Nuclear Magnetic Resonance Comparison of the Binding Sites of Mithramycin and Chromomycin on the Self-complementary Oligonucleotide d(ACCCGGGT)2

1993 ◽  
Vol 231 (3) ◽  
pp. 753-767 ◽  
Author(s):  
Max A. Keniry ◽  
Debra L. Banville ◽  
Peta M. Simmonds ◽  
Richard Shafer
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Binding Sites ◽  
The Self ◽  
Complementary Oligonucleotide ◽  
Nuclear Magnetic

A Nuclear Magnetic Resonance Study of the Metal Binding Sites in Bacitracin

1975 ◽  
Vol 53 (12) ◽  
pp. 1250-1254 ◽  
Author(s):  
Roderick E. Wasylishen ◽  
Moira R. Graham
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Metal Binding ◽  
Binding Sites ◽  
Imidazole Ring ◽  
Nuclear Magnetic Resonance Study ◽  
Resonance Spectroscopy ◽  
Metal Binding Sites ◽  
Ph Values ◽  
Nuclear Magnetic

Carbon-13 nuclear magnetic resonance spectroscopy has been used to identify sites in bacitracin which bind Cu2+ and Mn2+. Results are presented which implicate the free carboxyl groups of the aspartic and glutamic acid residues and the imidazole ring of the histidine residue as metal complexation sites between pH 6 and 8. Evidence is presented which also indicates that the thiazoline ring of bacitracin binds Mn2+. Bacitracin does not bind Cu2+ or Mn2+ at pH values of 2.5 or less.

scholarly journals A Fast Self-Learning Subspace Reconstruction Method for Non-Uniformly Sampled Nuclear Magnetic Resonance Spectroscopy

2020 ◽  
Vol 10 (11) ◽  
pp. 3939 ◽  
Author(s):  
Zhangren Tu ◽  
Huiting Liu ◽  
Jiaying Zhan ◽  
Di Guo
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
The Self ◽  
Resonance Spectroscopy ◽  
Reconstruction Method ◽  
Subspace Method ◽  
High Quality ◽  
Self Learning ◽  
Nuclear Magnetic

Multidimensional nuclear magnetic resonance (NMR) spectroscopy is one of the most crucial detection tools for molecular structure analysis and has been widely used in biomedicine and chemistry. However, the development of NMR spectroscopy is hampered by long data collection time. Non-uniform sampling empowers rapid signal acquisition by collecting a small subset of data. Since the sampling rate is lower than that of the Nyquist sampling ratio, undersampling artifacts arise in reconstructed spectra. To obtain a high-quality spectrum, it is necessary to apply reasonable prior constraints in spectrum reconstruction models. The self-learning subspace method has been shown to possess superior advantages than that of the state-of-the-art low-rank Hankel matrix method when adopting high acceleration in data sampling. However, the self-learning subspace method is time-consuming due to the singular value decomposition in iterations. In this paper, we propose a fast self-learning subspace method to enable fast and high-quality reconstructions. Aided by parallel computing, the experiment results show that the proposed method can reconstruct high-fidelity spectra but spend less than 10% of the time required by the non-parallel self-learning subspace method.

Characterization of water status in primed seeds of tomato (Lycopersicon esculentum Mill.) by sorption properties and NMR relaxation times

2005 ◽  
Vol 15 (2) ◽  
pp. 99-111 ◽  
Author(s):  
Shantha Nagarajan ◽  
V.K. Pandita ◽  
D.K. Joshi ◽  
J.P. Sinha ◽  
B.S. Modi
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Water Content ◽  
Binding Sites ◽  
Nmr Relaxation ◽  
Sorption Properties ◽  
Hydration Water ◽  
The Third ◽  
Germination Process ◽  
Nuclear Magnetic

The enhanced laboratory and field emergence characteristics of osmo- and halo-primed tomato seeds (cv. Pusa Ruby) were related to changes in hydration–dehydration kinetics, modified sorption properties and nuclear magnetic resonance (NMR) relaxation behaviour of humidified and imbibed seeds. Water sorption isotherms were constructed for primed and unprimed seeds by equilibrating to different water activities (aw) at 25°C. Analysis of the isotherms by the D'Arcy–Watt equation revealed that priming reduced the number of strong binding sites and the associated water content, and increased significantly the number of weak binding sites and the associated water content. This redistribution of water, which increased the availability of seed water, may be the reason for the higher speed of germination of primed seeds. The changes in transverse relaxation time (T2) of seed water and its components, measuredin vivousing nuclear magnetic resonance spectroscopy, showed interesting differences between primed and unprimed seeds. With an increase in humidification time, the T2of primed seeds could be resolved into three components with varying mobilities, while the control seeds had only two components until 10 d of humidification. During imbibition, the third component appeared after 2 and 6 h in primed and control seeds, respectively. This component disappeared after the germination process started in all treatments. The third fraction, with very low molecular mobility, which accounted for about 40% of the proton population, was assigned to hydration water of macromolecules. Hence, we propose that better performance of primed seeds may be attributed to the modifications of seed water-binding properties and reorganization of seed water during imbibition, so as to increase the macromolecular hydration water required for various metabolic activities related to the germination process.

Sign in / Sign up

Export Citation Format

Share Document