scholarly journals Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells

Stem Cells ◽  
2019 ◽  
Vol 38 (3) ◽  
pp. 352-368 ◽  
Author(s):  
Wenbin Liang ◽  
Pengcheng Han ◽  
Elizabeth H. Kim ◽  
Jordan Mak ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Pacemaker Cell ◽  
Canonical Wnt Signaling ◽  
Cell Specification ◽  
Canonical Wnt

scholarly journals Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells

Blood ◽  
2008 ◽  
Vol 111 (1) ◽  
pp. 122-131 ◽  
Author(s):  
Petter S. Woll ◽  
Julie K. Morris ◽  
Matt S. Painschab ◽  
Rebecca K. Marcus ◽  
Aimee D. Kohn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Human Embryonic Stem Cells ◽  
Stromal Cells ◽  
Hematopoietic Cells ◽  
Embryonic Stem ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt

Human embryonic stem cells (hESCs) provide an important means to effectively study soluble and cell-bound mediators that regulate development of early blood and endothelial cells in a human model system. Here, several complementary methods are used to demonstrate canonical Wnt signaling is important for development of hESC-derived cells with both hematopoietic and endothelial potential. Analyses using both standard flow cy-tometry, as well the more detailed high-throughput image scanning flow cytometry, characterizes sequential development of distinct early developing CD34brightCD31+Flk1+ cells and a later population of CD34dimCD45+ cells. While the CD34brightCD31+Flk1+ have a more complex morphology and can develop into both endothelial cells and hematopoietic cells, the CD34dimCD45+ cells have a simpler morphology and give rise to only hematopoietic cells. Treatment with dickkopf1 to inhibit Wnt signaling results in a dramatic decrease in development of cells with hematoendothelial potential. In addition, activation of the canonical Wnt signaling pathway in hESCs by coculture with stromal cells that express Wnt1, but not use of noncanonical Wnt5-expressing stromal cells, results in an accelerated differentiation and higher percentage of CD34brightCD31+Flk1+ cells at earlier stages of differentiation. These studies effectively demonstrate the importance of canonical Wnt signaling to mediate development of early hematoendothelial progenitors during human development.

scholarly journals Canonical Wnt Signaling Induces a Primitive Endoderm Metastable State in Mouse Embryonic Stem Cells

Stem Cells ◽  
2013 ◽  
Vol 31 (4) ◽  
pp. 752-764 ◽  
Author(s):  
Feodor D. Price ◽  
Hang Yin ◽  
Andrew Jones ◽  
Wilfred van Ijcken ◽  
Frank Grosveld ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Metastable State ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Primitive Endoderm ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt

scholarly journals Continuous Antagonism by Dkk1 Counter Activates Canonical Wnt Signaling and Promotes Cardiomyocyte Differentiation of Embryonic Stem Cells

2012 ◽  
Vol 21 (1) ◽  
pp. 54-66 ◽  
Author(s):  
Meena Rai ◽  
Joel M. Walthall ◽  
Jianyong Hu ◽  
Antonis K. Hatzopoulos
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Embryonic Stem ◽  
Cardiomyocyte Differentiation ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt

scholarly journals Coordination of sonic hedgehog and Wnt signaling determines ventral and dorsal telencephalic neuron types from human embryonic stem cells

Development ◽  
2009 ◽  
Vol 136 (23) ◽  
pp. 4055-4063 ◽  
Author(s):  
X.-J. Li ◽  
X. Zhang ◽  
M. A. Johnson ◽  
Z.-B. Wang ◽  
T. LaVaute ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Sonic Hedgehog ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

scholarly journals Defining early lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/ -catenin, Activin/Nodal and BMP signaling

Development ◽  
2008 ◽  
Vol 135 (17) ◽  
pp. 2969-2979 ◽  
Author(s):  
T. Sumi ◽  
N. Tsuneyoshi ◽  
N. Nakatsuji ◽  
H. Suemori
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Bmp Signaling ◽  
Lineage Specification ◽  
Canonical Wnt

Differentiation of human embryonic stem cells into cone photoreceptors through simultaneous inhibition of BMP, TGFβ and Wnt signaling

Development ◽  
2015 ◽  
Vol 142 (19) ◽  
pp. 3294-3306 ◽  
Author(s):  
Shufeng Zhou ◽  
Anthony Flamier ◽  
Mohamed Abdouh ◽  
Nicolas Tétreault ◽  
Andrea Barabino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cone Photoreceptors ◽  
Simultaneous Inhibition

scholarly journals Importance of WNT-dependent signaling for derivation and maintenance of primed pluripotent bovine embryonic stem cells

2021 ◽  
Author(s):  
Yao Xiao ◽  
Thiago F Amaral ◽  
Pablo J Ross ◽  
Delia A Soto ◽  
Kenneth E Diffenderfer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Embryonic Stem ◽  
Janus Kinase ◽  
Inhibitory Protein ◽  
Stat3 Phosphorylation ◽  
Series Of Experiments ◽  
Canonical Wnt

Abstract The WNT signaling system plays an important but paradoxical role in regulation of pluripotency. In the cow, IWR-1, which inhibits canonical WNT activation and has WNT-independent actions, promotes derivation of primed pluripotent embryonic stem cells (ESC) from the blastocyst. Here we describe a series of experiments to determine whether derivation of ESC could be generated by replacing IWR-1 with other inhibitors of WNT signaling. Results confirm the importance of inhibition of canonical WNT signaling for establishment of pluripotent ESC in cattle and indicate that actions of IWR-1 can be mimicked by the WNT secretion inhibitor IWP2 but not by the tankyrase inhibitor XAV939 or WNT inhibitory protein dickopf 1. The role of janus kinase (JAK)-mediated signaling pathways for maintenance of pluripotency of ESC was also evaluated. Maintenance of pluripotency of ESC lines was blocked by a broad inhibitor of JAK even though cells did not express phosphorylated signal transducer and activator of transcription 3 (STAT3). Further studies with blastocysts indicated IWR-1 blocks activation of STAT3 phosphorylation. A likely explanation is that IWR-1 blocks differentiation of ESC into a pSTAT3+ lineage. In conclusion, results presented here indicated the importance of inhibition of WNT signaling for derivation of pluripotent bovine ESC (bESC), the role of JAK signaling for maintenance of pluripotency and the participation of IWR-1 in inhibition of activation of STAT3.

scholarly journals Noncanonical Wnt Signaling Orchestrates Early Developmental Events toward Hematopoietic Cell Fate from Human Embryonic Stem Cells

2009 ◽  
Vol 4 (3) ◽  
pp. 248-262 ◽  
Author(s):  
Kausalia Vijayaragavan ◽  
Eva Szabo ◽  
Marc Bossé ◽  
Veronica Ramos-Mejia ◽  
Randall T. Moon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Cell Fate ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Hematopoietic Cell ◽  
Early Developmental
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