Cyclic Sequence Comparison Using Dynamic Warping

Abstract Background Although some studies have investigated the bacterial community in vaginal tract of pregnant women, there are few reports about the viral community (virome) in this type of microenvironment. Methods To investigate the composition of virome in vaginal secretion samples, 40 vaginal secretion samples from pregnant women with vaginitis and 20 vaginal secretion samples from pregnant women without vaginitis, pooled into 4 and 2 sample pools, respectively, were subjected to viral metagenomic analysis. Results Results indicated virus sequences showing similarity to human papillomavirus (HPV), anellovirus, and norovirus were recovered from this cohort of pregnant women. Further analysis indicated that 15 different defined types and one unclassified type of HPV were detected from pregnant women with vaginitis while only 3 defined types of HPV were detected in pregnant women without vaginitis. Five different groups of viruses from the family Anelloviridae were present in pregnant women with but none of them were detected in pregnant women without vaginitis. Norovirus was detected in 3 out of the 4 sample pools from pregnant women with vaginitis but none in the pregnant women without vaginitis. Twelve complete genomes belonging to 10 different types of HPV, and 5 novel anllovirus genomes belonging 2 different genera in Anelloviridae were acquired from these libraries, based on which phylogenetical analysis and pairwise sequence comparison were performed. Phageome in these samples was also briefly characterized and compared between two groups. Conclusion Our data suggested that virome might play an important role in the progression of vaginitis in pregnant women.

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Mouse "protective protein". cDNA cloning, sequence comparison, and expression.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)39616-4 ◽

1990 ◽

Vol 265 (8) ◽

pp. 4678-4684

Author(s):

N J Galjart ◽

N Gillemans ◽

D Meijer ◽

A d'Azzo

Keyword(s):

Cdna Cloning ◽

Sequence Comparison

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The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus)

Pathogens ◽

10.3390/pathogens10070853 ◽

2021 ◽

Vol 10 (7) ◽

pp. 853

Author(s):

Jacek Karamon ◽

Małgorzata Samorek-Pieróg ◽

Jacek Sroka ◽

Ewa Bilska-Zając ◽

Joanna Dąbrowska ◽

...

Keyword(s):

Small Intestine ◽

Molecular Identification ◽

Canis Lupus ◽

Sequence Comparison ◽

First Record ◽

Grey Wolf ◽

South Eastern ◽

Counting Technique ◽

Small Intestines ◽

First Time

The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected Echinococcus tapeworms were isolated and identified by PCRs and sequencing (nad1 and cox1 genes). Additionally, DNA isolated from the feces of wolves positive for Echinococcus tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for E. granulosus s.l. when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detected 61 adult tapeworms: 42 in the anterior, 14 in the middle, and 5 in the posterior parts of the small intestine. The PCRs conducted for cox1 and nad1 produced specific products. A sequence comparison with the GenBank database showed similarity to the deposited E. ortleppi (G5) sequences. An analysis of the available phylogenetic sequences showed very little variation within the species of E. ortleppi (G5), and identity ranged from 99.10% to 100.00% in the case of cox1 and from 99.04 to 100.00% in the case of nad1. One of the two diagnostic PCRs used and performed on the feces of Echinococcus-positive animals showed product specific for E. granulosus. This study showed the presence of adult E. ortleppi tapeworms in wolves for the first time.

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Cloning and sequence comparison ofAvaI andBsoBI restriction-modification systems

MGG Molecular & General Genetics ◽

10.1007/bf02173975 ◽

1996 ◽

Vol 252 (6) ◽

pp. 695-699 ◽

Cited By ~ 3

Author(s):

H. Ruan ◽

K. D. Lunnen ◽

M. E. Scott ◽

L. S. Moran ◽

B. E. Slatko ◽

...

Keyword(s):

Sequence Comparison ◽

Restriction Modification ◽

Restriction Modification Systems

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