Genetic Strategies on Kennedy Pathway to Improve Triacylglycerol Production in Oleaginous Rhodococcus Strains

2015 ◽  
pp. 121-139
Author(s):  
Martín A. Hernández ◽  
Héctor M. Alvarez
Keyword(s):  
Kennedy Pathway ◽  
Genetic Strategies

scholarly journals Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

2014 ◽  
Vol 41 (3) ◽  
pp. 321 ◽  
Author(s):  
Ronghua Xu ◽  
Tianquan Yang ◽  
Ruling Wang ◽  
Aizhong Liu
Keyword(s):  
Linoleic Acid ◽  
Jatropha Curcas ◽  
Molecular Mechanisms ◽  
Functional Divergence ◽  
Critical Role ◽  
Linoleic Acid Content ◽  
Transgenic Yeast ◽  
Kennedy Pathway ◽  
Tag Biosynthesis ◽  
Tag Accumulation

Diacylglycerol acyltransferases (DGATs) catalyse the final step of triacylglycerol (TAG) biosynthesis of the Kennedy pathway, and play a critical role during TAG accumulation in developing oleaginous seeds. In this study, the molecular cloning and characterisation of two DGAT genes, JcDGAT1 and JcDGAT2, from jatropha (Jatropha curcas L., a potential biodiesel plant) is presented. Using heterogonous overexpression techniques, both JcDGAT1 and JcDGAT2 were able to restore TAG biosynthesis in a yeast mutant H1246 strain, and enhance the quantity of TAG biosynthesis by 16.6 and 14.3%, respectively, in strain INVSc1. In transgenic tobacco, overexpression of JcDGAT1 and JcDGAT2 resulted in an increase in seed oil content of, respectively, 32.8 and 31.8%. Further, the functional divergence of JcDGAT1 and JcDGAT2 in TAG biosynthesis was demonstrated by comparing the fatty acid compositions in both the transgenic yeast and tobacco systems. In particular, JcDGAT2 incorporated a 2.5-fold higher linoleic acid content into TAG than JcDGAT1 in transgenic yeast and exhibited a significant linoleic acid substrate preference in both yeast and tobacco. This study provides new insights in understanding the molecular mechanisms of DGAT genes underlying the biosynthesis of linoleic acids and TAG in plants.

scholarly journals In VivoEvidence for the Specificity ofPlasmodium falciparumPhosphoethanolamine Methyltransferase and Its Coupling to the Kennedy Pathway

2005 ◽  
Vol 280 (13) ◽  
pp. 12461-12466 ◽  
Author(s):  
Gabriella Pessi ◽  
Jae-Yeon Choi ◽  
Jennifer M. Reynolds ◽  
Dennis R. Voelker ◽  
Choukri Ben Mamoun
Keyword(s):  
Kennedy Pathway

Recent Patents on Melanoma with Focus on Genetic Strategies

2008 ◽  
Vol 3 (2) ◽  
pp. 136-144
Author(s):  
Talia Gramiccia ◽  
Rosita Saraceno ◽  
Alessandro Stefani ◽  
Sergio Chimenti ◽  
Alessandro Stefani
Keyword(s):  
Genetic Strategies

15-Lipoxygenase-2 Deficiency Induces a Dysfunction in Macrophages That Can Be Restored by Salidroside

2021 ◽  
Author(s):  
Rong Huang Huang ◽  
Tingting Li Li ◽  
Xi Yong Yong ◽  
Huling Wen Wen ◽  
Xing Zhou Zhou ◽  
...  
Keyword(s):  
Natural Product ◽  
Signaling Pathway ◽  
Therapeutic Strategy ◽  
Caspase 3 ◽  
Mechanisms Of Action ◽  
Rna Seq ◽  
Immunological Function ◽  
Tnf Α ◽  
And Function ◽  
Genetic Strategies

Abstract 15-Lipoxygenase-2(15-LOX-2) is thought to regulate inflammation and immunological function however, its mechanisms of action are still unclear. Furthermore, it has been reported that salidroside has anti inflammatory properties , but its role in macrophage function has not been understood yet In this study, we aimed to determine how 15-LOX-2 expression level s affect the function of macrophages and the effect of salidroside on 15-LOX-2 deficient macrophages We used multiple functional genetic strategies to determine 15-LOX-2 function in macrophages. 15-LOX-2 deficiency promotes phagocytosis and proliferation of macrophages and impairs their apoptosis Mechanistically, t he expression levels of cyclophilinB (CypB) were upregulated in 15-LOX-2 deficient Ana 1 macrophages, whereas those of caspase 3 were down regulated. Furthermore, RNA-seq analysis showed that inflammation, complement, and TNF-α signaling pathway s were all activated in 15-LOX-2 deficient Ana 1 macrophages. Treatment of 15-LOX-2 deficient macrophages with salidroside, a natural product derived from Rhodiola species, effectively reversed the effects of 15-LOX-2 deficiency on caspase 3 and CypB levels, as well as on apoptosis and proliferation. In conclusion, our study shows that there is a newly identified link between 15-LOX-2 deficiency and salidroside in regulating macrophage survival, proliferation, and function. Salidroside may be a promising therapeutic strategy for treating inflammation related diseases resulting from 15-LOX-2 deficiency.

Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches

2019 ◽  
pp. 205-240
Author(s):  
Laura J. Smyth ◽  
Katie Kerr ◽  
Seamus Duffy ◽  
Jill Kilner ◽  
Amy Jayne McKnight
Keyword(s):  
Diabetic Nephropathy ◽  
Wet Lab ◽  
Genetic Strategies

Panel Discussion: Comparison of Traditional and Genetic Strategies

1984 ◽  
pp. 267-273
Author(s):  
Ross D. Brown ◽  
Joel S. Hirschhorn ◽  
Hester A. Kobayashi ◽  
Joseph L. McCarthy ◽  
James W. Patterson ◽  
...  
Keyword(s):  
Panel Discussion ◽  
Genetic Strategies
Sign in / Sign up

Export Citation Format

Share Document