Pilot-scale Treatment of TNT-spiked Groundwater by Hybrid Poplar Trees

Author(s):  
Phillip L. Thompson ◽  
Liz A. Ramer ◽  
Pu Yong ◽  
Jerald L. Schnoor
2001 ◽  
Vol 3 (1) ◽  
pp. 87-104 ◽  
Author(s):  
A. Ferro ◽  
J. Chard ◽  
R. Kjelgren ◽  
B. Chard ◽  
D. Turner ◽  
...  
Keyword(s):  

2007 ◽  
Vol 85 (11) ◽  
pp. 1071-1081 ◽  
Author(s):  
Edward J. Harrison ◽  
Michael Bush ◽  
Jonathan M. Plett ◽  
Daniel P. McPhee ◽  
Robin Vitez ◽  
...  

We have produced the largest population of activation-tagged poplar trees to date, approximately 1800 independent lines, and report on phenotypes of interest that have been identified in tissue culture and greenhouse conditions. Activation tagging is an insertional mutagenesis technique that results in the dominant upregulation of an endogenous gene. A large-scale Agrobacterium -mediated transformation protocol was used to transform the pSKI074 activation-tagging vector into Populus tremula × Populus alba hybrid poplar. We have screened the first 1000 lines for developmental abnormalities and have a visible mutant frequency of 2.4%, with alterations in leaf and stem structure as well as overall stature. Most of the phenotypes represent new phenotypes that have not previously been identified in poplar and, in some cases, not in any other plant either. Molecular analysis of the T-DNA inserts of a subpopulation of mutant lines reveal both single and double T-DNA inserts with double inserts more common in lines with visible phenotypes. The broad range of developmental mutants identified in this pilot screen of the population reveals that it will be a valuable resource for gene discovery in poplar. The full value of this population will only be realized as we screen these lines for a wide range of phenotypes.


2004 ◽  
Vol 6 (2) ◽  
pp. 157-167 ◽  
Author(s):  
Xingmao Ma ◽  
Andrew R. Richter ◽  
Sarah Albers ◽  
Joel G. Burken
Keyword(s):  

2021 ◽  
Author(s):  
Minglei Yi ◽  
Heyu Yang ◽  
Shaohui Yang ◽  
Jiehua Wang

Plant branching is usually prevented by an actively proliferating apex. In poplars, one GRAS family member, SHORT-ROOT2 (PtSHR2), was preferentially expressed in axillary buds (AXBs) and was inducible during bud maturation and activation. Overexpression of PtSHR2 (PtSHR2OE) in hybrid poplar impaired the apical dominance and simultaneously promoted the outgrowth of axillary branches below the maturation point (BMP), accompanied by regulated expression of genes critical for axillary meristem initiation and bud formation. Following a detained phenotypic characterization, we compared the IAA and trans-zeatin levels in apical shoots and AXBs of wild-type and PtSHR2OE trees, together with gene expression analyses and defoliation, decapitation, and hormone reapplication assays. PtSHR2OE AXBs contained a significantly lower ratio of auxin to cytokinin than wild-type AXBs, particularly in those below the BMP. Decapitation induced a faster bud burst in PtSHR2OE trees than in wild-type plants, and it could be strongly inhibited by exogenously applied auxin and cytokinin biosynthesis inhibitor, but only partially inhibited by N-1-naphthylphthalamic acid (NPA). An impaired basipetal auxin transport, rather than an insufficient auxin biosynthesis or auxin insensitivity, disturbed the local hormonal homeostasis in PtSHR2OE AXBs, which in turn enhanced the axillary bud initiation and promoted the bud release.


Author(s):  
Sachin Naik ◽  
Sylvester Palys ◽  
Marcos Di Falco ◽  
Adrian Tsang ◽  
Pierre Périnet ◽  
...  

Species of the genus Populus commonly known as poplars are one of the most widely used groups of forest trees in North America and Europe, and play a significant ecological role as a pioneer species in boreal forests, and as a dominant species in the riparian forests that serve as wildlife habitats and watersheds. Natural and artificial hybrids of poplars are being extensively used in commercial plantations. However, many hybrid poplar trees are susceptible to Sphaerulina musiva, the pathogenic fungus that causes leaf spots and stem cankers and limits the utility of hybrid poplars as a plantation trees. We isolated an endophytic bacterial strain Bacillus velezensis EB14 from a Populus hybrid which showed a strong antifungal activity against S. musiva. Through mass spectrometric analyses of co-cultured B. velezensis EB14 and S. musiva, we identified five cyclic lipopeptides produced by B. velezensis EB14 – Iturin A1, Iturin A2, Iturin A9, Subtulene A and Fengycin. In addition, B. velezensis EB14 produced four major unidentified compounds in co-cultivation with S. musiva. The cyclopeptide production by B. velezensis EB14 was more pronounced (20-1000 fold) in the co-cultured plates due to elicitation by S. musiva. We also discovered that the native endophytic B. velezensis EB14 strain exhibited different levels of interactions against the endophytic fungal microbiomes of Populus sp. Overall, our results indicate B. velezensis EB14 strain as a promising biocontrol agent that could be used against stem canker and leaf spot diseases caused by S. musiva in Poplar plantations.


1972 ◽  
Vol 22 (5) ◽  
pp. 369-371 ◽  
Author(s):  
Leon S. Dochinger ◽  
Alden M. Townsend ◽  
Donald W. Seegrist ◽  
Frederick W. Bender

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