scholarly journals Microgels: SERS-Active-Charged Microgels for Size- and Charge-Selective Molecular Analysis of Complex Biological Samples (Small 40/2018)

Small ◽  
2018 ◽  
Vol 14 (40) ◽  
pp. 1870183
Author(s):  
Dong Jae Kim ◽  
Sung-Gyu Park ◽  
Dong-Ho Kim ◽  
Shin-Hyun Kim
Keyword(s):  
Molecular Analysis ◽  
Biological Samples

scholarly journals SERS‐Active‐Charged Microgels for Size‐ and Charge‐Selective Molecular Analysis of Complex Biological Samples

Small ◽  
2018 ◽  
Vol 14 (50) ◽  
pp. 1804655
Author(s):  
Dong Jae Kim ◽  
Sung‐Gyu Park ◽  
Dong‐Ho Kim ◽  
Shin‐Hyun Kim
Keyword(s):  
Molecular Analysis ◽  
Biological Samples

scholarly journals Identification of Serogroups Australis and Icterohaemorrhagiae in Two Dogs with a Severe Form of Acute Leptospirosis in Italy

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 351 ◽  
Author(s):  
Andrea Balboni ◽  
Silvia Zamagni ◽  
Cristina Bertasio ◽  
Maria Beatrice Boniotti ◽  
Roberta Troìa ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Molecular Analysis ◽  
Biological Samples ◽  
Clinical Studies ◽  
Clinical Presentation ◽  
Severe Form ◽  
Sequence Type ◽  
Serious Illness ◽  
Severe Clinical Presentation ◽  
Leptospira Spp

Leptospirosis is an infectious disease that causes serious illness in dogs. For this reason, epidemiological and clinical studies focusing on disease characterization are widely advocated. The aim of this study was to characterize the leptospires identified in dogs with confirmed symptomatic acute leptospirosis. Leptospira spp. DNA detected in urine, blood, or both samples from nine infected dogs was analyzed using the multi-locus sequence typing (MLST) technique. Leptospires from two dogs were successfully typed: one was identified as belonging to Sequence Type (ST) 17 and one to ST198, both within the L. interrogans species, serogroups Icterohaemorrhagiae and Australis, respectively. Based on the results of routine serologic tests, antibodies reactive toward these serogroups are commonly revealed in dogs in Italy. This study provides the first molecular analysis that identifies infecting Leptospira directly on DNA from biological samples of dogs, showing that serogroup Australis can lead to a severe clinical presentation of leptospirosis in infected dogs.

Spatially Controlled Molecular Analysis of Biological Samples Using Nanodroplet Arrays and Direct Droplet Aspiration

2020 ◽  
Vol 31 (2) ◽  
pp. 418-428 ◽  
Author(s):  
Marta Sans ◽  
Anna Krieger ◽  
Bryan R. Wygant ◽  
Kyana Y. Garza ◽  
C. Buddie Mullins ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Biological Samples

SERS-Active-Charged Microgels for Size- and Charge-Selective Molecular Analysis of Complex Biological Samples

Small ◽  
2018 ◽  
Vol 14 (40) ◽  
pp. 1802520 ◽  
Author(s):  
Dong Jae Kim ◽  
Sung-Gyu Park ◽  
Dong-Ho Kim ◽  
Shin-Hyun Kim
Keyword(s):  
Molecular Analysis ◽  
Biological Samples

scholarly journals Plasmonics-Nanofluidics Metamaterial: An Ultrasensitive Platform for Terahertz biosensing and Quantitative Measurement of Molecules

2020 ◽  
Author(s):  
Mingzhen Xie ◽  
Jinchun He ◽  
Yu Liu ◽  
Huiyan Tian ◽  
Xinyu Zhan ◽  
...  
Keyword(s):  
Refractive Index ◽  
Liquid Phase ◽  
Molecular Analysis ◽  
Biological Samples ◽  
Terahertz Spectroscopy ◽  
Split Ring Resonator ◽  
Thz Spectroscopy ◽  
Biological Sensing ◽  
Label Free ◽  
Molecular Fingerprints

Abstract Background: The terahertz spectroscopy has attracted enormous attention for label free chemical and biological sensing, due to the presence of molecular fingerprints and remote sensing capabilities. However, because of the strong absorption of THz waves in aqueous solutions, detecting liquid-phase biological samples by THz spectroscopy has encountered strong constraints. Results: Here, we demonstrate for the first time a rapid and label-free sensing for liquid-phase biological samples using THz spectroscopy based on microfluidic metasensors. The metasensors were integrated with microfluidic chip to precisely control the liquid sample thickness within 220 nanometer (MMS-N) and 50 micrometer (MMS-U). The metasensors include a periodic split ring resonator (SRR) with two resonant peaks in the THz range. The sensitivity(S) and corresponding figure of merit (FOM) of the two resonance peaks of MMS-N and MMS-U are analyzed with respect to different refractive index matching solution. Both the two resonance peaks of the metasensors are sensitive to the varies refractive index environment and high-frequency resonant of MMS-N shows the best sensitivity(~136GHz/RIU). In addition, the label-free biological sensing capability was conducted through qualitative and quantitative LDL and ox-LDL, where a shift of resonance frequency was observed as the LDL and ox-LDL concentration increased. Conclusions: The results indicated nanofluidic metasensor presented excellent sensing capability for liquid-phase molecular analysis by THz spectroscopy, and the proposed sensing system has the potentials to be developed as a rapid, label-free and highly sensitive detection tool for molecular analysis by THz sensing.

An Efficient Statistic for Determining the Diameter of Thin Sectioned Uniform Spheres from Measurements of Biological Samples

1974 ◽  
Vol 32 ◽  
pp. 114-115
Author(s):  
W. R. Schucany ◽  
G. H. Kelsoe ◽  
V. F. Allison
Keyword(s):  
Biological Samples ◽  
Traditional Method ◽  
Cell Types ◽  
Accurate Estimation ◽  
Morphological Identification ◽  
Sectioned Material ◽  
Maximal Diameter ◽  
Similar Cell

Accurate estimation of the size of spheroid organelles from thin sectioned material is often necessary, as uniquely homogenous populations of organelles such as vessicles, granules, or nuclei often are critically important in the morphological identification of similar cell types. However, the difficulty in obtaining accurate diameter measurements of thin sectioned organelles is well known. This difficulty is due to the extreme tenuity of the sectioned material as compared to the size of the intact organelle. In populations where low variance is suspected the traditional method of diameter estimation has been to measure literally hundreds of profiles and to describe the “largest” as representative of the “approximate maximal diameter”.

The Application of Ultra-Thin Sectioning Techniques to Non-Biological Samples

1968 ◽  
Vol 26 ◽  
pp. 332-333
Author(s):  
C. F. Oster
Keyword(s):  
Biological Sciences ◽  
Epoxy Resin ◽  
Cross Sections ◽  
Biological Samples ◽  
Industrial Applications ◽  
Photographic Film ◽  
Silver Particles ◽  
Thin Sectioning ◽  
Embedding Medium

Although ultra-thin sectioning techniques are widely used in the biological sciences, their applications are somewhat less popular but very useful in industrial applications. This presentation will review several specific applications where ultra-thin sectioning techniques have proven invaluable.The preparation of samples for sectioning usually involves embedding in an epoxy resin. Araldite 6005 Resin and Hardener are mixed so that the hardness of the embedding medium matches that of the sample to reduce any distortion of the sample during the sectioning process. No dehydration series are needed to prepare our usual samples for embedding, but some types require hardening and staining steps. The embedded samples are sectioned with either a prototype of a Porter-Blum Microtome or an LKB Ultrotome III. Both instruments are equipped with diamond knives.In the study of photographic film, the distribution of the developed silver particles through the layer is important to the image tone and/or scattering power. Also, the morphology of the developed silver is an important factor, and cross sections will show this structure.

Artefacts in the x-ray microanalysis of frozen-hydrated biological samples

1987 ◽  
Vol 45 ◽  
pp. 658-659
Author(s):  
Patrick Echlin
Keyword(s):  
Electron Scattering ◽  
Biological Samples ◽  
Fracture Face ◽  
Detailed Structure ◽  
X Ray ◽  
Morphological Appearance ◽  
The Poor ◽  
Freeze Dried

A number of papers have appeared recently which purport to have carried out x-ray microanalysis on fully frozen hydrated samples. It is important to establish reliable criteria to be certain that a sample is in a fully hydrated state. The morphological appearance of the sample is an obvious parameter because fully hydrated samples lack the detailed structure seen in their freeze dried counterparts. The electron scattering by ice within a frozen-hydrated section and from the surface of a frozen-hydrated fracture face obscures cellular detail. (Fig. 1G and 1H.) However, the morphological appearance alone can be quite deceptive for as Figures 1E and 1F show, parts of frozen-dried samples may also have the poor morphology normally associated with fully hydrated samples. It is only when one examines the x-ray spectra that an assurance can be given that the sample is fully hydrated.

Molecular analysis of neurotransmitter release

1998 ◽  
Vol 33 ◽  
pp. 29-41 ◽  
Author(s):  
Giampietro Schiavo ◽  
Gudrun Stenbeck
Keyword(s):  
Molecular Analysis ◽  
Neurotransmitter Release
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