽

S. Foster ◽

J. Pickup ◽

J. Zhan ◽

...

Keyword(s):

Insecticide Resistance ◽

Myzus Persicae ◽

Negative Selection ◽

Resistance Mechanism ◽

Resistance Mechanisms ◽

The Other ◽

Fitness Costs ◽

Field Samples ◽

Sexual Recombination ◽

Potato Aphids

AbstractPeach-potato aphids, Myzus persicae (Sulzer), collected in Scotland in the years 1995 and 2002–2004 were characterized using four microsatellite loci and three insecticide resistance mechanisms. From 868 samples, 14 multilocus genotypes were defined (designated clones A–N). Five of these (denoted A, B, H, M and N) carried modified acetylcholinesterase (MACE) resistance, the most recent resistance mechanism to have evolved in M. persicae. The current paper shows that the continued presence of MACE aphids is due to turnover, as clones A and B were replaced in field samples by clones H, M and N in later seasons. Thus, insecticide-resistant populations in Scotland can be attributed to multiple waves of rapid clone colonisations and not to the continued presence of stable resistant clones or mutation or sexual recombination in local populations. The MACE clones carried varying levels of the other insecticide resistance mechanisms, kdr and esterase. The presence of these mechanisms could alter the clones success in the field depending on insecticide spraying (positive selection) and resistance fitness costs (negative selection).

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Global patterns in genomic diversity underpinning the evolution of insecticide resistance in the aphid crop pest Myzus persicae

Communications Biology ◽

10.1038/s42003-021-02373-x ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Kumar Saurabh Singh ◽

Erick M. G. Cordeiro ◽

Bartlomiej J. Troczka ◽

Adam Pym ◽

Joanna Mackisack ◽

...

Keyword(s):

Host Plant ◽

Myzus Persicae ◽

Resistance Mechanisms ◽

Genomic Diversity ◽

Resistance Mutations ◽

Host Plant Adaptation ◽

Repeated Evolution ◽

Evolution Of Resistance ◽

Selective Forces ◽

Clonal Lines

AbstractThe aphid Myzus persicae is a destructive agricultural pest that displays an exceptional ability to develop resistance to both natural and synthetic insecticides. To investigate the evolution of resistance in this species we generated a chromosome-scale genome assembly and living panel of >110 fully sequenced globally sampled clonal lines. Our analyses reveal a remarkable diversity of resistance mutations segregating in global populations of M. persicae. We show that the emergence and spread of these mechanisms is influenced by host–plant associations, uncovering the widespread co‐option of a host-plant adaptation that also offers resistance against synthetic insecticides. We identify both the repeated evolution of independent resistance mutations at the same locus, and multiple instances of the evolution of novel resistance mechanisms against key insecticides. Our findings provide fundamental insights into the genomic responses of global insect populations to strong selective forces, and hold practical relevance for the control of pests and parasites.

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