Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

2021 ◽  
pp. 129-140
Author(s):  
Maren Schubert ◽  
Manfred Nimtz ◽  
Federico Bertoglio ◽  
Stefan Schmelz ◽  
Peer Lukat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Transient Gene Expression ◽  
Mammalian Proteins

scholarly journals Efficient production of antibody Fab fragment by transient gene expression in insect cells

2017 ◽  
Vol 124 (2) ◽  
pp. 221-226 ◽  
Author(s):  
Keita Mori ◽  
Hirotsugu Hamada ◽  
Takafumi Ogawa ◽  
Yuki Ohmuro-Matsuyama ◽  
Tomohisa Katsuda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Transient Gene Expression ◽  
Efficient Production ◽  
Fab Fragment

Production of an antibody fragment by transient gene expression in insect cells

2014 ◽  
Vol 31 ◽  
pp. S186
Author(s):  
Hideki Yamaji ◽  
Tomohisa Katsuda ◽  
Hirotsugu Hamada ◽  
Keita Mori
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Transient Gene Expression ◽  
Antibody Fragment

Efficient antibody production by transient gene expression in insect cells

2016 ◽  
Vol 33 ◽  
pp. S200-S201
Author(s):  
Hideki Yamaji ◽  
Keita Mori ◽  
Hirotsugu Hamada ◽  
Yuki Ohmuro-Matsuyama ◽  
Tomohisa Katsuda
Keyword(s):  
Gene Expression ◽  
Antibody Production ◽  
Insect Cells ◽  
Transient Gene Expression

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N -Propionyl Groups

2012 ◽  
Vol 12 (5) ◽  
pp. 628-636 ◽  
Author(s):  
Zuzana Kadlecova ◽  
Sophie Nallet ◽  
David L. Hacker ◽  
Lucia Baldi ◽  
Harm-Anton Klok ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Weight ◽  
Large Scale ◽  
Transient Gene Expression

scholarly journals Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

2008 ◽  
Vol 24 (2) ◽  
pp. 458-465 ◽  
Author(s):  
S. Wulhfard ◽  
S. Tissot ◽  
S. Bouchet ◽  
J. Cevey ◽  
M. DeJesus ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chinese Hamster Ovary ◽  
Mild Hypothermia ◽  
Chinese Hamster Ovary Cells ◽  
Transient Gene Expression ◽  
Chinese Hamster ◽  
Ovary Cells
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