Environmental Monitoring of Mycobacterium bovis in Badger Feces and Badger Sett Soil by Real-Time PCR, as Confirmed by Immunofluorescence, Immunocapture, and Cultivation

ABSTRACT Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.

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Rapid Species Identification of Cooked Poisonous Mushrooms by Using Real-Time PCR

Applied and Environmental Microbiology ◽

10.1128/aem.02082-07 ◽

2008 ◽

Vol 74 (10) ◽

pp. 3306-3309 ◽

Cited By ~ 24

Author(s):

Kazuhiko Maeta ◽

Tomoya Ochi ◽

Keisuke Tokimoto ◽

Norihiro Shimomura ◽

Nitaro Maekawa ◽

...

Keyword(s):

Real Time ◽

Species Identification ◽

Real Time Pcr ◽

Specific Test ◽

Specific Identification ◽

Specific Fluorescence ◽

Species Specific ◽

Poisonous Mushrooms

ABSTRACT Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.

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Validation of real-time PCR technique for detection of Mycobacterium bovis and Brucella abortus in bovine raw milk

Brazilian Journal of Microbiology ◽

10.1007/s42770-020-00319-9 ◽

2020 ◽

Vol 51 (4) ◽

pp. 2095-2100

Author(s):

Débora R. Mascarenhas ◽

David Germano G. Schwarz ◽

Antônio A. Fonseca Júnior ◽

Tatiana F.P. Oliveira ◽

Maria A.S. Moreira

Keyword(s):

Real Time ◽

Mycobacterium Bovis ◽

Brucella Abortus ◽

Real Time Pcr ◽

Raw Milk

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Decreased miRNA-146A in Glioblastoma Multiforme and Regulation of Cell Proliferation and Apoptosis by Target Notch1

The International Journal of Biological Markers ◽

10.5301/jbm.5000194 ◽

2016 ◽

Vol 31 (3) ◽

pp. 270-275 ◽

Cited By ~ 8

Author(s):

Hong-qi Hu ◽

Lai-guang Sun ◽

Wu-jun Guo

Keyword(s):

Glioblastoma Multiforme ◽

Cell Viability ◽

Real Time ◽

Real Time Pcr ◽

Glioma Cells ◽

Viability Analysis ◽

Nontumor Tissue ◽

Proliferation And Apoptosis ◽

A Cell ◽

The Relationship

Objective The primary purpose of this paper is to investigate the relationship between the microRNA 146a (miR-146a) and the proliferation of cells occurring in glioblastoma multiforme. The secondary purpose of the paper is to investigate abnormalities of expression in miR-146a. Methods A real-time PCR assay was used to investigate the abnormal expression of miR-146a in glioma and adjacent tissue. Lipofection was used to transfect a mimic of miR-146a and induce the upregulation of miR-146a. Real-time PCR was used to observe the expression level of miR-146a. A cell viability analysis was conducted using MTT. A luciferase report vector was used to identify potential targets for miR-146a. Results The miR-146a component was found to be downregulated in glioma tissue compared with adjacent nontumor tissue (p<0.05). The upregulation of miR-146a in glioma cells through miR-146a mimic transfection led to reduction of cell viability and to an increase in the percentage of apoptosis. Notch1 was the name of the potential targeted gene for miR-146a in glioma. Conclusions The study found that the presence of miR-146a potentially affected the proliferation of glioma cells by regulating the rate of Notch1 expression.

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Anti-poliovirus activity ofBaccharis dracunculifoliaand propolis by cell viability determination and real-time PCR

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2009.04354.x ◽

2009 ◽

Vol 107 (5) ◽

pp. 1669-1680 ◽

Cited By ~ 43

Author(s):

M.C. Búfalo ◽

A.S. Figueiredo ◽

J.P.B. de Sousa ◽

J.M.G. Candeias ◽

J.K. Bastos ◽

...

Keyword(s):

Cell Viability ◽

Real Time ◽

Real Time Pcr ◽

Viability Determination

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Species Identification of Fox-, Mink-, Dog-, and Rabbit-Derived Ingredients by Multiplex PCR and Real-Time PCR Assay

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-017-2621-2 ◽

2017 ◽

Vol 185 (1) ◽

pp. 1-12 ◽

Cited By ~ 10

Author(s):

Qingqing Wu ◽

Shengnan Xiang ◽

Wenjun Wang ◽

Jinyan Zhao ◽

Jinhua Xia ◽

...

Keyword(s):

Multiplex Pcr ◽

Real Time ◽

Species Identification ◽

Real Time Pcr ◽

Pcr Assay

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Detection and species identification of microsporidial infections using SYBR Green real-time PCR

Journal of Medical Microbiology ◽

10.1099/jmm.0.026781-0 ◽

2011 ◽

Vol 60 (4) ◽

pp. 459-466 ◽

Cited By ~ 29

Author(s):

Spencer D. Polley ◽

Samuel Boadi ◽

Julie Watson ◽

Alan Curry ◽

Peter L. Chiodini

Keyword(s):

Real Time ◽

Species Identification ◽

Real Time Pcr ◽

Simultaneous Detection ◽

Enterocytozoon Bieneusi ◽

Sybr Green ◽

Pcr Assay ◽

Real Time Analysis ◽

Highly Sensitive ◽

Stool Samples

Diagnosis of microsporidial infections is routinely performed by light microscopy, with unequivocal non-molecular species identification achievable only through electron microscopy. This study describes a single SYBR Green real-time PCR assay for the simultaneous detection and species identification of such infections. This assay was highly sensitive, routinely detecting infections containing 400 parasites (g stool sample)−1, whilst species identification was achieved by differential melt curves on a Corbett Life Science Rotor-Gene 3000. A modification of the QIAamp DNA tissue extraction protocol allowed the semi-automated extraction of DNA from stools for the routine diagnosis of microsporidial infection by real-time PCR. Of 168 stool samples routinely analysed for microsporidian spores, only five were positive by microscopy. By comparison, 17 were positive for microsporidial DNA by real-time analysis, comprising 14 Enterocytozoon bieneusi, one Encephalitozoon cuniculi and two separate Pleistophora species infections.

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