Anti- and pro-mutagenic effects of silymarin in the ames bacterial reverse mutation assay

2009 ◽  
Vol 23 (10) ◽  
pp. 1378-1384 ◽  
Author(s):  
Sampath Kaleeswaran ◽  
Padmanabhan Sriram ◽  
Daivasigamani Prabhu ◽  
Chinnathambi ◽  
Vijayakumar ◽  
...  
Keyword(s):  
Reverse Mutation ◽  
Mutagenic Effects ◽  
Mutation Assay

scholarly journals Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro

Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 387-397
Author(s):  
Jasna Bosnjak-Neumüller ◽  
Ninoslav Djelic ◽  
Milena Radakovic ◽  
Stoimir Kolarevic ◽  
Dragana Mitic-Culafic ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Mammalian Cells ◽  
Ames Test ◽  
Human Lymphocytes ◽  
Genetic Material ◽  
Genotoxic Effects ◽  
Reverse Mutation ◽  
Mutagenic Effects ◽  
Mutation Assay

There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T3 concentrations was applied. The obtained results showed absence of genotoxic effects of T3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 ?M. We only observed cytotoxic effects in bacterial system at very high T3 concentrations (300 and 500 ?M). In conclusion, T3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T3 in mammalian cells.

scholarly journals Extracellular and intracellular anti-mutagenic effects of bile pigments in the Salmonella typhimurium reverse mutation assay

2013 ◽  
Vol 27 (1) ◽  
pp. 433-437 ◽  
Author(s):  
C. Mölzer ◽  
H. Huber ◽  
K. Diem ◽  
M. Wallner ◽  
A.C. Bulmer ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Bile Pigments ◽  
Reverse Mutation ◽  
Mutagenic Effects ◽  
Mutation Assay

In Vitro Cytotoxicity and Mutagenicity Evaluation of Dental Leucite Glass-Ceramics from Local High Grade Silica Sand

2015 ◽  
Vol 754-755 ◽  
pp. 979-984
Author(s):  
Siti Mazatul Azwa Bt Saiyed Mohd Nurddin ◽  
Malek B. Selamat
Keyword(s):  
Mtt Assay ◽  
Glass Ceramics ◽  
Mutagenic Effect ◽  
Negative Control ◽  
Silica Sand ◽  
High Grade ◽  
Reverse Mutation ◽  
Diphenyl Tetrazolium Bromide ◽  
Mutation Assay

The objective of the study was to determine the degree of biocompatibility of leucite glass-ceramics that have been produced from local high grade silica sand in terms of cytotoxicity and mutagenicity assays. In the present study, the cyctotoxicity and mutagenicity were studied using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay (MTT) and Ames Reverse Mutation. In the MTT assay, a dose response cytotoxicity of leucite sample was evaluated in L929 cells. The cells were treated with the concentrations of 6.25, 12.5, 25.0, 50.00, 100.00 and 200.00 mg/ml of the leucite sample for 24 hours. The cytotoxicity was determined by assessing the cell viability through the reduction of tetrazolium salts (MTT). The mutagenenicity of leucite sample was evaluated inS. typhiriumTA98. TA100, TA1535, TA1537 andE. coliWP2 in the Ames Reverse Mutation assay. Mutagenic effects were evaluated by comparing the mean number of revertant colonies of each extract concentraction with mean number of revertant colonies of the negative control. In results of MTT assay evaluated that the leucite did not show a cytotoxic effect at all concentrations under the condition of the study. Ames Reverse Mutation assay result proven that the leucite sample did not demonstrate a mutagenic effect under the condition of this study withSalmonella typhimuriumandEscherichia coli.

Comparison of a various modified mutagenicity assay and the standard bacterial reverse mutation assay

2007 ◽  
Vol 172 ◽  
pp. S168-S169
Author(s):  
Lee Eunmi ◽  
Jo Hyeon ◽  
Moon Hongsik ◽  
Kwak Byongsung
Keyword(s):  
Reverse Mutation ◽  
Mutagenicity Assay ◽  
Mutation Assay
Sign in / Sign up

Export Citation Format

Share Document