Reciprocal Gene Expression of Osteoclastogenesis Inhibitory Factor and Osteoclast Differentiation Factor Regulates Osteoclast Formation

1999 ◽  
Vol 257 (3) ◽  
pp. 719-723 ◽  
Author(s):  
Masazumi Nagai ◽  
Nobuko Sato
Keyword(s):  
Gene Expression ◽  
Osteoclast Differentiation ◽  
Osteoclast Formation ◽  
Inhibitory Factor ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor

scholarly journals Gene Expression of Osteoclast Differentiation Factor Is Induced by Lipopolysaccharide in Mouse Osteoblasts Via Toll-Like Receptors

2001 ◽  
Vol 166 (5) ◽  
pp. 3574-3579 ◽  
Author(s):  
Takeshi Kikuchi ◽  
Tetsuya Matsuguchi ◽  
Naotake Tsuboi ◽  
Akio Mitani ◽  
Shigehisa Tanaka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Osteoclast Differentiation ◽  
Toll Like Receptors ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor

Mechanical strain inhibits expression of osteoclast differentiation factor by murine stromal cells

2000 ◽  
Vol 278 (6) ◽  
pp. C1126-C1132 ◽  
Author(s):  
Janet Rubin ◽  
Tamara Murphy ◽  
Mark S. Nanes ◽  
Xian Fan
Keyword(s):  
Stromal Cells ◽  
Mechanical Strain ◽  
Osteoclast Differentiation ◽  
Osteoclast Formation ◽  
Thymidine Uptake ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor ◽  
Membrane Bound ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony

Normal dynamic loading prevents bone resorption; however, the means whereby biophysical factors reduce osteoclast activity are not understood. We show here that mechanical strain (2% at 10 cycles per minute) applied to murine marrow cultures reduced 1,25(OH)2D3-stimulated osteoclast formation by 50%. This was preceded by decreased expression of osteoclast differentiation factor (ODF/TRANCE). RT-PCR for ODF/TRANCE revealed that ODF/TRANCE mRNA in strained cultures was 59 ± 3% of that seen in control cultures. No significant effects on total cell count, thymidine uptake, or alkaline phosphatase activity were induced by strain. To isolate the cell targeted by strain, primary stromal cells were cultured from marrow. Mechanical strain also reduced mRNA for ODF/TRANCE to 60% that of control in these cells. In contrast, mRNA for membrane-bound macrophage colony-stimulating factor was not significantly affected. Soluble ODF (∼2 ng/ml) was able to reverse the effect of strain, returning osteoclast numbers to control. Because osteoclast formation is dependent upon ODF/TRANCE expression, strain-induced reductions in this factor may contribute to the accompanying reduction in osteoclastogenesis.

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