Electrospun Antimicrobial Wound Dressings: Novel Strategies to Fight Against Wound Infections

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

Pharmaceutics ◽

10.3390/pharmaceutics12090840 ◽

2020 ◽

Vol 12 (9) ◽

pp. 840 ◽

Cited By ~ 3

Author(s):

Viorica Patrulea ◽

Gerrit Borchard ◽

Olivier Jordan

Keyword(s):

Antimicrobial Peptides ◽

Bacterial Infections ◽

Antimicrobial Agents ◽

New Technologies ◽

Multidrug Resistant ◽

Inorganic Nanoparticles ◽

Wound Dressings ◽

Wound Infections ◽

Clinical Phase ◽

Delivery Strategies

Bacterial infections occur when wound healing fails to reach the final stage of healing, which is usually hindered by the presence of different pathogens. Different topical antimicrobial agents are used to inhibit bacterial growth due to antibiotic failure in reaching the infected site, which is accompanied very often by increased drug resistance and other side effects. In this review, we focus on antimicrobial peptides (AMPs), especially those with a high potential of efficacy against multidrug-resistant and biofilm-forming bacteria and fungi present in wound infections. Currently, different AMPs undergo preclinical and clinical phase to combat infection-related diseases. AMP dendrimers (AMPDs) have been mentioned as potent microbial agents. Various AMP delivery strategies that are used to combat infection and modulate the healing rate—such as polymers, scaffolds, films and wound dressings, and organic and inorganic nanoparticles—have been discussed as well. New technologies such as Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated protein (CRISPR-Cas) are taken into consideration as potential future tools for AMP delivery in skin therapy.

Download Full-text

Antibiotic-Loaded Psyllium Husk Hemicellulose and Gelatin-Based Polymeric Films for Wound Dressing Application

Pharmaceutics ◽

10.3390/pharmaceutics13020236 ◽

2021 ◽

Vol 13 (2) ◽

pp. 236

Author(s):

Naveed Ahmad ◽

Muhammad Masood Ahmad ◽

Nabil K. Alruwaili ◽

Ziyad Awadh Alrowaili ◽

Fadhel Ahmed Alomar ◽

...

Keyword(s):

Antibacterial Activity ◽

Drug Release ◽

Wound Dressing ◽

Chronic Wounds ◽

Wound Dressings ◽

Patient Comfort ◽

Wound Infections ◽

Psyllium Husk ◽

Antibiotic Release

Wound infections are one of the major reasons for the delay in the healing of chronic wounds and can be overcome by developing effective wound dressings capable of absorbing exudate, providing local antibiotic release, and improving patient comfort. Arabinoxylan (AX) is a major hemicellulose present in psyllium seed husk (PSH) and exhibits promising characteristics for developing film dressings. Herein, AX-gelatin (GL) films were prepared by blending AX, gelatin (GL), glycerol, and gentamicin (antibiotic). Initially, the optimal quantities of AX, GL, and glycerol for preparing transparent, bubble-free, smooth, and foldable AX-GL films were found. Physiochemical, thermal, morphological, drug release, and antibacterial characteristics of the AX-GL films were evaluated to investigate their suitability as wound dressings. The findings suggested that the mechanical, water vapor transmission, morphological, and expansion characteristics of the optimized AX-GL films were within the required range for wound dressing. The results of Fourier-transform infrared (FTIR) analyses suggested chemical compatibility among the ingredients of the films. In in vitro drug release and antibacterial activity experiments, gentamicin (GM)-loaded AX-GL films released approximately 89% of the GM in 24 h and exhibited better antibacterial activity than standard GM solution. These results suggest that AX-GL films could serve as a promising dressing to protect against wound infections.

Download Full-text

Effects of Topical Antimicrobial Formulations on Pseudomonas aeruginosa Biofilm in an In vivo Porcine Burn Wound Model

10.1101/737338 ◽

2019 ◽

Author(s):

S.C. Davis ◽

M. Solis ◽

J. Gil ◽

J. Valdes ◽

A. Higa ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Wound Healing ◽

Post Infection ◽

Wound Dressings ◽

Wound Infections ◽

Burn Wound ◽

Bacterial Counts ◽

Burn Wounds ◽

Wound Model

AbstractSilver has been incorporated into a variety of wound dressings and topical agents to prevent and combat wound infections. Pseudomonas aeruginosa is a common cause of burn wound infections and well-known biofilm producer. The objective of this study was to evaluate the effects of a panel of wound dressings containing different silver formulations on P. aeruginosa biofilms using an in vivo porcine burn wound model. Second-degree burns were created on the skin of specific pathogen-free pigs (n = 3) and inoculated with 2.14 × 105 cfu P. aeruginosa per wound. Biofilms were allowed to develop for 24 h, and then each wound was treated with one of 6 treatments: silver oxynitrate dressing (OXY), silver oxynitrate powder (POWD), nanocrystalline silver dressing (NANO), silver chloride dressing (AGCL), silver sulfadiazine (SSD), or a negative control polyurethane film with no silver-based formulation (NEG). Wounds were cultured at D3 post-infection (n = 3 per pig per treatment) and at D6 post-infection (n = 3 per pig per treatment) for quantification of bacteria. On D6, biopsies (n = 3 per treatment) were taken from POWD, SSD, and NEG wounds and wound healing progress was evaluated histologically. At the time of treatment initiation, 24 h post-infection, 8.71 log cfu P. aeruginosa were present in burn wounds. On D3 and D6, all treatments significantly reduced bacterial counts in wounds as compared to NEG, but POWD caused an approximately 7-log reduction in bacterial counts on both days and was the only treatment to reduce the bacterial counts to below the threshold for detecting bacteria. The OXY, NANO, and SSD treatments had similar reductions in bacterial recovery on D3 and D6 of approximately 2.5-4 log. The histological healing metrics of reepithelialization percentage, epithelial thickness, white cell infiltration, angiogenesis, and granulation tissue formation were similar among wounds from POWD, SSD, and NEG groups at 6 days post-infection. Silver oxynitrate powder reduced P. aeruginosa growth in burn wounds more effectively than other silver-based dressings but did not impact wound healing.

Download Full-text

The Insight of Body’s Immune System, Inflammation and Damages in Wound Healing- The review

International Journal for Research in Applied Science and Engineering Technology ◽

10.22214/ijraset.2021.37868 ◽

2021 ◽

Vol 9 (8) ◽

pp. 2777-2780

Author(s):

Priyanka Patil

Keyword(s):

Wound Healing ◽

Immune System ◽

Wound Dressing ◽

Wound Dressings ◽

Wound Infections ◽

Wound Area ◽

Wound Site ◽

Healing Wound ◽

Dressing Materials ◽

Keywords Wound Healing

Abstract: The injuries and its infections are the most painful form of trauma. Wound infections are the growth of microorganisms within the wound area. This infection causes the body’s immune system, inflammation and damages the tissue within the wound site. Hence, there is an immense need to formulate new dressing materials for wound dressing application. To know more about new dressing material working it is need of time to study the mechanism of wound healing. Today’s review focus on mechanism of wound healing, wound dressing, new dressing material and necessity of wound dressings. Keywords: Wound healing, dressing material

Download Full-text

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

10.20944/preprints202007.0375.v1 ◽

2020 ◽

Author(s):

Viorica Patrulea ◽

Gerrit Borchard ◽

Olivier Jordan

Keyword(s):

Antimicrobial Peptides ◽

Bacterial Infections ◽

Antimicrobial Agents ◽

New Technologies ◽

Multidrug Resistant ◽

Inorganic Nanoparticles ◽

Wound Dressings ◽

Wound Infections ◽

Clinical Phase ◽

Delivery Strategies

Bacterial infections occur when wound healing fails to reach the final stage of healing, usually hindered by the presence of different pathogens. Different topical antimicrobial agents are used to inhibit bacterial growth due to antibiotic failure in reaching the infected site accompanied very often by an increased drug resistance and other side effects. In this review, we focus on antimicrobial peptides (AMPs), especially those with a high potential of efficacy against multidrug-resistant and biofilm-forming bacteria and fungi present in wound infections. Currently, different AMPs undergo preclinical and clinical phase to combat infection-related diseases. AMP dendrimers (AMPDs) have been mentioned as potent microbial agents. Various AMP delivery strategies, such as polymers, scaffolds, films and wound dressings, organic and inorganic nanoparticles, to combat infection and modulate the healing rate have been discussed as well. New technologies such as CRISPR-Cas are taken into consideration as potential future tools for AMP delivery in skin therapy.

Download Full-text

Healing of Chronic Wounds by Copper Oxide-Impregnated Wound Dressings—Case Series

Medicina ◽

10.3390/medicina57030296 ◽

2021 ◽

Vol 57 (3) ◽

pp. 296

Author(s):

Eyal Melamed ◽

Patrick Kiambi ◽

Dancan Okoth ◽

Irena Honigber ◽

Eran Tamir ◽

...

Keyword(s):

Wound Healing ◽

Copper Oxide ◽

Chronic Wounds ◽

Case Reports ◽

Case Series ◽

Skin Regeneration ◽

Wound Dressings ◽

Treatment Modalities ◽

Wound Infections ◽

Infected Wounds

Novel antimicrobial wound dressings impregnated with copper oxide micro-particles have been cleared for treatment of acute and chronic wounds. Our objective is to provide preliminary data regarding the potential benefit of using these novel wound dressings including in non-infected wounds. Methods involved the treatment of wounds that responded partially or poorly to conventional wound healing treatments with copper oxide impregnated wound dressings in patients with a range of etiologies. Ten cases of patients with etiologies such as diabetes mellitus, sickle cell disease, renal failure, and necrotizing fasciitis, in which the application of copper oxide impregnated wound dressings in infected and non-infected wounds, which resulted in significant enhanced wound healing, are presented. This was exemplified by clearing of the wound infections, reduction of the fibrous and/or necrotic tissue and by intense granulation, epithelialization, and wound closure. The described 10 case reports support our hypothesis that the copper oxide-containing wound dressing not only confers protection to the wound and the dressing from microbial contamination, and in some cases may help clear the wound infections, but in addition and more importantly, stimulate skin regeneration and wound healing. Our findings are in line with previous animal and in vitro studies showing that copper plays a key role in angiogenesis and skin regeneration. These case reports support the notion that the use of copper oxide impregnated wound dressings may be an important intervention in the arsenal of wound treatment modalities, especially in hard to heal wounds.

Download Full-text