Quercetin promotes osteogenic differentiation and antioxidant responses of mouse bone mesenchymal stem cells through activation of the AMPK / SIRT1 signaling pathway

2021 ◽  
Author(s):  
Nan Wang ◽  
Luyao Wang ◽  
Jihao Yang ◽  
Zhihong Wang ◽  
Liangxing Cheng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Bone Mesenchymal Stem Cells ◽  
Antioxidant Responses

scholarly journals IL-17A Inhibits Osteogenic Differentiation of Bone Mesenchymal Stem Cells via Wnt Signaling Pathway

2017 ◽  
Vol 23 ◽  
pp. 4095-4101 ◽  
Author(s):  
Zhenguo Wang ◽  
Ying Jia ◽  
Fu Du ◽  
Min Chen ◽  
Xiuhua Dong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Wnt Signaling ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Bone Mesenchymal Stem Cells

MicroRNA-5106-based nanodelivery to enhance osteogenic differentiation and bone regeneration of bone mesenchymal stem cells through targeting of Gsk-3α

2021 ◽  
Author(s):  
Meng Yu ◽  
Bo Lei
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Intracellular Delivery ◽  
Bone Mesenchymal Stem Cells ◽  
Regeneration Of Bone

This work reports the intracellular delivery of miRNA-5106 into stem cells. The intracellular delivery could efficiently enhance the osteogenic differentiation and in vivo bone regeneration through the targeting the Gsk-3α signaling pathway.

scholarly journals Cornuside I promoted osteogenic differentiation of bone mesenchymal stem cells through PI3K/Akt signaling pathway

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Feng Gao ◽  
Sheng-Li Xia ◽  
Xiu-Hui Wang ◽  
Xiao-Xiao Zhou ◽  
Jun Wang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Differentially Expressed Genes ◽  
Akt Signaling ◽  
Differentially Expressed ◽  
Calcium Deposition ◽  
Akt Signaling Pathway ◽  
Bone Mesenchymal Stem Cells

Abstract Background Osteoporosis is a common disease closely associated with aging. In this study, we aimed to investigate the role of Cornuside I in promoting osteogenic differentiation of bone mesenchymal stem cells (BMSCs) and the potential mechanism. Methods BMSCs were isolated and treated with different concentrations of Cornuside I (0, 10, 30, 60 μM). Cell proliferation was analyzed by Cell Counting Kit-8 (CCK-8) assay. RNA sequencing was performed on the isolated BMSCs with control and Cornuside I treatment. Differentially expressed genes were obtained by the R software. Alkaline phosphatase (ALP) staining and Alizarin Red Staining (ARS) were performed to assess the osteogenic capacity of the NEO. qRT-PCR and western blot were used to detect the expression of osteoblast markers. Results Cornuside I treatment significantly improved BMSC proliferation. The optimal dose of Cornuside I was 30 μM (P < 0.05). Cornuside I dose dependently increased the ALP activity and calcium deposition than control group (P < 0.05). A total of 704 differentially expressed genes were identified between Cornuside I and normal BMSCs. Cornuside I significantly increased the PI3K and Akt expression. Moreover, the promotion effects of Cornuside I on osteogenic differentiation of BMSCs were partially blocked by PI3K/Akt inhibitor, LY294002. Conclusion Cornuside I plays a positive role in promoting osteogenic differentiation of BMSCs, which was related with activation of PI3K/Akt signaling pathway.

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