Low-Cost Cell-Surface-Mimic Analysis of Ligand Interactions of Biotinylated Immune Receptors Using Surface Plasmon Resonance

2021 ◽  
pp. 21-35
Author(s):  
Kimiko Kuroki ◽  
Hideo Fukuhara ◽  
Takashi Tadokoro ◽  
Katsumi Maenaka
Keyword(s):  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Low Cost ◽  
Immune Receptors ◽  
Ligand Interactions

scholarly journals Microstructured Surface Plasmon Resonance Sensor Based on Inkjet 3D Printing Using Photocurable Resins with Tailored Refractive Index

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2518
Author(s):  
Nunzio Cennamo ◽  
Lorena Saitta ◽  
Claudio Tosto ◽  
Francesco Arcadio ◽  
Luigi Zeni ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
3D Printing ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Optical Sensor ◽  
Low Cost ◽  
3D Print ◽  
Spr Sensor ◽  
Resonance Sensor ◽  
3D Printed

In this work, a novel approach to realize a plasmonic sensor is presented. The proposed optical sensor device is designed, manufactured, and experimentally tested. Two photo-curable resins are used to 3D print a surface plasmon resonance (SPR) sensor. Both numerical and experimental analyses are presented in the paper. The numerical and experimental results confirm that the 3D printed SPR sensor presents performances, in term of figure of merit (FOM), very similar to other SPR sensors made using plastic optical fibers (POFs). For the 3D printed sensor, the measured FOM is 13.6 versus 13.4 for the SPR-POF configuration. The cost analysis shows that the 3D printed SPR sensor can be manufactured at low cost (∼15 €) that is competitive with traditional sensors. The approach presented here allows to realize an innovative SPR sensor showing low-cost, 3D-printing manufacturing free design and the feasibility to be integrated with other optical devices on the same plastic planar support, thus opening undisclosed future for the optical sensor systems.

scholarly journals An Enhanced Plastic Optical Fiber-Based Surface Plasmon Resonance Sensor with a Double-Sided Polished Structure

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1516
Author(s):  
Lian Liu ◽  
Shijie Deng ◽  
Jie Zheng ◽  
Libo Yuan ◽  
Hongchang Deng ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Low Cost ◽  
Fiber Axis ◽  
Plastic Optical Fiber ◽  
Au Film ◽  
Spr Sensor ◽  
Resonance Sensor

An enhanced plastic optical fiber (POF)-based surface plasmon resonance (SPR) sensor is proposed by employing a double-sided polished structure. The sensor is fabricated by polishing two sides of the POF symmetrically along with the fiber axis, and a layer of Au film is deposited on each side of the polished region. The SPR can be excited on both polished surfaces with Au film coating, and the number of light reflections will be increased by using this structure. The simulation and experimental results show that the proposed sensor has an enhanced SPR effect. The visibility and full width at half maximum (FWHM) of spectrum can be improved for the high measured refractive index (RI). A sensitivity of 4284.8 nm/RIU is obtained for the double-sided POF-based SPR sensor when the measured liquid RI is 1.42. The proposed SPR sensor is easy fabrication and low cost, which can provide a larger measurement range and action area to the measured samples, and it has potential application prospects in the oil industry and biochemical sensing fields.

scholarly journals Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 130 ◽  
Author(s):  
Georgina Ross ◽  
Maria Bremer ◽  
Jan Wichers ◽  
Aart van Amerongen ◽  
Michel Nielen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Speed ◽  
Selection Process ◽  
Low Cost ◽  
Real Life ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Label Free

Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

Antibody–Ligand Interactions for Hydrophobic Charge-Induction Chromatography: A Surface Plasmon Resonance Study

Langmuir ◽  
2015 ◽  
Vol 31 (11) ◽  
pp. 3422-3430 ◽  
Author(s):  
Fang Cheng ◽  
Ming-Yang Li ◽  
Han-Qi Wang ◽  
Dong-Qiang Lin ◽  
Jing-Ping Qu
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Charge Induction ◽  
Ligand Interactions ◽  
Hydrophobic Charge Induction Chromatography

Real-Time Analysis of Ligand-Induced Cell Surface and Intracellular Reactions of Living Mast Cells Using a Surface Plasmon Resonance-Based Biosensor

2002 ◽  
Vol 302 (1) ◽  
pp. 28-37 ◽  
Author(s):  
Michihiro Hide ◽  
Tomoko Tsutsui ◽  
Hajime Sato ◽  
Tomoaki Nishimura ◽  
Kenichi Morimoto ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Mast Cells ◽  
Cell Surface ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Time Analysis ◽  
Real Time Analysis

scholarly journals Dynamic Dispersal of Surface Layer Biofilm Induced by Nanosized TiO 2 Based on Surface Plasmon Resonance and Waveguide

2018 ◽  
Vol 84 (9) ◽  
Author(s):  
Peng Zhang ◽  
Jin-Song Guo ◽  
Peng Yan ◽  
You-Peng Chen ◽  
Wei Wang ◽  
...  
Keyword(s):  
Surface Layer ◽  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Bacterial Attachment ◽  
Short Period ◽  
Bacterial Migration ◽  
Adhesive Ability ◽  
Biofilm Dispersal

ABSTRACT Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO 2 (n-TiO 2 ) on the average thicknesses of Bacillus subtilis biofilm and on bacterial attachment on different surfaces were investigated. The binding mechanism of n-TiO 2 to the cell surface was also probed. The results revealed that n-TiO 2 caused biofilm dispersal and the thicknesses decreased by 2.0 to 2.6 μm after several hours of exposure. The attachment abilities of bacteria with extracellular polymeric substances (EPS) on hydrophilic surfaces were significantly reduced by 31% and 81% under 10 and 100 mg/liter of n-TiO 2 , respectively, whereas those of bacteria without EPS were significantly reduced by 43% and 87%, respectively. The attachment abilities of bacteria with and without EPS on hydrophobic surfaces were significantly reduced by 50% and 56%, respectively, under 100 mg/liter of n-TiO 2 . The results demonstrated that biofilm dispersal can be attributed to the changes in the cell surface structure and the reduction of microbial attachment ability. IMPORTANCE Nanoparticles can penetrate into the outer layer of biofilm in a relatively short period and can bind onto EPS and bacterial surfaces. The current work probed the effects of nanosized TiO 2 (n-TiO 2 ) on biofilm thickness, bacterial migration, and surface properties of the cell in the early stage using the surface plasmon resonance waveguide mode. The results demonstrated that n-TiO 2 decreased the adhesive ability of both cell and EPS and induced bacterial migration and biofilm detachment in several hours. The decreased adhesive ability of microbes and EPS worked against microbial aggregation, reducing the effluent quality in the biological wastewater treatment process.

scholarly journals A Methodology for Porcine Circovirus 2 (PCV-2) Quantification Based on Gold Nanoparticles

Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1087
Author(s):  
Caroline R. Basso ◽  
Taís F. Cruz ◽  
Bruna L. Silva ◽  
Valber A. Pedrosa ◽  
João P. Araújo Junior
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Low Cost ◽  
Porcine Circovirus ◽  
Localized Surface Plasmon ◽  
Transmission Electron ◽  
Porcine Circovirus 2 ◽  
Assembled Monolayers

The aim of the current study is to introduce a methodology aimed at producing a biosensor that uses gold nanoparticles (AuNPs) to detect porcine circovirus 2 (PCV-2). This biosensor was based on AuNPs, which were modified with self-assembled monolayers (SAMs) and antibodies. The AuNPs’ surface and virus modification process applied to enable antibody binding was accompanied by localized surface plasmon resonance (LSPR), surface plasmon resonance (SPR), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). Virus quantification was possible by the light absorption difference in the spectrum at concentrations of 105, 106, 107, 108, and 109 DNA copies/mL PCV-2 in relation to quantitative PCR (qPCR), with an R2 value >0.98. The visualization of colorimetric changes in the different PCV-2 concentrations was possible without the use of equipment. The biosensor production methodology presented reproducibility and specificity, as well as easy synthesis and low cost. An enhanced version of it may be used in the future to replace traditional tests such as PCR.

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