Dual Polarisation Interferometry: An Optical Technique to Measure the Orientation and Structure of Proteins at the Solid-Liquid Interface in Real Time

2006 ◽  
pp. 75-104 ◽  
Author(s):  
Neville Freeman
Keyword(s):  
Real Time ◽  
Liquid Interface ◽  
Optical Technique ◽  
Dual Polarisation Interferometry ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Structure Of Proteins

Quantification of the effects of melittin on liposome structure

2005 ◽  
Vol 33 (5) ◽  
pp. 931-933 ◽  
Author(s):  
J. Popplewell ◽  
N. Freeman ◽  
S. Carrington ◽  
G. Ronan ◽  
C. McDonnell ◽  
...  
Keyword(s):  
Real Time ◽  
Rupture Process ◽  
Liquid Interface ◽  
Optical Technique ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Lipid Structures

An optical technique, dual-polarization interferometry, has been used to examine lipid structures at the solid/liquid interface. Changes in the lipid structures, in real time, were examined as a consequence of challenging them with a peptide (melittin) that is known to induce liposome rupture. This work suggests that it should be possible to obtain a better understanding of the detail of the melittin rupture process.

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