Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test

2020 ◽  
pp. 73-80
Author(s):  
Jessica L. Smith ◽  
Alec J. Hirsch
Keyword(s):  
Zika Virus ◽  
Neutralization Test ◽  
Focus Reduction

scholarly journals A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens

Vaccines ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 66 ◽  
Author(s):  
Frumence ◽  
Viranaicken ◽  
Gadea ◽  
Desprès
Keyword(s):  
Flow Cytometry ◽  
Zika Virus ◽  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Health Concern ◽  
Structural Protein ◽  
Adult Mice ◽  
Gfp Reporter ◽  
Infected Cells ◽  
High Level

Zika virus (ZIKV) is an emerging arthropod-borne virus of major public health concern. ZIKV infection is responsible for congenital Zika disease and other neurological defects. Antibody-mediated virus neutralization is an essential component of protective antiviral immunity against ZIKV. In the present study, we assessed whether our GFP reporter ZIKV derived from African viral strain MR766 could be useful for the development of a flow cytometry neutralization test (FNT), as an alternative to the conventional plaque-reduction neutralization test (PRNT). To improve the efficacy of GFP-expressing MR766, we selected virus variant MR766GFP showing a high level of GFP signal in infected cells. A MR766GFP-based FNT was assayed with immune sera from adult mice that received ZIKBeHMR-2. The chimeric ZIKV clone ZIKBeHMR-2 comprises the structural protein region of epidemic strain BeH819015 into MR766 backbone. We reported that adult mice inoculated with ZIKBeHMR-2 developed high levels of neutralizing anti-ZIKV antibodies. Comparative analysis between MR766GFP-based FNT and conventional PRNT was performed using mouse anti-ZIKBeHMR-2 immune sera. Indistinguishable neutralization patterns were observed when compared with PRNT50 and FNT50. We consider that the newly developed MR766GFP-based FNT is a valid format for measuring ZIKV-neutralizing antibodies in serum specimens.

scholarly journals Retrospective seroepidemiological study of chikungunya infection in South Asia, Southeast Asia and the Pacific region

2016 ◽  
Vol 144 (11) ◽  
pp. 2268-2275 ◽  
Author(s):  
M. M. NGWE TUN ◽  
S. INOUE ◽  
K. Z. THANT ◽  
N. TALEMAITOGA ◽  
A. ARYATI ◽  
...  
Keyword(s):  
Southeast Asia ◽  
South Asia ◽  
Indirect Elisa ◽  
Neutralization Test ◽  
The Philippines ◽  
Asian Countries ◽  
Serum Samples ◽  
Chikv Infection ◽  
The Pacific ◽  
Focus Reduction

SUMMARYChikungunya virus (CHIKV) and Ross River virus (RRV) of the genusAlphavirus, family Togaviridae are mainly transmitted byAedesmosquitoes and the symptoms they cause in patients are similar to dengue. A chikungunya (CHIK) outbreak re-emerged in several Asian countries during 2005–2006. This study aimed to clarify the prevalence of CHIKV infection in suspected dengue patients in six countries in South Asia and Southeast Asia. Seven hundred forty-eight serum samples were from dengue-suspected patients in South Asia and Southeast Asia, and 52 were from patients in Fiji. The samples were analysed by CHIKV IgM capture ELISA, CHIKV IgG indirect ELISA and focus reduction neutralization test against CHIKV or RRV. CHIK-confirmed cases in South Asia, particularly Myanmar and Sri Lanka, were 4·6%, and 6·1%, respectively; and in Southeast Asia, particularly Indonesia, the Philippines and Vietnam, were 27·4%, 26·8% and 25·0%, respectively. It suggests that CHIK was widely spread in these five countries in Asia. In Fiji, no CHIK cases were confirmed; however, RRV-confirmed cases represented 53·6% of suspected dengue cases. It suggests that RRV is being maintained or occasionally entering from neighbouring countries and should be considered when determining a causative agent for dengue-like illness in Fiji.

Development of a focus reduction neutralization test (FRNT) for detection of mumps virus neutralizing antibodies

2010 ◽  
Vol 163 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Sunil R. Vaidya ◽  
David W.G. Brown ◽  
Li Jin ◽  
Dhanraj Samuel ◽  
Nick Andrews ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Mumps Virus ◽  
Focus Reduction

scholarly journals Evaluation of Euroimmun Anti-Zika Virus IgM and IgG Enzyme-Linked Immunosorbent Assays for Zika Virus Serologic Testing

2017 ◽  
Vol 55 (8) ◽  
pp. 2462-2471 ◽  
Author(s):  
Arnaud G. L'Huillier ◽  
Anne Hamid-Allie ◽  
Erik Kristjanson ◽  
Louis Papageorgiou ◽  
Sam Hung ◽  
...  
Keyword(s):  
Public Health ◽  
Immunosorbent Assay ◽  
Zika Virus ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Convenience Sample ◽  
Plaque Reduction Neutralization Test ◽  
Serologic Diagnosis ◽  
Serologic Testing ◽  
Antibody Capture

ABSTRACTWith the emerging Zika virus (ZIKV) epidemic, serologic diagnosis relies on a labor-intensive IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) and confirmation by a plaque reduction neutralization test (PRNT). To streamline serologic testing, several commercial assays have been developed. Our aim was to compare the commercial Euroimmun anti-ZIKV IgM and IgG assays to the reference MAC-ELISA and PRNT currently in use. Serum specimens submitted to Public Health Ontario Laboratory, Canada, were tested for IgM and IgG using the Euroimmun assays and the results were compared with those from MAC-ELISA. The PRNT was performed on positive or equivocal specimens using either MAC-ELISA or Euroimmun assays, MAC-ELISA-inconclusive specimens, and a convenience sample of specimens negative by both assays (cohort 1). Another set of specimens selected on the basis of PRNT results was subsequently tested by the Euroimmun assays (cohort 2). MAC-ELISA was positive, equivocal, negative, and inconclusive in 57/223, 15/223, 147/223, and 4/223 specimens, respectively. Among the 76 specimens that were MAC-ELISA positive, equivocal, or inconclusive, 30 (39.5%) were Euroimmun IgM and/or IgG positive or equivocal. Among the 147 MAC-ELISA-negative specimens, 136 (92.5%) were Euroimmun IgM and IgG negative. The sensitivity of the combined Euroimmun IgM/IgG against the PRNT was 83% (cohort 1) and 92% (cohort 2), whereas the specificity was 81% (cohort 1) and 65% (cohort 2). The combined Euroimmun IgM/IgG showed good specificity (92.5%) but suboptimal sensitivity (39.5%) compared with that of the MAC-ELISA. However, the sensitivity of the combined Euroimmun IgM/IgG against the PRNT was significantly higher (83 to 92%). More studies are needed before commercial assays are implemented for routine ZIKV serologic diagnosis.

scholarly journals Serological divergence of Dobrava and Saaremaa hantaviruses: evidence for two distinct serotypes

2002 ◽  
Vol 128 (1) ◽  
pp. 99-103 ◽  
Author(s):  
K. BRUS SJÖLANDER ◽  
I. GOLOVLJOVA ◽  
V. VASILENKO ◽  
A. PLYUSNIN ◽  
Å. LUNDKVIST
Keyword(s):  
Human Serum ◽  
Neutralization Test ◽  
Serological Relationship ◽  
Serum Samples ◽  
The Balkans ◽  
Human Serum Samples ◽  
End Point ◽  
Focus Reduction ◽  
Relationship Of

In order to investigate the serological relationship of Dobrava hantavirus (DOBV, originating from Slovenia) and the Dobrava-like Saaremaa virus (SAAV, recently discovered in Estonia) we analysed 37 human serum samples, 24 from Estonia and 13 from the Balkans, by focus reduction neutralization test (FRNT). Most of the Estonian sera (19), including all sera from Saaremaa island (12), reacted with higher FRNT end-point titres to the local SAAV; the majority of them (15 and 11, respectively), with at least fourfold or higher titres to SAAV than to DOBV. In contrast, out of the 13 sera collected in Slovenia, Bosnia-Herzegovina and Greece, only one reacted more strongly with SAAV (with a twofold higher titre), while 10 of these sera reacted more strongly with the local DOBV (9/10 with fourfold or higher titres). These results indicate that DOBV and SAAV define unique hantavirus serotypes.

Serological Evidence of Zika Virus Infection in Febrile Patients and Healthy Blood Donors in Sabah, Malaysian Borneo, 2017–2018

2021 ◽  
Author(s):  
Mya Myat Ngwe Tun ◽  
Daisuke Mori ◽  
Shahnaz Binti Sabri ◽  
Omar Kugan ◽  
Saliz Binti Shaharom ◽  
...  
Keyword(s):  
Zika Virus ◽  
Blood Donors ◽  
Neutralization Test ◽  
Febrile Illness ◽  
Healthy Blood Donor ◽  
Serological Evidence ◽  
Serum Samples ◽  
Zikv Infection ◽  
The Caribbean ◽  
Malaysian Borneo

Several Zika virus (ZIKV) seroprevalence studies have been conducted in Africa, Asia, Oceania, the Americas, and the Caribbean. However, studies on ZIKV seroprevalence are limited in Malaysia though several studies have shown that the disease is endemic in the Malaysian state of Sabah. To evaluate the seroprevalence of ZIKV infection, 818 serum samples were collected from febrile patients and healthy blood donors from the Kudat and Kota Kinabalu districts in Sabah from 2017 to 2018. They were screened for ZIKV infection by IgM and IgG ELISA, and positive ZIKV IgM samples were subjected to a 90% neutralization test for confirmation. Twenty-four (6% [95% CI 4 to 8]) confirmed and two (0.5% [95% CI 0.13 to 1.8]) probable ZIKV infections were detected among 400 febrile illness patients. Of 418 healthy blood donor samples, six (1.4% [95% CI 0.65 to 3]) were determined as confirmed ZIKV infections and six (1.4% [95% CI 0.65 to 3]) indicated probable ZIKV infection. This is the first study on the seroprevalence of ZIKV infections among patients and healthy blood donors in Sabah. Compared with previous studies in Malaysia, this study shows that the incidence of ZIKV infection has increased. It also suggests that a sero-surveillance system is essential to determine the circulation of ZIKV in Sabah, Malaysia.

scholarly journals Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection

2018 ◽  
Vol 12 (3) ◽  
pp. e0006342 ◽  
Author(s):  
Andrea Cristine Koishi ◽  
Andréia Akemi Suzukawa ◽  
Camila Zanluca ◽  
Daria Elena Camacho ◽  
Guillermo Comach ◽  
...  
Keyword(s):  
Virus Infection ◽  
High Throughput ◽  
Zika Virus ◽  
Neutralization Test ◽  
Zika Virus Infection

Rapid focus reduction neutralization test of Japanese encephalitis virus in microtiter system

1985 ◽  
Vol 86 (1-2) ◽  
pp. 129-135 ◽  
Author(s):  
Y. Okuno ◽  
T. Fukunaga ◽  
M. Tadano ◽  
Y. Okamoto ◽  
T. Ohnishi ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Neutralization Test ◽  
Encephalitis Virus ◽  
Focus Reduction

scholarly journals Seroprevalence of West Nile Virus in Wild Birds in Far Eastern Russia Using a Focus Reduction Neutralization Test

2011 ◽  
Vol 84 (3) ◽  
pp. 461-465 ◽  
Author(s):  
Ryo Murata ◽  
Ikuo Takashima ◽  
Leonid I. Ivanov ◽  
Kentaro Yoshii ◽  
Kensuke Nakajima ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
Wild Birds ◽  
West Nile ◽  
Far Eastern Russia ◽  
Focus Reduction ◽  
Far Eastern ◽  
Eastern Russia

scholarly journals Evaluation of the Diagnostic Accuracy of a New Biosensors-Based Rapid Diagnostic Test for the Point-Of-Care Diagnosis of Previous and Recent Dengue Infections in Malaysia

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 129
Author(s):  
Zhuo Lin Chong ◽  
Hui Jen Soe ◽  
Amni Adilah Ismail ◽  
Tooba Mahboob ◽  
Samudi Chandramathi ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Neutralization Test ◽  
Point Of Care ◽  
Vaccination Strategy ◽  
Inhibition Assay ◽  
Hemagglutination Inhibition Assay ◽  
Major Threat ◽  
Good Point ◽  
Focus Reduction ◽  
Healthy Participants

Dengue is a major threat to public health globally. While point-of-care diagnosis of acute/recent dengue is available to reduce its mortality, a lack of rapid and accurate testing for the detection of previous dengue remains a hurdle in expanding dengue seroepidemiological surveys to inform its prevention, especially vaccination, to reduce dengue morbidity. This study evaluated ViroTrack Dengue Serostate, a biosensors-based semi-quantitative anti-dengue IgG (immunoglobulin G) immuno-magnetic agglutination assay for the diagnosis of previous and recent dengue in a single test. Blood samples were obtained from 484 healthy participants recruited randomly from two communities in Petaling district, Selangor, Malaysia. The reference tests were Panbio Dengue IgG indirect and capture enzyme-linked immunosorbent assays, in-house hemagglutination inhibition assay, and focus reduction neutralization test. Dengue Serostate had a sensitivity and specificity of 91.1% (95%CI 87.8–93.8) and 91.1% (95%CI 83.8–95.8) for the diagnosis of previous dengue, and 90.2% (95%CI 76.9–97.3) and 93.2% (95%CI 90.5–95.4) for the diagnosis of recent dengue, respectively. Its positive predictive value of 97.5% (95%CI 95.3–98.8) would prevent most dengue-naïve individuals from being vaccinated. ViroTrack Dengue Serostate’s good point-of-care diagnostic accuracy can ease the conduct of dengue serosurveys to inform dengue vaccination strategy and facilitate pre-vaccination screening to ensure safety.

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