N-Linked Oligosaccharide of β-Amyloid Precursor Protein (βAPP) of C6 Glioma-Cells: Putative Regulatory Role in βAPP Processing

1995 ◽  
Vol 210 (3) ◽  
pp. 703-710 ◽  
Author(s):  
F. Saito ◽  
A. Tani ◽  
T. Miyatake ◽  
K. Yanagisawa
Keyword(s):  
Amyloid Precursor Protein ◽  
Glioma Cells ◽  
C6 Glioma ◽  
Precursor Protein ◽  
Regulatory Role ◽  
C6 Glioma Cells ◽  
Β Amyloid

scholarly journals Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin-binding neuroregulatory factors

FEBS Letters ◽  
2003 ◽  
Vol 557 (1-3) ◽  
pp. 233-238 ◽  
Author(s):  
Yuko Umehara ◽  
Shuhei Yamada ◽  
Shuji Nishimura ◽  
Junichi Shioi ◽  
Nikolaos K. Robakis ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Chondroitin Sulfate ◽  
Glioma Cells ◽  
C6 Glioma ◽  
Precursor Protein ◽  
C6 Glioma Cells ◽  
Heparin Binding

Genistein alleviates the mitochondria-targeted DNA damage induced by β-amyloid peptides 25–35 in C6 glioma cells

2013 ◽  
Vol 38 (7) ◽  
pp. 1315-1323 ◽  
Author(s):  
Wei-wei Ma ◽  
Cheng-cheng Hou ◽  
Xin Zhou ◽  
Huan-ling Yu ◽  
Yuan-di Xi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Amyloid Peptides ◽  
Β Amyloid

Production of the Alzheimer's β-amyloid peptide by C6 glioma cells

FEBS Letters ◽  
1993 ◽  
Vol 336 (2) ◽  
pp. 275-278 ◽  
Author(s):  
Esperanza Morato ◽  
Federico Mayor
Keyword(s):  
Glioma Cells ◽  
C6 Glioma ◽  
Amyloid Peptide ◽  
C6 Glioma Cells ◽  
Β Amyloid ◽  
Β Amyloid Peptide

Radiosensitivity of glioma to Gamma Knife treatment enhanced in vitro and in vivo by RNA interfering Ku70 that is mediated by a recombinant adenovirus

2010 ◽  
Vol 113 (Special_Supplement) ◽  
pp. 228-235 ◽  
Author(s):  
Qiang Jia ◽  
Yanhe Li ◽  
Desheng Xu ◽  
Zhenjiang Li ◽  
Zhiyuan Zhang ◽  
...  
Keyword(s):  
Western Blot ◽  
Gamma Knife ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells

Object The authors sought to evaluate modification of the radiation response of C6 glioma cells in vitro and in vivo by inhibiting the expression of Ku70. To do so they investigated the effect of gene transfer involving a recombinant replication-defective adenovirus containing Ku70 short hairpin RNA (Ad-Ku70shRNA) combined with Gamma Knife treatment (GKT). Methods First, Ad-Ku70shRNA was transfected into C6 glioma cells and the expression of Ku70 was measured using Western blot analysis. In vitro, phenotypical changes in C6 cells, including proliferation, cell cycle modification, invasion ability, and apoptosis were evaluated using the MTT (3′(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay, Western blot analysis, and cell flow cytometry. In vivo, parental C6 cells transfected with Ad-Ku70shRNA were implanted stereotactically into the right caudate nucleus in Sprague-Dawley rats. After GKS, apoptosis was analyzed using the TUNEL (terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling) method. The inhibitory effects on growth and invasion that were induced by expression of proliferating cell nuclear antigen and matrix metalloproteinase–9 were determined using immunohistochemical analyses. Results The expression of Ku70 was clearly inhibited in C6 cells after transfection with Ad-Ku70shRNA. In vitro following transfection, the C6 cells showed improved responses to GKT, including suppression of proliferation and invasion as well as an increased apoptosis index. In vivo following transfection of Ad-Ku70shRNA, the therapeutic efficacy of GKT in rats with C6 gliomas was greatly enhanced and survival times in these animals were prolonged. Conclusions Our data support the potential for downregulation of Ku70 expression in enhancing the radiosensitivity of gliomas. The findings of our study indicate that targeted gene therapy–mediated inactivation of Ku70 may represent a promising strategy in improving the radioresponsiveness of gliomas to GKT.

scholarly journals Transfected Go1 alpha inhibits the calcium dependence of beta-adrenergic stimulated cAMP accumulation in C6 glioma cells.

1993 ◽  
Vol 268 (12) ◽  
pp. 8980-8989
Author(s):  
N. Charpentier ◽  
L. Prézeau ◽  
J. Carrette ◽  
R. Bertorelli ◽  
G. Le Cam ◽  
...  
Keyword(s):  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Camp Accumulation ◽  
Beta Adrenergic ◽  
Calcium Dependence
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