Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation Phenotyping of Stem Cells

2013 ◽  
pp. 77-88 ◽  
Author(s):  
Jian Tajbakhsh
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Protein Biomarkers

scholarly journals Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Peng-Fei Xu ◽  
Ricardo Moraes Borges ◽  
Jonathan Fillatre ◽  
Maraysa de Oliveira-Melo ◽  
Tao Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Tracking ◽  
Cardiac Tissue ◽  
Embryonic Stem ◽  
Mammalian Embryo ◽  
Wide Range ◽  
Vitro Model ◽  
Neurula Stage

AbstractGenerating properly differentiated embryonic structures in vitro from pluripotent stem cells remains a challenge. Here we show that instruction of aggregates of mouse embryonic stem cells with an experimentally engineered morphogen signalling centre, that functions as an organizer, results in the development of embryo-like entities (embryoids). In situ hybridization, immunolabelling, cell tracking and transcriptomic analyses show that these embryoids form the three germ layers through a gastrulation process and that they exhibit a wide range of developmental structures, highly similar to neurula-stage mouse embryos. Embryoids are organized around an axial chordamesoderm, with a dorsal neural plate that displays histological properties similar to the murine embryo neuroepithelium and that folds into a neural tube patterned antero-posteriorly from the posterior midbrain to the tip of the tail. Lateral to the chordamesoderm, embryoids display somitic and intermediate mesoderm, with beating cardiac tissue anteriorly and formation of a vasculature network. Ventrally, embryoids differentiate a primitive gut tube, which is patterned both antero-posteriorly and dorso-ventrally. Altogether, embryoids provide an in vitro model of mammalian embryo that displays extensive development of germ layer derivatives and that promises to be a powerful tool for in vitro studies and disease modelling.

The osteogenic differentiation of rat muscle-derived stem cells in vivo within in situ-forming chitosan scaffolds

Biomaterials ◽  
2008 ◽  
Vol 29 (33) ◽  
pp. 4420-4428 ◽  
Author(s):  
Kyung Sook Kim ◽  
Jung Hwa Lee ◽  
Hyun Hee Ahn ◽  
Ju Young Lee ◽  
Gilson Khang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Rat Muscle ◽  
In Situ Forming ◽  
Chitosan Scaffolds

scholarly journals Genome-Wide DNA Methylation Pattern of Cancer Stem Cells in Esophageal Cancer

2020 ◽  
Vol 19 ◽  
pp. 153303382098379
Author(s):  
Xiying Yu ◽  
Ying Teng ◽  
Xingran Jiang ◽  
Hui Yuan ◽  
Wei Jiang
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Esophageal Cancer ◽  
Cancer Stem Cells ◽  
Side Population ◽  
Methylation Status ◽  
Methylation Profile ◽  
Cpg Dinucleotides ◽  
Genome Wide ◽  
Differentially Methylated Genes

Background: Cancer stem cells (CSCs) are considered the main cause of cancer recurrence and metastasis, and DNA methylation is involved in the maintenance of CSCs. However, the methylation profile of esophageal CSCs remains unknown. Methods: Side population (SP) cells were isolated from esophageal squamous cell carcinoma (ESCC) cell lines KYSE150 and EC109. Sphere-forming cells were collected from human primary esophageal cancer cells. SP cells and sphere-forming cells were used as substitutes for cancer stem-like cells. We investigated the genome-wide DNA methylation profile in esophageal cancer stem-like cells using reduced representation bisulfite sequencing (RRBS). Results: Methylated cytosine (mC) was found mostly in CpG dinucleotides, located mostly in the intronic, intergenic, and exonic regions. Forty intersected differentially methylated regions (DMRs) were identified in these 3 groups of samples. Thirteen differentially methylated genes with the same alteration trend were detected; these included OTX1, SPACA1, CD163L1, ST8SIA2, TECR, CADM3, GRM1, LRRK1, CHSY1, PROKR2, LINC00658, LOC100506688, and NKD2. DMRs covering ST8SIA2 and GRM1 were located in exons. These differentially methylated genes were involved in 10 categories of biological processes and 3 cell signaling pathways. Conclusions: When compared to non-CSCs, cancer stem-like cells have a differential methylation status, which provides an important biological base for understanding esophageal CSCs and developing therapeutic targets for esophageal cancer.

Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche

2008 ◽  
Vol 17 (7) ◽  
pp. 592-609 ◽  
Author(s):  
Jennifer Elisabeth Kloepper ◽  
Stephan Tiede ◽  
Jürgen Brinckmann ◽  
Dieter Peter Reinhardt ◽  
Wilfried Meyer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Bulge Region ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

scholarly journals Overexpression of CDKN2B (p15INK4B) and altered global DNA methylation status in mesenchymal stem cells of high-risk myelodysplastic syndromes

Leukemia ◽  
2014 ◽  
Vol 28 (11) ◽  
pp. 2241-2244 ◽  
Author(s):  
A Poloni ◽  
G Maurizi ◽  
D Mattiucci ◽  
S Amatori ◽  
B Fogliardi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Risk ◽  
Myelodysplastic Syndromes ◽  
Methylation Status ◽  
Global Dna Methylation

scholarly journals DNA methylation on N6-adenine in mammalian embryonic stem cells

Nature ◽  
2016 ◽  
Vol 532 (7599) ◽  
pp. 329-333 ◽  
Author(s):  
Tao P. Wu ◽  
Tao Wang ◽  
Matthew G. Seetin ◽  
Yongquan Lai ◽  
Shijia Zhu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem
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