Lack of Induction of Epithelial Cell Proliferation by Sodium Saccharin and Sodium L-Ascorbate in the Urinary Bladder of NCI-Black-Reiter (NBR) Male Rats

1994 ◽  
Vol 127 (2) ◽  
pp. 182-186 ◽  
Author(s):  
S. Uwagawa ◽  
K. Saito ◽  
Y. Okuno ◽  
H. Kawasaki ◽  
A. Yoshitake ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Urinary Bladder ◽  
Male Rats ◽  
Epithelial Cell Proliferation ◽  
Sodium Saccharin

scholarly journals Enhanced intestinal epithelial cell proliferation in diabetic rats correlates with β-catenin accumulation

2015 ◽  
Vol 226 (3) ◽  
pp. 135-143 ◽  
Author(s):  
Tatiana Dorfman ◽  
Yulia Pollak ◽  
Rima Sohotnik ◽  
Arnold G Coran ◽  
Jacob Bejar ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Intestinal Epithelial Cell ◽  
Diabetic Rats ◽  
Male Rats ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation

The Wnt/β-catenin signaling cascade is implicated in the control of stem cell activity, cell proliferation, and cell survival of the gastrointestinal epithelium. Recent evidence indicates that the Wnt/β-catenin pathway is activated under diabetic conditions. The purpose of this study was to evaluate the role of Wnt/β-catenin signaling during diabetes-induced enteropathy in a rat model. Male rats were divided into three groups: control rats received injections of vehicle; diabetic rats received injections of one dose of streptozotocin (STZ); and diabetic–insulin rats received injections of STZ and were treated with insulin given subcutaneously at a dose of 1 U/kg twice daily. Rats were killed on day 7. Wnt/β-catenin-related genes and expression of proteins was determined using real-time PCR, western blotting, and immunohistochemistry. Among 13 genes identified by real-time PCR, seven genes were upregulated in diabetic rats compared with control animals including the target genes c-Myc and Tcf4. Diabetic rats also showed a significant increase in β-catenin protein compared with control animals. Treatment of diabetic rats attenuated the stimulating effect of diabetes on intestinal cell proliferation and Wnt/β-catenin signaling. In conclusion, enhanced intestinal epithelial cell proliferation in diabetic rats correlates with β-catenin accumulation.

scholarly journals Significant induction of epithelial cell proliferation in the urinary bladders of NCI-black-reiter male rats by N-butyl-N-(4-hydroxybutyl) nitrosamine.

1995 ◽  
Vol 8 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Katsumi Takaba ◽  
Yoshihiro Nakagawa ◽  
Seiichi Ito ◽  
Akira Yoshitake ◽  
Hideki Wanibuchi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Male Rats ◽  
Epithelial Cell Proliferation

scholarly journals Lack of Enhanced Epithelial Cell Proliferation in the Urinary Bladder of Heterozygous p53 Knockout Mice Given Sodium Ortho-phenylphenate or Uracil

2003 ◽  
Vol 16 (3) ◽  
pp. 147-152
Author(s):  
Satoshi Uwagawa ◽  
Keisuke Ozaki ◽  
Tokuo Sukata ◽  
Masahiko Kushida ◽  
Yasuyoshi Okuno ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Urinary Bladder ◽  
Knockout Mice ◽  
Epithelial Cell Proliferation

scholarly journals Deaf-1 regulates epithelial cell proliferation and side-branching in the mammary gland

2008 ◽  
Vol 8 (1) ◽  
pp. 94 ◽  
Author(s):  
Holly E Barker ◽  
Gordon K Smyth ◽  
James Wettenhall ◽  
Teresa A Ward ◽  
Mary L Bath ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mammary Gland ◽  
Epithelial Cell ◽  
Epithelial Cell Proliferation

scholarly journals c-Src inactivation reduces renal epithelial cell-matrix adhesion, proliferation, and cyst formation

2011 ◽  
Vol 301 (2) ◽  
pp. C522-C529 ◽  
Author(s):  
Justine Elliott ◽  
Nadezhda N. Zheleznova ◽  
Patricia D. Wilson
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Collecting Duct ◽  
Specific Inhibitor ◽  
Cell Phenotype ◽  
Epithelial Cell Proliferation ◽  
Matrix Adhesion ◽  
Cyst Lining

c-Src is a non-receptor tyrosine kinase whose activity is induced by phosphorylation at Y418 and translocation from the cytoplasm to the cell membrane. Increased activity of c-Src has been associated with cell proliferation, matrix adhesion, motility, and apoptosis in tumors. Immunohistochemistry suggested that activated (pY418)-Src activity is increased in cyst-lining autosomal dominant polycystic kidney disease (ADPKD) epithelial cells in human and mouse ADPKD. Western blot analysis showed that SKI-606 (Wyeth) is a specific inhibitor of pY418-Src without demonstrable effects on epidermal growth factor receptor or ErbB2 activity in renal epithelia. In vitro studies on mouse inner medullary collecting duct (mIMCD) cells and human ADPKD cyst-lining epithelial cells showed that SKI-606 inhibited epithelial cell proliferation over a 24-h time frame. In addition, SKI-606 treatment caused a striking statistically significant decrease in adhesion of mIMCD and human ADPKD to extracellular collagen matrix. Retained viability of unattached cells was consistent with a primary effect on epithelial cell anchorage dependence mediated by the loss of extracellular matrix (ECM)-attachment due to α2β1-integrin function. SKI-606-mediated attenuation of the human ADPKD hyperproliferative and hyper-ECM-adhesive epithelial cell phenotype in vitro was paralleled by retardation of the renal cystic phenotype of Pkd1 orthologous ADPKD heterozygous mice in vivo. This suggests that SKI-606 has dual effects on cystic epithelial cell proliferation and ECM adhesion and may have therapeutic potential for ADPKD patients.

scholarly journals Isoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro

1999 ◽  
Vol 80 (10) ◽  
pp. 1550-1557 ◽  
Author(s):  
C Booth ◽  
D F Hargreaves ◽  
J A Hadfield ◽  
A T McGown ◽  
C S Potten
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation
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