Compartmentalization of RNA Processing Factors within Nuclear Speckles

2000 ◽  
Vol 129 (2-3) ◽  
pp. 241-251 ◽  
Author(s):  
Paul J. Mintz ◽  
David L. Spector
Keyword(s):  
Rna Processing ◽  
Nuclear Speckles ◽  
Processing Factors

scholarly journals Coordination of transcription, processing, and export of highly expressed RNAs by distinct biomolecular condensates

2020 ◽  
Vol 4 (3) ◽  
pp. 281-291 ◽  
Author(s):  
Alexander M. Ishov ◽  
Aishwarya Gurumurthy ◽  
Jörg Bungert
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rna Processing ◽  
Transcription Initiation ◽  
Nuclear Speckles ◽  
High Concentration ◽  
Pol Ii ◽  
Processing Factors

Genes under control of super-enhancers are expressed at extremely high levels and are frequently associated with nuclear speckles. Recent data suggest that the high concentration of unphosphorylated RNA polymerase II (Pol II) and Mediator recruited to super-enhancers create phase-separated condensates. Transcription initiates within or at the surface of these phase-separated droplets and the phosphorylation of Pol II, associated with transcription initiation and elongation, dissociates Pol II from these domains leading to engagement with nuclear speckles, which are enriched with RNA processing factors. The transitioning of Pol II from transcription initiation domains to RNA processing domains effectively co-ordinates transcription and processing of highly expressed RNAs which are then rapidly exported into the cytoplasm.

scholarly journals In Arabidopsis thaliana mitochondria 5′ end polymorphisms of nad4L-atp4 and nad3-rps12 transcripts are linked to RNA PROCESSING FACTORs 1 and 8

2021 ◽  
Author(s):  
Sarah Schleicher ◽  
Stefan Binder
Keyword(s):  
Arabidopsis Thaliana ◽  
Rna Processing ◽  
Degradation Products ◽  
Underlying Mechanism ◽  
Transcriptional Level ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
P Type ◽  
Pentatricopeptide Repeat Proteins ◽  
Processing Factors

Abstract Key message RNA PROCESSING FACTORs 1 AND 8 (RPF1 and RPF8), both restorer of fertility like pentatricopeptide repeat proteins, are required for processing of dicistronic nad4L-atp4 and nad3-rps12 transcripts in Arabidopsis mitochondria. Abstract In mitochondria of Arabidopsis thaliana (Arabidopsis), the 5′ termini of many RNAs are generated on the post-transcriptional level. This process is still poorly understood in terms of both the underlying mechanism as well as proteins required. Our studies now link the generation of polymorphic 5′ extremities of the dicistronic nad3-rps12 and nad4L-atp4 transcripts to the function of the P-type pentatricopeptide repeat proteins RNA PROCESSING FACTORs 8 (RPF8) and 1 (RPF1). RPF8 is required to generate the nad3-rps12 -141 5′ end in ecotype Van-0 whereas the RPF8 allele in Col has no function in the generation of any 5′ terminus of this transcript. This observation strongly suggests the involvement of an additional factor in the generation of the -229 5′ end of nad3-rps12 transcripts in Col. RPF1, previously found to be necessary for the generation of the -228 5′ end of the major 1538 nucleotide-long nad4 mRNAs, is also important for the formation of nad4L-atp4 transcripts with a 5′ end at position -318 in Col. Many Arabidopsis ecotypes contain inactive RPF1 alleles resulting in the accumulation of various low abundant nad4L-atp4 RNAs which might represent precursor and/or degradation products. Some of these ecotypes accumulate major, but slightly smaller RNA species. The introduction of RPF1 into these lines not only establishes the formation of the major nad4L-atp4 dicistronic mRNA with the -318 5′ terminus, the presence of this gene also suppresses the accumulation of most alternative nad4L-atp4 RNAs. Beside RPF1, several other factors contribute to nad4L-atp4 transcript formation.

scholarly journals Pre-Messenger RNA Processing Factors in the Drosophila Genome

2000 ◽  
Vol 150 (2) ◽  
pp. F37-F44 ◽  
Author(s):  
Stephen M. Mount ◽  
Helen K. Salz
Keyword(s):  
Rna Processing ◽  
Messenger Rna ◽  
Drosophila Genome ◽  
Processing Factors

scholarly journals Emerging roles of RNA processing factors in regulating long non-coding RNAs

RNA Biology ◽  
2014 ◽  
Vol 11 (7) ◽  
pp. 793-797 ◽  
Author(s):  
Huiming Zhang ◽  
Jian-Kang Zhu
Keyword(s):  
Rna Processing ◽  
Non Coding Rnas ◽  
Processing Factors

scholarly journals A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors

2011 ◽  
Vol 30 (10) ◽  
pp. 1953-1964 ◽  
Author(s):  
Cristina Tous ◽  
Ana G Rondón ◽  
María García-Rubio ◽  
Cristina González-Aguilera ◽  
Rosa Luna ◽  
...  
Keyword(s):  
Rna Processing ◽  
Transcript Elongation ◽  
Processing Factors
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