A Luminescence Assay to Quantify Cell Viability in Real Time

2020 ◽  
pp. 187-196
Author(s):  
Peter Hofsteen ◽  
Natasha Karassina ◽  
James J. Cali ◽  
Jolanta Vidugiriene
Keyword(s):  
Cell Viability ◽  
Real Time ◽  
Luminescence Assay

scholarly journals Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging

BMC Biology ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Michael Conway ◽  
Tingting Xu ◽  
Andrew Kirkpatrick ◽  
Steven Ripp ◽  
Gary Sayler ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Viability ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Proliferation And Differentiation ◽  
Real Time Tracking

scholarly journals Decreased miRNA-146A in Glioblastoma Multiforme and Regulation of Cell Proliferation and Apoptosis by Target Notch1

2016 ◽  
Vol 31 (3) ◽  
pp. 270-275 ◽  
Author(s):  
Hong-qi Hu ◽  
Lai-guang Sun ◽  
Wu-jun Guo
Keyword(s):  
Glioblastoma Multiforme ◽  
Cell Viability ◽  
Real Time ◽  
Real Time Pcr ◽  
Glioma Cells ◽  
Viability Analysis ◽  
Nontumor Tissue ◽  
Proliferation And Apoptosis ◽  
A Cell ◽  
The Relationship

Objective The primary purpose of this paper is to investigate the relationship between the microRNA 146a (miR-146a) and the proliferation of cells occurring in glioblastoma multiforme. The secondary purpose of the paper is to investigate abnormalities of expression in miR-146a. Methods A real-time PCR assay was used to investigate the abnormal expression of miR-146a in glioma and adjacent tissue. Lipofection was used to transfect a mimic of miR-146a and induce the upregulation of miR-146a. Real-time PCR was used to observe the expression level of miR-146a. A cell viability analysis was conducted using MTT. A luciferase report vector was used to identify potential targets for miR-146a. Results The miR-146a component was found to be downregulated in glioma tissue compared with adjacent nontumor tissue (p<0.05). The upregulation of miR-146a in glioma cells through miR-146a mimic transfection led to reduction of cell viability and to an increase in the percentage of apoptosis. Notch1 was the name of the potential targeted gene for miR-146a in glioma. Conclusions The study found that the presence of miR-146a potentially affected the proliferation of glioma cells by regulating the rate of Notch1 expression.

scholarly journals Anti-poliovirus activity ofBaccharis dracunculifoliaand propolis by cell viability determination and real-time PCR

2009 ◽  
Vol 107 (5) ◽  
pp. 1669-1680 ◽  
Author(s):  
M.C. Búfalo ◽  
A.S. Figueiredo ◽  
J.P.B. de Sousa ◽  
J.M.G. Candeias ◽  
J.K. Bastos ◽  
...  
Keyword(s):  
Cell Viability ◽  
Real Time ◽  
Real Time Pcr ◽  
Viability Determination

Real-time evaluation of cell viability using nanoprobes

2015 ◽  
Vol 10 (4) ◽  
pp. 391-392
Author(s):  
Marjan Rafat
Keyword(s):  
Cell Viability ◽  
Real Time ◽  
Time Evaluation

scholarly journals Environmental Monitoring of Mycobacterium bovis in Badger Feces and Badger Sett Soil by Real-Time PCR, as Confirmed by Immunofluorescence, Immunocapture, and Cultivation

2007 ◽  
Vol 73 (22) ◽  
pp. 7471-7473 ◽  
Author(s):  
F. P. Sweeney ◽  
O. Courtenay ◽  
V. Hibberd ◽  
R. G. Hewinson ◽  
L. A. Reilly ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Cell Viability ◽  
Real Time ◽  
Species Identification ◽  
Mycobacterium Bovis ◽  
Real Time Pcr ◽  
Immunomagnetic Capture

ABSTRACT Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.

scholarly journals LncRNA FOXD2-AS1 Protects Against Cerebral Ischemia-Reperfusion Injury Through miR-3144-5p/ KCTD15 Axis

2021 ◽  
Author(s):  
Qi Gao ◽  
Yanfeng Wang
Keyword(s):  
Cerebral Ischemia ◽  
Cell Viability ◽  
Reperfusion Injury ◽  
Real Time ◽  
Pc12 Cells ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Luciferase Reporter ◽  
Cerebral Ischemia Reperfusion ◽  
Cerebral Ischemia Reperfusion Injury

Abstract Background: Long non-coding RNAs (lnc-RNAs) and microRNAs (miRNAs) play key roles in the development of stroke. LncRNA FOXD2-AS1 has been reported to be important in many cancers. However, the role of lncRNA FOXD2-AS1 in stroke is limited known.Methods: Real-time polymerase chain reaction (real-time PCR) assays were used to measure expressions of lncRNA FOXD2-AS1, miR-3144-5p and KCTD15. Western blot assays were employed to examine Bax and Bcl2 protein expression. The cell viability was measured by cck8 assay. The cell apoptosis was detected by TUNEL staining assay The interaction between FOXD2-AS1, miR-3144-5p and KCTD was confirmed by site-directed mutagenesis and luciferase assays. Results: The expression of lncRNA FOXD2-AS1 was down-regulated in blood sample from stroke patients and MAO mice tissues. In addition, lncRNA FOXD2-AS1 was decreased on OGD/R treated cells. LncRNA FOXD2-AS1 overexpression promoted cell viability and reduced apoptosis in OGD/R-Induced PC12 Cells. LncRNA FOXD2-AS1 could interact with miR-3144-5p. Meanwhile, inhibition of miR-3144-5p alleviated OGD/ R-induced neuronal injury in PC12 Cells. Dual luciferase reporter assay verified that KCTD15 was a target of miR-3144-5p. And level of KCTD15 was positive with LncRNA FOXD2-AS1.Conclusion: Taken together, lncRNA FOXD2-AS1 protected against c cerebral ischemia-reperfusion injury by acting as a sponge of miR-3144-5p to modulate KCTD15 level.

317. DIFFERENTIAL EFFECTS OF cAMP AND CRH IN CELL VIABILITY AND CELL FUSION OF CYTOTROPHOBLASTIC CELLS

2010 ◽  
Vol 22 (9) ◽  
pp. 117
Author(s):  
Y. Chen ◽  
M. Allars ◽  
C. Abou-Seif ◽  
R. Smith ◽  
R. C. Nicholson
Keyword(s):  
Gene Expression ◽  
Cell Viability ◽  
Real Time ◽  
Cell Fusion ◽  
Fas Ligand ◽  
Bewo Cell ◽  
Rt Pcr ◽  
Cytotrophoblast Cell ◽  
Gene Assay ◽  
Hcg Secretion

Cell fusion of cytotrophoblasts is critical for a successful pregnancy, and is marked biochemically by proteins such as human chorionic gonadotrophin (hCG) and syncytin, and morphologically as large multi-nucleated cells. Placental corticotrophin releasing hormone (CRH) plays diverse roles during pregnancy and has been linked to the length of gestation. It is well accepted that CRH expression could be upregulated by some hormones, such as hCG via cellular cAMP, and that trophoblastic cell fusion can be induced by Forskolin (increases cAMP). We have shown, by reporter gene assay in human primary placental cells, that 8-Br-cAMP increased CRH gene expression. Thus, it is of interest to determine the roles of 8-Br-cAMP and CRH on trophoblast cell viability and cell fusion. Treatment with 8-Br-cAMP for 72 h significantly inhibited BeWo cell viability (MTT assay) by 29.7% (50 μM) and 54.8% (100 μM), and increased both apoptotic and necrotic cells (FACS analysis). 8-Br-cAMP (100 μM, 48 h) resulted in the appearance of nuclear fragments (DAPI stain) and Fas ligand gene expression by 6.8-fold (real-time RT-PCR). Syncytin 1 mRNA increased by 3.2-fold (real-time RT-PCR) and hCG secretion increased by 4-fold (ELISA assay). The formation of syncytium (CellMask and Hoechst co-stain) could clearly be seen by 72 h. CRH mRNA increased with 8-Br-cAMP treatment of BeWo cells (15-fold at 48 h). Treatment with CRH (100 nM) had a mild inhibitory effect on cell growth (~18%) but no effect on cell viability, up-regulated the expression of syncytin 1 mRNA (2.3-fold at 48 h) and induced cell fusion (72 h), but had no effect on hCG secretion. In summary, we show that 8-Br-cAMP and CRH are both potential inducers of cytotrophoblast cell fusion, and there is a dissociation between morphological and biochemical differentiation. Our data also indicates that the process of cell fusion can be associated with both apoptotic and non-apoptotic events.

Reduced Graphene Oxide-Based Assay for Real-Time Monitoring of Cancer Cell Viability

NANO ◽  
2015 ◽  
Vol 10 (07) ◽  
pp. 1550094
Author(s):  
Jing Wu ◽  
Yang Tang ◽  
Zengliang Shi ◽  
Zhengshan Tian ◽  
Junhua Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cell Viability ◽  
Cancer Cells ◽  
Real Time ◽  
Reduced Graphene Oxide ◽  
Cancer Cell ◽  
Electronic Device ◽  
Physiological State ◽  
Important Indicator ◽  
Reduced Graphene

Viability of cancer cell is an important indicator of physiological state and function of cells, which can be effected by the change of pH in the medium solution, due to the increase of carbon oxide and lactic acid caused by respiration. Although many methods have been developed to detect the viability of cells, mostly based on cytochemical staining and polymerase chain reaction (PCR) technology are time consuming. In this paper, an electronic device was made by thermal reduced graphene oxide (RGO) for detection of cancer cell viability in real-time. This electronic device could be used to monitor the metabolic activity and viability of cancer cells based on the change in pH value. As the pH decreases, colon cancer cells loose viability and the current decreases. This RGO device is simple, sensitive and label-free and could serve as a platform for detection of cells and drug testing.

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