Transient gene expression in rose petals via Agrobacterium infiltration

2010 ◽  
Vol 102 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Aneela Yasmin ◽  
Thomas Debener
Keyword(s):  
Gene Expression ◽  
Transient Gene Expression ◽  
Rose Petals

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N -Propionyl Groups

2012 ◽  
Vol 12 (5) ◽  
pp. 628-636 ◽  
Author(s):  
Zuzana Kadlecova ◽  
Sophie Nallet ◽  
David L. Hacker ◽  
Lucia Baldi ◽  
Harm-Anton Klok ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Weight ◽  
Large Scale ◽  
Transient Gene Expression

scholarly journals Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

2008 ◽  
Vol 24 (2) ◽  
pp. 458-465 ◽  
Author(s):  
S. Wulhfard ◽  
S. Tissot ◽  
S. Bouchet ◽  
J. Cevey ◽  
M. DeJesus ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chinese Hamster Ovary ◽  
Mild Hypothermia ◽  
Chinese Hamster Ovary Cells ◽  
Transient Gene Expression ◽  
Chinese Hamster ◽  
Ovary Cells

Transient gene expression optimization and expression vector comparison to improve HIV-1 VLP production in HEK293 cell lines

2017 ◽  
Vol 102 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Javier Fuenmayor ◽  
Laura Cervera ◽  
Sonia Gutiérrez-Granados ◽  
Francesc Gòdia
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Expression Vector ◽  
Hek293 Cell ◽  
Transient Gene Expression ◽  
Expression Optimization ◽  
Hiv 1

Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat

1989 ◽  
Vol 13 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Bruce Lee ◽  
Kate Murdoch ◽  
Jennifer Topping ◽  
Martin Kreis ◽  
Michael G. K. Jones
Keyword(s):  
Gene Expression ◽  
Transient Gene Expression
Sign in / Sign up

Export Citation Format

Share Document