Doubled Haploidy for Cow Cockle (Saponaria vaccaria L.)

2021 ◽  
pp. 263-270
Author(s):  
Alison M. R. Ferrie ◽  
Zoë Ehlert
Keyword(s):  
Doubled Haploidy

scholarly journals Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives

2022 ◽  
Vol 12 ◽  
Author(s):  
Brett Hale ◽  
Alison M. R. Ferrie ◽  
Sreekala Chellamma ◽  
J. Pon Samuel ◽  
Gregory C. Phillips
Keyword(s):  
Cell Fate ◽  
Trichostatin A ◽  
Crop Improvement ◽  
Past Research ◽  
Culture Optimization ◽  
Protocol Development ◽  
Doubled Haploidy ◽  
Deacetylase Inhibitor ◽  
Cycle Regulation ◽  
External Stimuli

Androgenesis, which entails cell fate redirection within the microgametophyte, is employed widely for genetic gain in plant breeding programs. Moreover, androgenesis-responsive species provide tractable systems for studying cell cycle regulation, meiotic recombination, and apozygotic embryogenesis within plant cells. Past research on androgenesis has focused on protocol development with emphasis on temperature pretreatments of donor plants or floral buds, and tissue culture optimization because androgenesis has different nutritional requirements than somatic embryogenesis. Protocol development for new species and genotypes within responsive species continues to the present day, but slowly. There is more focus presently on understanding how protocols work in order to extend them to additional genotypes and species. Transcriptomic and epigenetic analyses of induced microspores have revealed some of the cellular and molecular responses required for or associated with androgenesis. For example, microRNAs appear to regulate early microspore responses to external stimuli; trichostatin-A, a histone deacetylase inhibitor, acts as an epigenetic additive; ά-phytosulfokine, a five amino acid sulfated peptide, promotes androgenesis in some species. Additionally, present work on gene transfer and genome editing in microspores suggest that future endeavors will likely incorporate greater precision with the genetic composition of microspores used in doubled haploid breeding, thus likely to realize a greater impact on crop improvement. In this review, we evaluate basic breeding applications of androgenesis, explore the utility of genomics and gene editing technologies for protocol development, and provide considerations to overcome genotype specificity and morphogenic recalcitrance in non-model plant systems.

The usefulness and limitations of estimating the number of genes in a barley breeding programme

1985 ◽  
Vol 105 (2) ◽  
pp. 285-290 ◽  
Author(s):  
W. Powell ◽  
P. D. S. Caligari ◽  
J. L. Jinks
Keyword(s):  
Inbred Lines ◽  
Breeding Programme ◽  
Single Seed Descent ◽  
Effective Factors ◽  
Barley Breeding ◽  
The Past ◽  
Doubled Haploidy ◽  
Quantitative Characters ◽  
Effective Factor ◽  
Number Of Genes

SUMMARYRandom inbred lines produced by doubled haploidy and single seed descent have been used to estimate the number of genes or more correctly effective factors (k) controlling quantitative characters in barley. Estimates of k obtained by various biometrical methods are generally an underestimate since the effective factor may be considered a unit only in a temporary sense. In the past, estimates of k have been used to predict the range of inbreds extractable from a cross. Alternative and efficient methods are now available to predict the number of inbreds expected to exceed any given standard. Under these circumstances the need to estimate the number of genes is questionable.

Doubled haploidy and induced mutation

Euphytica ◽  
2006 ◽  
Vol 158 (3) ◽  
pp. 359-370 ◽  
Author(s):  
I. Szarejko ◽  
B. P. Forster
Keyword(s):  
Induced Mutation ◽  
Doubled Haploidy

New frontiers in doubled haploidy breeding in wheat

2015 ◽  
Vol 52 (4) ◽  
pp. 1 ◽  
Author(s):  
Harinder K Chaudhary ◽  
Anila Badiyal ◽  
Navdeep S Jamwal
Keyword(s):  
Doubled Haploidy

Inheritance of the blue aleurone trait in diverse wheat crosses

Genome ◽  
1990 ◽  
Vol 33 (4) ◽  
pp. 525-529 ◽  
Author(s):  
V. D. Keppenne ◽  
P. S. Baenziger
Keyword(s):  
Triticum Aestivum ◽  
Genetic Marker ◽  
Dominant Gene ◽  
Triticum Aestivum L ◽  
Progeny Testing ◽  
Seed Color ◽  
Male Sterile ◽  
Agropyron Elongatum ◽  
Doubled Haploidy ◽  
Wheat Lines

The blue aleurone trait has been suggested as a useful genetic marker in wheat (Triticum aestivum L.). However, little information is available on its transmission in diverse backgrounds and on its use to identify hybrid seed. UC66049, a hexaploid spring wheat with a spontaneous translocation that included the gene for the blue aleurone trait (Ba) from Agropyron elongatum (Host) P.B. (synonymous with Elytrigia pontica (Podp.) Holub), was crossed to seven wheat cultivars to test the transmission of the trait. UC66049 was crossed to male-sterile red wheat lines to evaluate the blue aleurone trait as a marker for confirming hybridity. Ba segregated as a dominant gene that was transmitted normally through the male and female gametes. For 6 of 7 crosses with diverse pedigrees, we experienced problems with misclassification of the aleurone color in the F2 seed generation, determined by the F3 seed family data. The blue aleurone trait is a good genetic marker; however, progeny testing may be needed to confirm the F2 genotypes in some environments or genetic backgrounds. Moreover, Ba is useful in determining the amount of controlled hybridity as opposed to self-fertility and (or) outcrossing in genetic male-sterile wheat lines. The use of Ba to confirm doubled haploidy was proposed.Key words: Agropyron elongatum, seed color, genetics, Triticum aestivum, Elytrigia pontica.

scholarly journals New frontiers in chromosome elimination-mediated doubled haploidy breeding: Focus on speed breeding in bread and durum wheat

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
H. K. Chaudhary ◽  
P. K. Sharma ◽  
N. V. ManoJ ◽  
K. Singh
Keyword(s):  
Durum Wheat ◽  
Chromosome Elimination ◽  
Rectovaginal Septum ◽  
Perineal Body ◽  
Birth Canal ◽  
Doubled Haploidy ◽  
Perineal Lacerations

Rectovaginal lacerations in the mare occur during parturition when the foal’s limb(s) or head are forced caudal and dorsal. The injury is seen predominantly in primiparous mares and is usually due to violent expulsive efforts by the mare (Colbern et al., 1985; Turner and McIlwraith, 1989). The injury is also seen following forced extraction of a large fetus or extraction before full dilation of the birth canal. Third-degree perineal lacerations occur when there is tearing through the rectovaginal septum, the musculature of the rectum and vagina, and the perineal body.

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