scholarly journals Captopril Inhibits the Proliferation of Hematopoietic Stem and Progenitor Cells in Murine Long‐Term Bone Marrow Cultures

Stem Cells ◽  
1999 ◽  
Vol 17 (6) ◽  
pp. 339-344 ◽  
Author(s):  
John Eugenes Chisi ◽  
Joanna Wdzieczak‐Bakala ◽  
Josiane Thierry ◽  
Cecile V. Briscoe ◽  
Andrew C. Riches
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells ◽  
Bone Marrow Cultures ◽  
Marrow Cultures

scholarly journals Human osteoblasts support human hematopoietic progenitor cells in vitro bone marrow cultures

Blood ◽  
1996 ◽  
Vol 87 (2) ◽  
pp. 518-524 ◽  
Author(s):  
RS Taichman ◽  
MJ Reilly ◽  
SG Emerson
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Stem Cell Differentiation ◽  
Colony Stimulating Factor ◽  
Hematopoietic Stem ◽  
Human Osteoblasts ◽  
Bone Marrow Cultures ◽  
Stimulating Factor ◽  
Marrow Cultures

Hematopoietic stem cell differentiation occurs in direct proximity to osteoblasts within the bone marrow cavity. Despite this striking affiliation, surprisingly little is known about the precise cellular and molecular impact of osteoblasts on the bone marrow microenvironment. Recently, we showed that human osteoblasts produce a variety of cytokine mRNAs including granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and interleukin-6. We examined here the ability of osteoblasts to support the development of hematopoietic colonies from progenitors as well the ability to maintain long-term culture-initiating cells (LTC-IC) in vitro. Examination of the hematopoietic cells recovered after 2 weeks of culture showed that osteoblasts support the maintenance of immature hematopoietic phenotypes. In methylcellulose assays, osteoblasts stimulate the development of hematopoietic colonies to a level at least 10-fold over controls from progenitor cells. Using limiting dilutional bone marrow cultures, we observed an activity produced by osteoblasts resulting in an threefold to fourfold expansion of human LTC-IC and progenitor cells in vitro. Thus, the presence of hematopoietic stem cells in close proximity to endosteal surfaces in vivo may be due in part to a requirement for osteoblast-derived products.

scholarly journals Mechanism of Action of Diallyl Sulphide in Ameliorating the Hematopoietic Radiation Injury

2021 ◽  
Author(s):  
Omika Katoch ◽  
Mrinalini Tiwari ◽  
Namita Kalra ◽  
Paban K. Agrawala
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Proliferation And Differentiation ◽  
Stem And Progenitor Cells ◽  
Radiation Induced ◽  
Medicinal Properties ◽  
Marrow Cellularity

AbstractDiallyl sulphide (DAS), the pungent component of garlic, is known to have several medicinal properties and has recently been shown to have radiomitigative properties. The present study was performed to better understand its mode of action in rendering radiomitigation. Evaluation of the colonogenic ability of hematopoietic progenitor cells (HPCs) on methocult media, proliferation and differentiation of hematopoietic stem cells (HSCs), and transplantation of stem cells were performed. The supporting tissue of HSCs was also evaluated by examining the histology of bone marrow and in vitro colony-forming unit–fibroblast (CFU-F) count. Alterations in the levels of IL-5, IL-6 and COX-2 were studied as a function of radiation or DAS treatment. It was observed that an increase in proliferation and differentiation of hematopoietic stem and progenitor cells occurred by postirradiation DAS administration. It also resulted in increased circulating and bone marrow homing of transplanted stem cells. Enhancement in bone marrow cellularity, CFU-F count, and cytokine IL-5 level were also evident. All those actions of DAS that could possibly add to its radiomitigative potential and can be attributed to its HDAC inhibitory properties, as was observed by the reversal radiation induced increase in histone acetylation.

scholarly journals Current Developments in Mobilization of Hematopoietic Stem and Progenitor Cells and Their Interaction with Niches in Bone Marrow

2017 ◽  
Vol 44 (3) ◽  
pp. 151-164 ◽  
Author(s):  
Rudolf Richter ◽  
Wolfgang Forssmann ◽  
Reinhard Henschler
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

scholarly journals FGF7 supports hematopoietic stem and progenitor cells and niche-dependent myeloblastoma cells via autocrine action on bone marrow stromal cells in vitro

2013 ◽  
Vol 440 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Ruri Ishino ◽  
Kaori Minami ◽  
Satowa Tanaka ◽  
Mami Nagai ◽  
Keiji Matsui ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

Subpopulations of Stromal Cells from Long-term Human Bone Marrow Cultures: Ontogeny of Progenitor Cells and Expression of Growth Hormone Receptors

1997 ◽  
Vol 26 (1) ◽  
pp. 11-28 ◽  
Author(s):  
D. T. Lincoln ◽  
F. Sinowatz ◽  
S. Gabius ◽  
H. J. Gabius ◽  
L. Temmim ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Hormone ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Hormone Receptors ◽  
Human Bone ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

scholarly journals Hematopoietic stem and progenitor cells are present in healthy gingiva tissue

2021 ◽  
Vol 218 (4) ◽  
Author(s):  
Siddharth Krishnan ◽  
Kelly Wemyss ◽  
Ian E. Prise ◽  
Flora A. McClure ◽  
Conor O’Boyle ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Immune Responses ◽  
Progenitor Cells ◽  
Myeloid Cell ◽  
Extramedullary Hematopoiesis ◽  
Innate Immune ◽  
Innate Immune Cells ◽  
Hematopoietic Stem ◽  
Effector Cells ◽  
Stem And Progenitor Cells

Hematopoietic stem cells reside in the bone marrow, where they generate the effector cells that drive immune responses. However, in response to inflammation, some hematopoietic stem and progenitor cells (HSPCs) are recruited to tissue sites and undergo extramedullary hematopoiesis. Contrasting with this paradigm, here we show residence and differentiation of HSPCs in healthy gingiva, a key oral barrier in the absence of overt inflammation. We initially defined a population of gingiva monocytes that could be locally maintained; we subsequently identified not only monocyte progenitors but also diverse HSPCs within the gingiva that could give rise to multiple myeloid lineages. Gingiva HSPCs possessed similar differentiation potentials, reconstitution capabilities, and heterogeneity to bone marrow HSPCs. However, gingival HSPCs responded differently to inflammatory insults, responding to oral but not systemic inflammation. Combined, we highlight a novel pathway of myeloid cell development at a healthy barrier, defining a gingiva-specific HSPC network that supports generation of a proportion of the innate immune cells that police this barrier.

Sign in / Sign up

Export Citation Format

Share Document