scholarly journals P25.15: Magnetic cell sorting and flow cytometry for the detection of fetal nucleated erythroblasts in maternal circulation during the first trimester of pregnancy

2008 ◽  
Vol 32 (3) ◽  
pp. 402-402
Author(s):  
A. Daniilidis ◽  
M. Mavromichali ◽  
K. Kouzi ◽  
G. Koliakos ◽  
N. Tsagias ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
First Trimester ◽  
Magnetic Cell Sorting ◽  
Maternal Circulation ◽  
First Trimester Of Pregnancy

scholarly journals Human Placenta Exosomes: Biogenesis, Isolation, Composition, and Prospects for Use in Diagnostics

2021 ◽  
Vol 22 (4) ◽  
pp. 2158 ◽  
Author(s):  
Evgeniya E. Burkova ◽  
Sergey E. Sedykh ◽  
Georgy A. Nevinsky
Keyword(s):  
Fetal Development ◽  
Biochemical Composition ◽  
First Trimester ◽  
Maternal Blood ◽  
Target Cells ◽  
Normal Functioning ◽  
Maternal Circulation ◽  
Placental Dysfunction ◽  
Bioactive Proteins ◽  
First Trimester Of Pregnancy

Exosomes are 40–100 nm nanovesicles participating in intercellular communication and transferring various bioactive proteins, mRNAs, miRNAs, and lipids. During pregnancy, the placenta releases exosomes into the maternal circulation. Placental exosomes are detected in the maternal blood even in the first trimester of pregnancy and their numbers increase significantly by the end of pregnancy. Exosomes are necessary for the normal functioning of the placenta and fetal development. Effects of exosomes on target cells depend not only on their concentration but also on their intrinsic components. The biochemical composition of the placental exosomes may cause various complications of pregnancy. Some studies relate the changes in the composition of nanovesicles to placental dysfunction. Isolation of placental exosomes from the blood of pregnant women and the study of protein, lipid, and nucleic composition can lead to the development of methods for early diagnosis of pregnancy pathologies. This review describes the biogenesis of exosomes, methods of their isolation, analyzes their biochemical composition, and considers the prospects for using exosomes to diagnose pregnancy pathologies.

scholarly journals First-trimester NRBC Count in Maternal Circulation: Correlation with Doppler Ultrasound Studies

2005 ◽  
Vol 53 (3) ◽  
pp. 315-317
Author(s):  
Ariadni Mavrou ◽  
Aggeliki Kolialexi ◽  
Athena Souka ◽  
Athanasios Pilalis ◽  
Yannis Kavalakis ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Resistance ◽  
First Trimester ◽  
Maternal Circulation ◽  
Arterial Perfusion ◽  
Uterine Arteries ◽  
Nucleated Red Blood Cells ◽  
High Impedance ◽  
First Trimester Of Pregnancy ◽  
Doppler Indices

This study aimed to determine whether the number of nucleated red blood cells (NRBCs) in maternal circulation during the first trimester of pregnancy could identify pregnancies that will have an anomalous Doppler in the second trimester. A total of 85 blood samples were obtained at 11–14 weeks of gestation with mean uterine arterial perfusion index >1.6, as noted by Doppler ultrasonography. NRBCs were enriched by magnetic automated cell sorting using anti-CD71 and were stained with May/Grunwald/Giemsa. A total of 4.8 NRBCs (range 1–75) were identified in 68 cases. Follow-up scans at 22–24 weeks were available in 46 cases. In 39 women, blood flow in the uterine arteries normalized, whereas in seven, high resistance was noted. One woman in the high-resistance group developed preeclampsia (PET; four NRBCs) and another delivered an intrauterine growth restriction (IUGR) baby (75 NRBCs). The number of NRBCs in women whose Doppler indices later normalized and in those who continued to have increased impedance was similar. The study indicates that NRBC number in maternal circulation during the first trimester cannot be used to screen pregnancies at high risk for developing preeclampsia (PET)/IUGR. High-impedance blood flow in the uterine arteries in the first trimester may be due to an unfinished process of trophoblastic invasion, most likely to be completed successfully by 22–24 weeks.

scholarly journals Placenta-derived exosomes continuously increase in maternal circulation over the first trimester of pregnancy

2014 ◽  
Vol 12 (1) ◽  
pp. 204 ◽  
Author(s):  
Suchismita Sarker ◽  
Katherin Scholz-Romero ◽  
Alejandra Perez ◽  
Sebastian E Illanes ◽  
Murray D Mitchell ◽  
...  
Keyword(s):  
First Trimester ◽  
Maternal Circulation ◽  
First Trimester Of Pregnancy

scholarly journals Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells

2009 ◽  
Vol 10 (12) ◽  
pp. 928-932 ◽  
Author(s):  
Hang Yan ◽  
Chen-guang Ding ◽  
Pu-xun Tian ◽  
Guan-qun Ge ◽  
Zhan-kui Jin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
Function Analysis ◽  
Treg Cells ◽  
Magnetic Cell Sorting ◽  
And Function ◽  
Flow Cytometry Sorting

FRACTIONAL DETERMINATIONS OF URINARY 17-KETOSTEROIDS IN HYPEREMESIS GRAVIDARUM

1962 ◽  
Vol 41 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Pentti A. Järvinen ◽  
Sykkö Pesonen ◽  
Pirkko Väänänen
Keyword(s):  
Hyperemesis Gravidarum ◽  
First Trimester ◽  
Control Group ◽  
Before And After ◽  
Daily Urine ◽  
First Trimester Of Pregnancy ◽  
The Difference

ABSTRACT The fractional determination of 17-ketosteroids in the daily urine was performed in nine cases of hyperemesis gravidarum and in four control cases, in the first trimester of pregnancy both before and after corticotrophin administration. The excretion of total 17-KS is similar in the two groups. Only in the hyperemesis group does the excretion of total 17-KS increase significantly after corticotrophin administration. The fractional determination reveals no difference between the two groups of patients with regard to the values of the fractions U (unidentified 17-KS), A (androsterone) and Rest (11-oxygenated 17-KS). The excretion of dehydroepiandrosterone is significantly higher in the hyperemesis group than in the control group. The excretion of androstanolone seems to be lower in the hyperemesis group than in the control group, but the difference is not statistically significant. The differences in the correlation between dehydroepiandrosterone and androstanolone in the two groups is significant. The high excretion of dehydroepiandrosterone and low excretion of androstanolone in cases of hyperemesis gravidarum is a sign of adrenal dysfunction.

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