Cell Cycle-related Changes in the Voltage-gated Ca2+Currents in Cultured Newborn Rat Ventricular Myocytes

1998 ◽  
Vol 30 (6) ◽  
pp. 1095-1103 ◽  
Author(s):  
Weinong Guo ◽  
Kaichiro Kamiya ◽  
Itsuo Kodama ◽  
Junji Toyama
Keyword(s):  
Cell Cycle ◽  
Ventricular Myocytes ◽  
Newborn Rat ◽  
Voltage Gated ◽  
Rat Ventricular Myocytes

scholarly journals Correction to: Inhibitory effects of aloperine on voltage-gated Na+ channels in rat ventricular myocytes

2021 ◽  
Author(s):  
Meng-ting Li ◽  
Ya-ya Du ◽  
Fei Zhong ◽  
Jie-ru Wang ◽  
You-wei Gu ◽  
...  
Keyword(s):  
Ventricular Myocytes ◽  
Na Channels ◽  
Inhibitory Effects ◽  
Voltage Gated ◽  
Rat Ventricular Myocytes

Regulation of Cardiac Kv1.5 K+Channel Expression by Cardiac Fibroblasts and Mechanical Load in Cultured Newborn Rat Ventricular Myocytes

1998 ◽  
Vol 30 (1) ◽  
pp. 157-166 ◽  
Author(s):  
Weinong Guo ◽  
Kaichiro Kamiya ◽  
Kenji Kada ◽  
Itsuo Kodama ◽  
Junji Toyama
Keyword(s):  
Ventricular Myocytes ◽  
Mechanical Load ◽  
Cardiac Fibroblasts ◽  
K Channel ◽  
Newborn Rat ◽  
Rat Ventricular Myocytes ◽  
Channel Expression

Inhibitory effects of aloperine on voltage-gated Na+ channels in rat ventricular myocytes

2021 ◽  
Author(s):  
Meng-ting Li ◽  
Ya-ya Du ◽  
Fei Zhong ◽  
Jie-ru Wang ◽  
You-wei Gu ◽  
...  
Keyword(s):  
Ventricular Myocytes ◽  
Na Channels ◽  
Inhibitory Effects ◽  
Voltage Gated ◽  
Rat Ventricular Myocytes

scholarly journals Developmental analysis reveals mismatches in the expression of K+ channel alpha subunits and voltage-gated K+ channel currents in rat ventricular myocytes.

1996 ◽  
Vol 108 (5) ◽  
pp. 405-419 ◽  
Author(s):  
H Xu ◽  
J E Dixon ◽  
D M Barry ◽  
J S Trimmer ◽  
J P Merlie ◽  
...  
Keyword(s):  
Ventricular Myocytes ◽  
Developmental Expression ◽  
K Channel ◽  
Mrna Levels ◽  
K Channels ◽  
Voltage Gated ◽  
Rat Ventricular Myocytes ◽  
Adult Rat ◽  
Alpha Subunits ◽  
Channel Currents

In the experiments here, the developmental expression of the functional Ca(2+)-independent, depolarization-activated K+ channel currents, Ito and IK, and of the voltage-gated K+ channel (Kv) alpha subunits, Kv1.2, Kv1.4, Kv1.5, Kv2.1, and Kv4.2 in rat ventricular myocytes were examined quantitatively. Using the whole-cell patch clamp recording method, the properties and the densities of Ito and IK in ventricular myocytes isolated from postnatal day 5 (P5), 10 (P10), 15 (P15), 20 (P20), 25 (P25), 30 (P30), and adult (8-12 wk) rats were characterized and compared. These experiments revealed that mean Ito densities increase fourfold between birth and P30, whereas IK densities vary only slightly. Neither the time- nor the voltage-dependent properties of the currents vary measurably, suggesting that the subunits underlying functional Ito and IK channels are the same throughout postnatal development. In parallel experiments, the developmental expression of each of the voltage-gated K+ channel alpha subunits, Kv1.2, Kv1.4, Kv1.5, Kv2.1, and Kv4.2, was examined quantitatively at the mRNA and protein levels using subunit-specific probes. RNase protection assays revealed that Kv1.4 message levels are high at birth, increase between P0 and P10, and subsequently decrease to very low levels in adult rat ventricles. The decrease in message is accompanied by a marked reduction in Kv1.4 protein, consistent with our previous suggestion that Kv1.4 does not contribute to the formation of functional K+ channels in adult rat ventricular myocytes. In contrast to Kv1.4, the mRNA levels of Kv1.2, Kv1.5, Kv2.1, and Kv4.2 increase (three- to five-fold) between birth and adult. Western analyses, however, revealed that the expression patterns of these subunits proteins vary in distinct ways: Kv1.2 and Kv4.2, for example, increase between P5 and adult, whereas Kv1.5 remains constant and Kv2.1 decreases. Throughout development, therefore, there is a mismatch between the numbers of Kv alpha subunits expressed and the functional voltage-gated K+ channel currents distinguished electrophysiologically in rat ventricular myocytes. Alternative experimental approaches will be required to define directly the Kv alpha subunits that underlie functional voltage-gated K+ channels in these (and other) cells. In addition, the finding that Kv alpha subunit protein expression levels do not necessarily mirror mRNA levels suggests that caution should be exercised in attempting functional interpretations of observed changes in mRNA levels alone.

SKF-96365 strongly inhibits voltage-gated sodium current in rat ventricular myocytes

2014 ◽  
Vol 467 (6) ◽  
pp. 1227-1236 ◽  
Author(s):  
Kui-Hao Chen ◽  
Hui Liu ◽  
Lei Yang ◽  
Man-Wen Jin ◽  
Gui-Rong Li
Keyword(s):  
Ventricular Myocytes ◽  
Sodium Current ◽  
Voltage Gated ◽  
Rat Ventricular Myocytes

scholarly journals Effects of thrombospondin-4 on voltage-gated ion channels in rat ventricular myocytes

2020 ◽  
Vol 93 (0) ◽  
pp. 3-P-329
Author(s):  
Muneyoshi Okada ◽  
Momoko Aratani ◽  
Takahiro Koyama ◽  
Keisuke Imoto ◽  
Hideyuki Yamawaki
Keyword(s):  
Ion Channels ◽  
Ventricular Myocytes ◽  
Voltage Gated ◽  
Rat Ventricular Myocytes ◽  
Voltage Gated Ion Channels ◽  
Thrombospondin 4 ◽  
Gated Ion Channels

CARP Plays a Key Role in Cellular Growth Under Hypertrophic Stimuli in Neonatal Rat Ventricular Myocytes

2013 ◽  
Vol 9 ◽  
Author(s):  
Tara A Shrout
Keyword(s):  
Gene Expression ◽  
Ventricular Myocytes ◽  
Cellular Growth ◽  
Neonatal Rat ◽  
Transcriptional Level ◽  
Dual Nature ◽  
Hypertrophic Growth ◽  
Rat Ventricular Myocytes ◽  
Neonatal Rat Ventricular Myocytes ◽  
Over Time

Cardiac hypertrophy is a growth process that occurs in response to stress stimuli or injury, and leads to the induction of several pathways to alter gene expression. Under hypertrophic stimuli, sarcomeric structure is disrupted, both as a consequence of gene expression and local changes in sarcomeric proteins. Cardiac-restricted ankyrin repeat protein (CARP) is one such protein that function both in cardiac sarcomeres and at the transcriptional level. We postulate that due to this dual nature, CARP plays a key role in maintaining the cardiac sarcomere. GATA4 is another protein detected in cardiomyocytes as important in hypertrophy, as it is activated by hypertrophic stimuli, and directly binds to DNA to alter gene expression. Results of GATA4 activation over time were inconclusive; however, the role of CARP in mediating hypertrophic growth in cardiomyocytes was clearly demonstrated. In this study, Neonatal Rat Ventricular Myocytes were used as a model to detect changes over time in CARP and GATA4 under hypertrophic stimulation by phenylephrine and high serum media. Results were detected by analysis of immunoblotting. The specific role that CARP plays in mediating cellular growth under hypertrophic stimuli was studied through immunofluorescence, which demonstrated that cardiomyocyte growth with hypertrophic stimulation was significantly blunted when NRVMs were co-treated with CARP siRNA. These data suggest that CARP plays an important role in the hypertrophic response in cardiomyocytes.

Estimation of myofibrillar responsiveness to Ca 2+ in isolated rat ventricular myocytes

1998 ◽  
Vol 436 (5) ◽  
pp. 639-645 ◽  
Author(s):  
K. Hongo ◽  
Yoichiro Kusakari ◽  
Masato Konishi ◽  
Satoshi Kurihara ◽  
Seibu Mochizuki
Keyword(s):  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes ◽  
Isolated Rat

Dofetilide Enhances the Contractility of Rat Ventricular Myocytes via Augmentation of Na+–Ca2+ Exchange

2009 ◽  
Vol 23 (3) ◽  
pp. 207-214 ◽  
Author(s):  
Xuan-Ping Zhang ◽  
Bo-Wei Wu ◽  
Cai-Hong Yang ◽  
Jie Wang ◽  
Shuan-Cheng Niu ◽  
...  
Keyword(s):  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes
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