Molecular Analyses of Old World Leishmania RAPD Markers and Development of a PCR Assay Selective for Parasites of the L. donovani Species Complex

2001 ◽  
Vol 98 (2) ◽  
pp. 90-99 ◽  
Author(s):  
R. Hanafi ◽  
M. Barhoumi ◽  
S.B. Ali ◽  
I. Guizani
Keyword(s):  
Species Complex ◽  
Rapd Markers ◽  
Pcr Assay ◽  
Molecular Analyses ◽  
Old World

Morphological and molecular characterisation of Xiphinema mazandaranense n. sp. (Dorylaimida: Longidoridae), a new member of the Xiphinema pyrenaicum species complex

Nematology ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Majid Pedram ◽  
Ebrahim Pourjam ◽  
Robert T. Robbins ◽  
Weimin Ye ◽  
Mohammad Reza Atighi ◽  
...  
Keyword(s):  
New Species ◽  
Species Complex ◽  
Small Subunit ◽  
Molecular Characterisation ◽  
Molecular Analyses ◽  
Small Subunit Rdna ◽  
Group 6 ◽  
Close Relationship ◽  
Juvenile Stages ◽  
Female Genital

Xiphinema mazandaranense n. sp. is described and illustrated. The new species belongs to morphospecies group 6, which is characterised by having two equally developed female genital branches that have spines in the uteri and a short, rounded tail. The new species is characterised by having spines in the tubular portion of the uterus, body 3.7-5.2 mm long, odontostyle 163-173 μm long, odontophore 96-100 μm long, body 62-80 μm diam., rounded tail, four juvenile stages, males rare with three ventromedian supplements next to the adcloacal pair and spicules 85 μm long. The polytomous identification codes of the new species are: A4-B3-C7b-D6-E456-F45-G4-H2-I23-J7b-K2-L1. The new species appears closely related to the members of X. pyrenaicum group which are characterised by a rounded tail with or without an inconspicuous projecting bulge and a uterus devoid of Z-differentiation but showing spiniform structures. The new species differs from members of the X. pyrenaicum group, which includes the recently described X. iranicum, by a more rounded tail without any projection. Beside morphological and morphometric data, molecular analyses of the near-full-length small subunit rDNA gene (SSU) placed the new species in close relationship with some species belonging to Xiphinema morphospecies group 6 and further separated this species from the X. pyrenaicum complex.

scholarly journals Deconstructing a Species-Complex: Geometric Morphometric and Molecular Analyses Define Species in the Western Rattlesnake (Crotalus viridis)

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0146166 ◽  
Author(s):  
Mark A. Davis ◽  
Marlis R. Douglas ◽  
Michael L. Collyer ◽  
Michael E. Douglas
Keyword(s):  
Species Complex ◽  
Geometric Morphometric ◽  
Molecular Analyses ◽  
Crotalus Viridis

Characterisation of the dimorphic Deladenus beddingi n. sp. and its associated woodwasp and fungus

Nematology ◽  
2018 ◽  
Vol 20 (10) ◽  
pp. 939-955 ◽  
Author(s):  
E. Erin Morris ◽  
S. Patricia Stock ◽  
Louela A. Castrillo ◽  
David W. Williams ◽  
Ann E. Hajek
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Relationships ◽  
Species Complex ◽  
Nematode Species ◽  
Evolutionary Relationships ◽  
Morphometric Traits ◽  
Molecular Analyses ◽  
Dimorphic Species ◽  
Multilocus Sequencing

Summary A new dimorphic species of Deladenus isolated from Sirex californicus from Washington, USA, is described as D. beddingi n. sp. Evolutionary relationships of the new species with other Deladenus species were assessed using multilocus sequencing. Phylogenetic relationships derived from analyses of mtCO1 and ITS showed D. beddingi n. sp. to be genetically distinct from other North American Deladenus parasitising Sirex. Molecular analyses indicated that D. beddingi n. sp. is a member of the D. siricidicola species complex, which also includes undescribed native Deladenus from Sirex cyaneus and S. nitidus, and D. siricidicola from S. noctilio. Mycophagous adults were characterised by the position of the excretory pore, which was located 32 (22-52) and 48 (38-69) μm anterior to the hemizonid in mycophagous females and males, respectively. Typologically, the new species is most similar to D. siricidicola, D. proximus and D. nitobei, but can be distinguished from these species by several morphometric traits, including the value of ratios a, b, c of the mycophagous females and males, ratio b of the infective females, and the morphology of the tail of the mycophagous females, which is narrow and gradually tapering. This novel nematode species feeds on the fungus Amylostereum chailletii during its mycophagous phase. Experimental results showed very little reproduction by D. beddingi n. sp. when feeding on A. areolatum compared to robust reproduction when feeding on A. chailletii.

scholarly journals Novel Simian Homologues of Epstein-Barr Virus

2003 ◽  
Vol 77 (19) ◽  
pp. 10695-10699 ◽  
Author(s):  
Bernhard Ehlers ◽  
Andreas Ochs ◽  
Fabian Leendertz ◽  
Michael Goltz ◽  
Christophe Boesch ◽  
...  
Keyword(s):  
Type Species ◽  
Epstein Barr Virus ◽  
New World ◽  
New World Primates ◽  
Pcr Assay ◽  
Old World ◽  
Barr Virus ◽  
World Primate ◽  
Epstein Barr

ABSTRACT Thirty different lymphocryptoviruses (LCV), 26 of them novel, were detected in primates by a panherpesvirus PCR assay. Nineteen LCV from chimpanzees, bonobos, gorillas, and other Old World primates were closely related to Epstein-Barr virus (EBV), the type species of the genus Lymphocryptovirus. Seven LCV originating from New World primates were related to callitrichine herpesvirus 3 (CalHV-3), the first recognized New World LCV. Importantly, a second LCV from gorillas and three LCV from orangutans and gibbons were only distantly related to EBV and CalHV-3. They were tentatively assigned to a novel genogroup of Old World primate LCV. The work described in the paper may also help identify an as yet unknown human LCV.

scholarly journals Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Mahmoud Nateghi Rostami ◽  
Fatemeh Darzi ◽  
Mahin Farahmand ◽  
Mohsen Aghaei ◽  
Parviz Parvizi
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Species ◽  
Pcr Assay ◽  
Old World

New molecular phylogeny of Lucinidae: increased taxon base with focus on tropical Western Atlantic species (Mollusca: Bivalvia)

Zootaxa ◽  
2016 ◽  
Vol 4196 (3) ◽  
pp. 381 ◽  
Author(s):  
JOHN D. TAYLOR ◽  
EMILY A. GLOVER ◽  
LISA SMITH ◽  
CHIHO IKEBE ◽  
SUZANNE T. WILLIAMS
Keyword(s):  
Molecular Phylogeny ◽  
Species Complex ◽  
Single Species ◽  
Abundant Species ◽  
28S Rrna ◽  
Western Atlantic ◽  
Molecular Analyses ◽  
West Pacific ◽  
Phylogenetic Framework ◽  
A New Species

A new molecular phylogeny of the Lucinidae using 18S and 28S rRNA and cytochrome b genes includes many species from the tropical Western Atlantic as well as additional taxa from the Indo-West Pacific. This study provides a phylogenetic framework for a new taxonomy of tropical Western Atlantic lucinids. The analysis confirmed five major clades—Pegophyseminae, Leucosphaerinae, Myrteinae, Codakiinae and Lucininae, with Monitilorinae and Fimbriinae represented by single species. The Leucosphaerinae are expanded and include Callucina winckworthi and the W. Atlantic Myrtina pristiphora that groups with several Indo-West Pacific Myrtina species. Within the Codakiinae two abundant species of Ctena from the Western Atlantic with similar shells are discriminated as C. orbiculata and C. imbricatula, while in the Indo-West Pacific Ctena bella is a probable species complex. The Lucininae is the most species rich and disparate subfamily with several subclades apparent. Three species of Lucina are recognized in the W. Atlantic L. aurantia, L. pensylvanica and L. roquesana. Pleurolucina groups near to Cavilinga and Lucina, while Lucinisca muricata is more closely related to the E. Pacific L. fenestrata than to the Atlantic L. nassula. A new species of Parvilucina is identified from molecular analyses having been confounded with Parvilucina pectinata but differs in ligament structure. Also, the former Parvilucina clenchi is more distant and assigned to Guyanella. 

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