Tumor Growth Analysis of Ewing Sarcoma Cell Lines Using Subcutaneous Xenografts in Mice

2020 ◽  
pp. 191-199
Author(s):  
Florencia Cidre-Aranaz ◽  
Shunya Ohmura
Keyword(s):  
Tumor Growth ◽  
Cell Lines ◽  
Ewing Sarcoma ◽  
Growth Analysis ◽  
Sarcoma Cell

Inhibition of proliferation in human MNNG/HOS osteosarcoma and SK-ES-1 Ewing sarcoma cell lines in vitro and in vivo by antagonists of growth hormone-releasing hormone

Cancer ◽  
2002 ◽  
Vol 95 (8) ◽  
pp. 1735-1745 ◽  
Author(s):  
Ryszard Braczkowski ◽  
Andrew V. Schally ◽  
Artur Plonowski ◽  
Jozsef L. Varga ◽  
Kate Groot ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Cell Lines ◽  
Ewing Sarcoma ◽  
Growth Hormone Releasing Hormone ◽  
Sarcoma Cell ◽  
Inhibition Of Proliferation ◽  
Releasing Hormone

scholarly journals Altertoxin II, a Highly Effective and Specific Compound against Ewing Sarcoma

Cancers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 6176
Author(s):  
Andrew J. Robles ◽  
Wentao Dai ◽  
Saikat Haldar ◽  
Hongyan Ma ◽  
Victoria M. Anderson ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Ewing Sarcoma ◽  
Screening Program ◽  
Therapeutic Drug ◽  
G1 Arrest ◽  
Dna Double Strand Breaks ◽  
Sarcoma Cell ◽  
Highly Effective

A screening program designed to identify natural products with selective cytotoxic effects against cell lines representing different types of pediatric solid tumors led to the identification of altertoxin II as a highly potent and selective cytotoxin against Ewing sarcoma cell lines. Altertoxin II, but not the related compounds altertoxin I and alteichin, was highly effective against every Ewing sarcoma cell line tested, with an average 25-fold selectivity for these cells as compared to cells representing other pediatric and adult cancers. Mechanism of action studies revealed that altertoxin II causes DNA double-strand breaks, a rapid DNA damage response, and cell cycle accumulation in the S phase. Our studies also demonstrate that the potent effects of altertoxin II are partially dependent on the progression through the cell cycle, because the G1 arrest initiated by a CDK4/6 inhibitor decreased antiproliferative potency more than 10 times. Importantly, the cell-type-selective DNA-damaging effects of altertoxin II in Ewing sarcoma cells occur independently of its ability to bind directly to DNA. Ultimately, we found that altertoxin II has a dose-dependent in vivo antitumor efficacy against a Ewing sarcoma xenograft, suggesting that it has potential as a therapeutic drug lead and will be useful to identify novel targets for Ewing-sarcoma-specific therapies.

scholarly journals The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes

Cancers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 5668
Author(s):  
Laura García-García ◽  
Enrique Fernández-Tabanera ◽  
Saint T. Cervera ◽  
Raquel M. Melero-Fernández de Mera ◽  
Santiago Josa ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Cell Lines ◽  
Ewing Sarcoma ◽  
Target Genes ◽  
Zinc Finger Protein ◽  
Functional Characterization ◽  
Sarcoma Cell ◽  
Critical Pathways ◽  
Pediatric Tumor ◽  
Sarcoma Cells

Ewing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a specific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify critical pathways involved in Ewing sarcoma tumorigenesis. In this work, we focused on the transcription factors regulated by EWSR1-FLI1 in Ewing sarcoma. Transcriptomic analysis of the Ewing sarcoma cell line A673 indicated that one of the genes more strongly upregulated by EWSR1-FLI1 was FEZF1 (FEZ family zinc finger protein 1), a transcriptional repressor involved in neural cell identity. The functional characterization of FEZF1 was performed in three Ewing sarcoma cell lines (A673, SK-N-MC, SK-ES-1) through an shRNA-directed silencing approach. FEZF1 knockdown inhibited clonogenicity and cell proliferation. Finally, the analysis of the FEZF1-dependent expression profile in A673 cells showed several neural genes regulated by FEZF1 and concomitantly regulated by EWSR1-FLI1. In summary, FEZF1 is transcriptionally regulated by EWSR1-FLI1 in Ewing sarcoma cells and is involved in the regulation of neural-specific genes, which could explain the neural-like phenotype observed in several Ewing sarcoma tumors and cell lines.

scholarly journals Eribulin alone or in combination with the PLK1 inhibitor BI 6727 triggers intrinsic apoptosis in Ewing sarcoma cell lines

Oncotarget ◽  
2017 ◽  
Vol 8 (32) ◽  
pp. 52445-52456 ◽  
Author(s):  
Lilly Magdalena Weiß ◽  
Manuela Hugle ◽  
Simone Fulda
Keyword(s):  
Cell Lines ◽  
Ewing Sarcoma ◽  
Intrinsic Apoptosis ◽  
Sarcoma Cell

Gene Expression Profile of Ewing Sarcoma Cell Lines Differing in Their EWS-FLI1 Fusion Type

2005 ◽  
Vol 27 (10) ◽  
pp. 537-542 ◽  
Author(s):  
Eva Bandr??s ◽  
Raquel Malumbres ◽  
Alvaro Escalada ◽  
Elena Cubedo ◽  
Iranzu Gonz??lez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Cell Lines ◽  
Expression Profile ◽  
Ewing Sarcoma ◽  
Sarcoma Cell ◽  
Fusion Type

Abstract 2034: LSD1 inhibition alone and in combination with chemotherapy in Ewing sarcoma cell lines

2017 ◽  
Author(s):  
Darcy Welch ◽  
Elliot Kahen ◽  
Christopher L. Cubitt ◽  
Damon R. Reed
Keyword(s):  
Cell Lines ◽  
Ewing Sarcoma ◽  
Sarcoma Cell

Suberoylanilide hydroxamic acid synergistically enhances the antitumor activity of etoposide in Ewing sarcoma cell lines

2015 ◽  
Vol 26 (8) ◽  
pp. 843-851 ◽  
Author(s):  
Rebekka Unland ◽  
Dagmar Clemens ◽  
Ulrike Heinicke ◽  
Jenny C. Potratz ◽  
Marc Hotfilder ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Cell Lines ◽  
Ewing Sarcoma ◽  
Hydroxamic Acid ◽  
Suberoylanilide Hydroxamic Acid ◽  
Sarcoma Cell
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