scholarly journals Insights into the Sigma-1 receptor Chaperone's cellular functions: A microarray report

Synapse ◽  
2011 ◽  
Vol 66 (1) ◽  
pp. 42-51 ◽  
Author(s):  
Shang-Yi Tsai ◽  
Richard Kyle Rothman ◽  
Tsung-Ping Su
Keyword(s):  
Cellular Functions ◽  
Sigma 1 Receptor ◽  
Sigma 1

scholarly journals The effects of terminal tagging on homomeric interactions of the sigma 1 receptor

2019 ◽  
Author(s):  
Hideaki Yano ◽  
Leanne Liu ◽  
Sett Naing ◽  
Lei Shi
Keyword(s):  
Ligand Binding ◽  
Neurological Disorders ◽  
Transmembrane Segment ◽  
Cellular Functions ◽  
Migration Patterns ◽  
Terminal Ligand ◽  
Sigma 1 Receptor ◽  
C Terminus ◽  
Sigma 1 ◽  
Drug Treatments

AbstractThe sigma 1 receptor (σ1R) has been implicated in cancers, neurological disorders, and substance use disorders. Yet, its molecular and cellular functions have not been well-understood. Recent crystal structures of σ1R reveal a single N-terminal transmembrane segment and C-terminal ligand-binding domain, and a trimeric organization. Nevertheless, outstanding issues surrounding the functional or pharmacological relevance of σ1R oligomerization remain, such as the minimal protomeric unit and the differentially altered oligomerization states by different classes of ligands. Western blot (WB) assays have been widely used to investigate protein oligomerizations. However, the unique topology of σ1R renders several intertwined challenges in WB. Here we describe a WB protocol without temperature denaturization to study the ligand binding effects on the oligomerization state of σ1R. Using this approach, we observed unexpected ladder-like incremental migration pattern of σ1R, demonstrating preserved homomeric interactions in the detergent environment. We compared the migration patterns of intact σ1R construct and the C-terminally tagged σ1R constructs, and found similar trends in response to drug treatments. In contrast, N-terminally tagged σ1R constructs show opposite trends to that of the intact construct, suggesting distorted elicitation of the ligand binding effects on oligomerization. Together, our findings indicate that the N-terminus plays an important role in eliciting the impacts of bound ligands, whereas the C-terminus is amenable for modifications for biochemical studies.

Synthesis, in vivo evaluation and direct comparison with [18F]FPS of a novel sigma-1 receptor radiotracer [18F]2-Flouroethyl-[(4-cyanophenoxy)methyl]piperidine ([18F]WLS1.002)

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S597-S597
Author(s):  
Rikki N Waterhouse ◽  
Jun Zhao ◽  
Raymond C Chang ◽  
Patty Carambot
Keyword(s):  
In Vivo Evaluation ◽  
Sigma 1 Receptor ◽  
Sigma 1

[123I]TPCNE: A novel SPET tracer for the sigma-1 receptor binds irreversibly in humans in vivo

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S655-S655
Author(s):  
James M Stone ◽  
Erik Arstad ◽  
Kjell Erlandsson ◽  
Rikki N Waterhouse ◽  
Peter J Ell ◽  
...  
Keyword(s):  
Sigma 1 Receptor ◽  
Sigma 1

Sigma-1 receptor modulates methamphetamine regulation of dopamine transporter activity

2016 ◽  
Vol 4 (Suppl. 2) ◽  
pp. A4.4
Author(s):  
Habibeh Koghbouei
Keyword(s):  
Dopamine Transporter ◽  
Sigma 1 Receptor ◽  
Sigma 1

The Sigma-1 Receptor Ligands Chlorpromazine and Trifluoperazine Inhibit Na+ Transport in Frog Skin Epithelium

BIOPHYSICS ◽  
2020 ◽  
Vol 65 (5) ◽  
pp. 784-787
Author(s):  
A. V. Melnitskaya ◽  
Z. I. Krutetskaya ◽  
V. G. Antonov ◽  
N. I. Krutetskaya
Keyword(s):  
Frog Skin ◽  
Receptor Ligands ◽  
Na Transport ◽  
Sigma 1 Receptor ◽  
Skin Epithelium ◽  
Frog Skin Epithelium ◽  
Sigma 1

scholarly journals Sigma 1 Receptor, Cholesterol and Endoplasmic Reticulum Contact Sites

Contact ◽  
2021 ◽  
Vol 4 ◽  
pp. 251525642110265
Author(s):  
Vladimir Zhemkov ◽  
Jen Liou ◽  
Ilya Bezprozvanny
Keyword(s):  
Endoplasmic Reticulum ◽  
Protein Composition ◽  
Sigma 1 Receptor ◽  
Functional Roles ◽  
Membrane Contact Sites ◽  
Contact Sites ◽  
Sigma 1 ◽  
Membrane Contact ◽  
Organelle Communication ◽  
Cellular Organelles

Recent studies indicated potential importance of membrane contact sites (MCS) between the endoplasmic reticulum (ER) and other cellular organelles. These MCS have unique protein and lipid composition and serve as hubs for inter-organelle communication and signaling. Despite extensive investigation of MCS protein composition and functional roles, little is known about the process of MCS formation. In this perspective, we propose a hypothesis that MCS are formed not as a result of random interactions between membranes of ER and other organelles but on the basis of pre-existing cholesterol-enriched ER microdomains.

scholarly journals Sigma-1 Receptor Promotes Mitochondrial Bioenergetics by Orchestrating ER Ca2+ Leak during Early ER Stress

Metabolites ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 422
Author(s):  
Zhanat Koshenov ◽  
Furkan E. Oflaz ◽  
Martin Hirtl ◽  
Johannes Pilic ◽  
Olaf A. Bachkoenig ◽  
...  
Keyword(s):  
Er Stress ◽  
Energy Demand ◽  
Molecular Mechanisms ◽  
High Energy ◽  
Human Neuroblastoma Cell ◽  
Mitochondrial Bioenergetics ◽  
Dependent Manner ◽  
Human Neuroblastoma ◽  
Sigma 1 Receptor ◽  
Sigma 1

The endoplasmic reticulum (ER) is a complex, multifunctional organelle of eukaryotic cells and responsible for the trafficking and processing of nearly 30% of all human proteins. Any disturbance to these processes can cause ER stress, which initiates an adaptive mechanism called unfolded protein response (UPR) to restore ER functions and homeostasis. Mitochondrial ATP production is necessary to meet the high energy demand of the UPR, while the molecular mechanisms of ER to mitochondria crosstalk under such stress conditions remain mainly enigmatic. Thus, better understanding the regulation of mitochondrial bioenergetics during ER stress is essential to combat many pathologies involving ER stress, the UPR, and mitochondria. This article investigates the role of Sigma-1 Receptor (S1R), an ER chaperone, has in enhancing mitochondrial bioenergetics during early ER stress using human neuroblastoma cell lines. Our results show that inducing ER stress with tunicamycin, a known ER stressor, greatly enhances mitochondrial bioenergetics in a time- and S1R-dependent manner. This is achieved by enhanced ER Ca2+ leak directed towards mitochondria by S1R during the early phase of ER stress. Our data point to the importance of S1R in promoting mitochondrial bioenergetics and maintaining balanced H2O2 metabolism during early ER stress.

MicroRNA-297 promotes cardiomyocyte hypertrophy via targeting sigma-1 receptor

Life Sciences ◽  
2017 ◽  
Vol 175 ◽  
pp. 1-10 ◽  
Author(s):  
Qinxue Bao ◽  
Mingyue Zhao ◽  
Li Chen ◽  
Yu Wang ◽  
Siyuan Wu ◽  
...  
Keyword(s):  
Cardiomyocyte Hypertrophy ◽  
Sigma 1 Receptor ◽  
Sigma 1
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