Role of Ascorbic Acid in the Osteoclast Formation: Induction of Osteoclast Differentiation Factor with Formation of the Extracellular Collagen Matrix*

Normal dynamic loading prevents bone resorption; however, the means whereby biophysical factors reduce osteoclast activity are not understood. We show here that mechanical strain (2% at 10 cycles per minute) applied to murine marrow cultures reduced 1,25(OH)2D3-stimulated osteoclast formation by 50%. This was preceded by decreased expression of osteoclast differentiation factor (ODF/TRANCE). RT-PCR for ODF/TRANCE revealed that ODF/TRANCE mRNA in strained cultures was 59 ± 3% of that seen in control cultures. No significant effects on total cell count, thymidine uptake, or alkaline phosphatase activity were induced by strain. To isolate the cell targeted by strain, primary stromal cells were cultured from marrow. Mechanical strain also reduced mRNA for ODF/TRANCE to 60% that of control in these cells. In contrast, mRNA for membrane-bound macrophage colony-stimulating factor was not significantly affected. Soluble ODF (∼2 ng/ml) was able to reverse the effect of strain, returning osteoclast numbers to control. Because osteoclast formation is dependent upon ODF/TRANCE expression, strain-induced reductions in this factor may contribute to the accompanying reduction in osteoclastogenesis.

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Reciprocal Gene Expression of Osteoclastogenesis Inhibitory Factor and Osteoclast Differentiation Factor Regulates Osteoclast Formation

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1999.0524 ◽

1999 ◽

Vol 257 (3) ◽

pp. 719-723 ◽

Cited By ~ 82

Author(s):

Masazumi Nagai ◽

Nobuko Sato

Keyword(s):

Gene Expression ◽

Osteoclast Differentiation ◽

Osteoclast Formation ◽

Inhibitory Factor ◽

Differentiation Factor ◽

Osteoclast Differentiation Factor

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Basic Fibroblast Growth Factor Induces Osteoclast Formation by Reciprocally Regulating the Production of Osteoclast Differentiation Factor and Osteoclastogenesis Inhibitory Factor in Mouse Osteoblastic Cells

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1999.1601 ◽

1999 ◽

Vol 265 (1) ◽

pp. 158-163 ◽

Cited By ~ 45

Author(s):

Nobuaki Nakagawa ◽

Hisataka Yasuda ◽

Kazuki Yano ◽

Shin-ichi Mochizuki ◽

Naoki Kobayashi ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Osteoclast Differentiation ◽

Osteoclast Formation ◽

Inhibitory Factor ◽

Osteoblastic Cells ◽

Fibroblast Growth ◽

Differentiation Factor ◽

Osteoclast Differentiation Factor

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Inhibition of osteoclast formation by 3-methylcholanthrene, a ligand for arylhydrocarbon receptor: suppression of osteoclast differentiation factor in osteogenic cells

Biochemical Pharmacology ◽

10.1016/j.bcp.2003.08.038 ◽

2004 ◽

Vol 67 (1) ◽

pp. 119-127 ◽

Cited By ~ 27

Author(s):

M Naruse ◽

E Otsuka ◽

M Naruse ◽

Y Ishihara ◽

S Miyagawa-Tomita ◽

...

Keyword(s):

Osteoclast Differentiation ◽

Osteoclast Formation ◽

Osteogenic Cells ◽

Differentiation Factor ◽

Osteoclast Differentiation Factor ◽

Arylhydrocarbon Receptor

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Bone Morphogenetic Protein-2 Enhances Osteoclast Formation Mediated by Interleukin-1α through Upregulation of Osteoclast Differentiation Factor and Cyclooxygenase-2

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1999.0715 ◽

1999 ◽

Vol 259 (1) ◽

pp. 97-102 ◽

Cited By ~ 84

Author(s):

Masanori Koide ◽

Yoshiyuki Murase ◽

Kenji Yamato ◽

Toshihide Noguchi ◽

Nobuo Okahashi ◽

...

Keyword(s):

Bone Morphogenetic Protein ◽

Osteoclast Differentiation ◽

Osteoclast Formation ◽

Cyclooxygenase 2 ◽

Bone Morphogenetic Protein 2 ◽

Differentiation Factor ◽

Morphogenetic Protein ◽

Osteoclast Differentiation Factor ◽

Interleukin 1Α

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Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice

Laboratory Animal Research ◽

10.1186/s42826-020-00080-y ◽

2021 ◽

Vol 37 (1) ◽

Author(s):

Ae Ri Kim ◽

Yun Kyong Lim ◽

Joong-Ki Kook ◽

Eun-Jung Bak ◽

Yun-Jung Yoo

Keyword(s):

Flow Cytometry ◽

Porphyromonas Gingivalis ◽

Osteoclast Differentiation ◽

Fusobacterium Nucleatum ◽

Pathological Changes ◽

Air Pouch ◽

E Coli ◽

Differentiation Factor ◽

Osteoclast Differentiation Factor

AbstractIncreases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not been clarified yet. We evaluated numerical changes of RANKL-expressing neutrophils in air pouches of mice injected with LPSs of Fusobacterium nucleatum and Porphyromonas gingivalis. Mice with air pouches were assigned into saline (C)-, E. coli LPS- (Ec LPS)-, F. nucleatum LPS (Fn LPS)-, P. gingivalis LPS (Pg LPS)-, and Fn LPS and Pg LPS (Fn + Pg LPS)-injected groups. CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils in blood and air pouch exudates were determined by flow cytometry. In blood, compared to the C group, the Fn LPS group showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils whereas the Pg LPS group showed no significant differences. These increases in the Fn LPS group were not different to those in the Ec LPS group. In exudates, Fn LPS and Pg LPS groups showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils compared to the C group. Increased levels in the Fn LPS group were not different to those in the Ec LPS group, but Pg LPS group was lower than those in the Ec LPS group. In blood and exudates, the Fn + Pg LPS group showed no difference in levels of these neutrophils compared to the Ec LPS group. LPSs of F. nucleatum and P. gingivalis increased RANKL-expressing neutrophils although the degrees of increases were different. These suggest that periodontopathogen LPS can act as a stimulant to increase RANKL-expressing neutrophils.

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