Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

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Microstructured Surface Plasmon Resonance Sensor Based on Inkjet 3D Printing Using Photocurable Resins with Tailored Refractive Index

Polymers ◽

10.3390/polym13152518 ◽

2021 ◽

Vol 13 (15) ◽

pp. 2518

Author(s):

Nunzio Cennamo ◽

Lorena Saitta ◽

Claudio Tosto ◽

Francesco Arcadio ◽

Luigi Zeni ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

3D Printing ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Optical Sensor ◽

Low Cost ◽

3D Print ◽

Spr Sensor ◽

Resonance Sensor ◽

3D Printed

In this work, a novel approach to realize a plasmonic sensor is presented. The proposed optical sensor device is designed, manufactured, and experimentally tested. Two photo-curable resins are used to 3D print a surface plasmon resonance (SPR) sensor. Both numerical and experimental analyses are presented in the paper. The numerical and experimental results confirm that the 3D printed SPR sensor presents performances, in term of figure of merit (FOM), very similar to other SPR sensors made using plastic optical fibers (POFs). For the 3D printed sensor, the measured FOM is 13.6 versus 13.4 for the SPR-POF configuration. The cost analysis shows that the 3D printed SPR sensor can be manufactured at low cost (∼15 €) that is competitive with traditional sensors. The approach presented here allows to realize an innovative SPR sensor showing low-cost, 3D-printing manufacturing free design and the feasibility to be integrated with other optical devices on the same plastic planar support, thus opening undisclosed future for the optical sensor systems.

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Plasma enhanced label-free immunoassay for alpha-fetoprotein based on a U-bend fiber-optic LSPR biosensor

RSC Advances ◽

10.1039/c5ra02910d ◽

2015 ◽

Vol 5 (31) ◽

pp. 23990-23998 ◽

Cited By ~ 37

Author(s):

Gaoling Liang ◽

Zhongjun Zhao ◽

Yin Wei ◽

Kunping Liu ◽

Wenqian Hou ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Localized Surface Plasmon Resonance ◽

Fiber Optic ◽

Cost Effective ◽

Alpha Fetoprotein ◽

Localized Surface Plasmon ◽

Label Free

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).

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Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

Engineering Proceedings ◽

10.3390/i3s2021dresden-10136 ◽

2021 ◽

Vol 6 (1) ◽

pp. 26

Author(s):

Rahat Morad Talukder ◽

Al Shahriar Hossain Rakib ◽

Julija Skolnik ◽

Zohair Usfoor ◽

Katharina Kaufmann ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Nano Particles ◽

Label Free ◽

Specific Detection ◽

Virus Like Particles ◽

Low Concentrations ◽

Challenging Tasks ◽

Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

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An Enhanced Plastic Optical Fiber-Based Surface Plasmon Resonance Sensor with a Double-Sided Polished Structure

Sensors ◽

10.3390/s21041516 ◽

2021 ◽

Vol 21 (4) ◽

pp. 1516

Author(s):

Lian Liu ◽

Shijie Deng ◽

Jie Zheng ◽

Libo Yuan ◽

Hongchang Deng ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Optical Fiber ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Low Cost ◽

Fiber Axis ◽

Plastic Optical Fiber ◽

Au Film ◽

Spr Sensor ◽

Resonance Sensor

An enhanced plastic optical fiber (POF)-based surface plasmon resonance (SPR) sensor is proposed by employing a double-sided polished structure. The sensor is fabricated by polishing two sides of the POF symmetrically along with the fiber axis, and a layer of Au film is deposited on each side of the polished region. The SPR can be excited on both polished surfaces with Au film coating, and the number of light reflections will be increased by using this structure. The simulation and experimental results show that the proposed sensor has an enhanced SPR effect. The visibility and full width at half maximum (FWHM) of spectrum can be improved for the high measured refractive index (RI). A sensitivity of 4284.8 nm/RIU is obtained for the double-sided POF-based SPR sensor when the measured liquid RI is 1.42. The proposed SPR sensor is easy fabrication and low cost, which can provide a larger measurement range and action area to the measured samples, and it has potential application prospects in the oil industry and biochemical sensing fields.

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Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors

Sensors ◽

10.3390/s21134348 ◽

2021 ◽

Vol 21 (13) ◽

pp. 4348

Author(s):

Piotr Mrozek ◽

Ewa Gorodkiewicz ◽

Paweł Falkowski ◽

Bogusław Hościło

Keyword(s):

Refractive Index ◽

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Real Life ◽

Reflectance Measurement ◽

Near Surface ◽

Bimetallic Surface ◽

Calibration Curves ◽

Surface Sensing

Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the results of experimental reflectance measurement of real-life systems, were used for the analysis of these sensors. Mutual relationships were determined between ∂Y/∂n components of sensitivity of the Y signal in the SPR measurement to change the refractive index n of the near-surface sensing layer and ∂n/∂c sensitivity of refractive index n to change the analyte’s concentration, c, for both types of sensors. Obtained results were related to experimentally determined calibration curves of both sensors. A characteristic feature arising from the comparison of calibration curves is the similar level of Au and Ag–Au biosensors’ sensitivity in the linear range, where the signal of the AgAu sensor is at a level several times greater. It was shown that the influence of sensing surface morphology on the ∂n/∂c sensitivity component had to be incorporated to explain the features of calibration curves of sensors. The shape of the sensory surface relief was proposed to increase the sensor sensitivity at low analyte concentrations.

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Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay

World Mycotoxin Journal ◽

10.3920/wmj2011.1351 ◽

2012 ◽

Vol 5 (2) ◽

pp. 117-126 ◽

Cited By ~ 18

Author(s):

J.P. Meneely ◽

J.G. Quinn ◽

E.M. Flood ◽

J. Hajšlová ◽

C.T. Elliott

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

High Capacity ◽

Cross Reactivity ◽

Baby Food ◽

Breakfast Cereal ◽

Screening Assay ◽

Coefficients Of Variation ◽

Assay Precision

This manuscript describes a rapid surface plasmon resonance (SPR) immunoassay for the simultaneous determination of the sum of T-2/HT-2 toxins (T-2/HT-2) and deoxynivalenol (DON), in cereals and cereal-based products. The assay is based on an inhibition format employing a monoclonal antibody raised against HT-2 with cross reactivity to T-2 and a polyclonal antibody raised against DON, thereby enabling the detection of the three trichothecene mycotoxins (types A and B). The surface chemistry involved an equal mixture of HT-2 and DON covalently coupled onto a high capacity COOH5 sensor chip. Using the specified antibodies and a mixed toxin sensor surface, and running calibration curves (HT-2 and DON) and samples in parallel it has been proven that it is feasible to develop a multiplex assay on this SPR platform. In-house validation has shown limits of detection of 12, 1 and 29 μg/kg for DON and 31, 47 and 36 μg/kg for HT-2 in wheat, breakfast cereal and maize-based baby food, respectively. Both intra-assay and inter-assay precision were calculated using fortified DON and HT-2 samples. Durum wheat, wheatbased breakfast cereal and maize-based baby food were spiked at various concentration levels and the coefficients of variation calculated ranged from 1.1% to 9.9% for DON and from 1.4% to 11.3% for HT-2. A high correlation was observed between the screening assay and confirmatory mass spectrometry.

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Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽

10.3390/bios8040102 ◽

2018 ◽

Vol 8 (4) ◽

pp. 102 ◽

Cited By ~ 8

Author(s):

Richard Schasfoort ◽

Fikri Abali ◽

Ivan Stojanovic ◽

Gestur Vidarsson ◽

Leon Terstappen

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Mammalian Cells ◽

Living Cells ◽

Label Free ◽

Intact Cells ◽

Cell Parameters ◽

Spr Imaging ◽

Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

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