Breeding Rice Cultivars with Durable Blast Resistance in Colombia

2009 ◽  
pp. 375-383 ◽  
Author(s):  
Fernando Correa Victoria ◽  
Cesar Martinez
Keyword(s):  
Blast Resistance ◽  
Rice Cultivars

scholarly journals Evaluation of indigenous aromatic rice cultivars from sub-Himalayan Terai region of India for nutritional attributes and blast resistance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Debayan Mondal ◽  
Prudveesh Kantamraju ◽  
Susmita Jha ◽  
Gadge Sushant Sundarrao ◽  
Arpan Bhowmik ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Amylose Content ◽  
Blast Resistance ◽  
Starch Content ◽  
Low Cost ◽  
Principal Component ◽  
Blast Disease ◽  
Basmati Rice ◽  
Phenotypic Traits ◽  
Rice Cultivars

AbstractIndigenous folk rice cultivars often possess remarkable but unrevealed potential in terms of nutritional attributes and biotic stress tolerance. The unique cooking qualities and blissful aroma of many of these landraces make it an attractive low-cost alternative to high priced Basmati rice. Sub-Himalayan Terai region is bestowed with great agrobiodiversity in traditional heirloom rice cultivars. In the present study, ninety-nine folk rice cultivars from these regions were collected, purified and characterized for morphological and yield traits. Based on traditional importance and presence of aroma, thirty-five genotypes were selected and analyzed for genetic diversity using micro-satellite marker system. The genotypes were found to be genetically distinct and of high nutritive value. The resistant starch content, amylose content, glycemic index and antioxidant potential of these genotypes represented wide variability and ‘Kataribhog’, ‘Sadanunia’, ‘Chakhao’ etc. were identified as promising genotypes in terms of different nutritional attributes. These cultivars were screened further for resistance against blast disease in field trials and cultivars like ‘Sadanunia’, ‘T4M-3-5’, ‘Chakhao Sampark’ were found to be highly resistant to the blast disease whereas ‘Kalonunia’, ‘Gobindabhog’, ‘Konkanijoha’ were found to be highly susceptible. Principal Component analysis divided the genotypes in distinct groups for nutritional potential and blast tolerance. The resistant and susceptible genotypes were screened for the presence of the blast resistant pi genes and association analysis was performed with disease tolerance. Finally, a logistic model based on phenotypic traits for prediction of the blast susceptibility of the genotypes is proposed with more than 80% accuracy.

scholarly journals Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa

2017 ◽  
Vol 107 (9) ◽  
pp. 1039-1046 ◽  
Author(s):  
Emmanuel M. Mgonja ◽  
Chan Ho Park ◽  
Houxiang Kang ◽  
Elias G. Balimponya ◽  
Stephen Opiyo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Association Mapping ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Blast Resistance ◽  
Genotyping By Sequencing ◽  
Rice Breeding ◽  
Rice Cultivars ◽  
R Genes ◽  
African Rice

Understanding the genetic diversity of rice germplasm is important for the sustainable use of genetic materials in rice breeding and production. Africa is rich in rice genetic resources that can be utilized to boost rice productivity on the continent. A major constraint to rice production in Africa is rice blast, caused by the hemibiotrophic fungal pathogen Magnaporthe oryzae. In this report, we present the results of a genotyping-by-sequencing (GBS)-based diversity analysis of 190 African rice cultivars and an association mapping of blast resistance (R) genes and quantitative trait loci (QTLs). The 190 African cultivars were clustered into three groups based on the 184K single nucleotide polymorphisms generated by GBS. We inoculated the rice cultivars with six African M. oryzae isolates. Association mapping identified 25 genomic regions associated with blast resistance (RABRs) in the rice genome. Moreover, PCR analysis indicated that RABR_23 is associated with the Pi-ta gene on chromosome 12. Our study demonstrates that the combination of GBS-based genetic diversity population analysis and association mapping is effective in identifying rice blast R genes/QTLs that contribute to resistance against African populations of M. oryzae. The identified markers linked to the RABRs and 14 highly resistant cultivars in this study will be useful for rice breeding in Africa.

scholarly journals Bayogenin 3-O-Cellobioside is a novel non-cultivar specific anti-blast metabolite produced in rice in response to Pyricularia oryzae infection

2019 ◽  
Author(s):  
Justice Norvienyeku ◽  
Lili Lin ◽  
Abdul Waheed ◽  
Xiaomin Chen ◽  
Jiandong Bao ◽  
...  
Keyword(s):  
Control Strategy ◽  
Host Resistance ◽  
Blast Resistance ◽  
Differential Resistance ◽  
Host Immunity ◽  
Cereal Crops ◽  
Rice Cultivars ◽  
First Time ◽  
Insight Into

AbstractRice cultivars from japonica and indica lineage possess differential resistance against blast fungus on an account genetic divergence. Whether different rice cultivars also show distinct metabolomic changes in response to P. oryzae, and their role in host resistance, are poorly understood. Here, we examine the responses of six different rice cultivars from japonica and indica lineage challenged with P. oryzae. Both susceptible and resistant rice cultivars expressed several metabolites exclusively during P. oryzae infection, including the saponin Bayogenin 3-O-cellobioside. Bayogenin 3-O-cellobioside level in infected rice directly correlated with their resistant attributes. These findings reveal, for the first time to our knowledge that besides oat, other grass plants including rice produces protective saponins. Our study provides insight into the role of pathogen-mediated metabolomics-reprogramming in host immunity. The correlation between Bayogenin 3-O-Cellobioside levels and blast resistance suggests that engineering saponin expression in cereal crops represents an attractive and sustainable disease control strategy.

scholarly journals In silico modelling and characterization of eight blast resistance proteins in resistant and susceptible rice cultivars

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
R. Chandrakanth ◽  
L. Sunil ◽  
L. Sadashivaiah ◽  
N. S. Devaki
Keyword(s):  
Plant Defense ◽  
Resistance Genes ◽  
In Silico ◽  
Defense Mechanism ◽  
Blast Resistance ◽  
Rice Cultivars ◽  
Resistance Proteins ◽  
In Silico Modeling ◽  
Plant Defense Mechanism ◽  
And Function

Abstract Background Nucleotide-binding site-leucine-rich repeat (NBS-LRR) resistance genes are the largest class of plant resistance genes which play an important role in the plant defense response. These genes are better conserved than others and function as a recognition-based immune system in plants through their encoded proteins. Results Here, we report the effect of Magnaporthe oryzae, the rice blast pathogen inoculation in resistant BR2655 and susceptible HR12 rice cultivars. Transcriptomic profiling was carried out to analyze differential gene expression in these two cultivars. A total of eight NBS-LRR uncharacterized resistance proteins (RP1, RP2, RP3, RP4, RP5, RP6, RP7, and RP8) were selected in these two cultivars for in silico modeling. Modeller 9.22 and SWISS-MODEL servers were used for the homology modeling of eight RPs. ProFunc server was utilized for the prediction of secondary structure and function. The CDvist Web server and Interpro scan server detected the motif and domains in eight RPs. Ramachandran plot of eight RPs confirmed that the modeled structures occupied favorable positions. Conclusions From the present study, computational analysis of these eight RPs may afford insights into their role, function, and valuable resource for studying the intricate details of the plant defense mechanism. Furthermore, the identification of resistance proteins is useful for the development of molecular markers linked to resistance genes.

scholarly journals Yield and yield component performances of local pigmented upland rice cultivars from East Nusa Tenggara, Indonesia in three locations

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Antonius Ndiwa ◽  
Yosep S. Mau
Keyword(s):  
Drought Tolerance ◽  
Grain Yield ◽  
Upland Rice ◽  
Blast Resistance ◽  
Yield Component ◽  
Agronomic Performance ◽  
Rice Cultivars ◽  
Yield Performance ◽  
Rice Genotype

Abstract. Ndiwa ASS, Mau YS. 2019. Yield and yield component performances of local pigmented upland rice cultivars from East Nusa Tenggara, Indonesia in three locations. Trop Drylands 3: 49-55. Several pigmented upland rice cultivars from East Nusa Tenggara Province have been selected based on their agronomic performance, blast resistance, and drought tolerance traits. This study aimed to further elucidate the selected upland rice cultivars for their yield performance and stability in a multi-location trial in three locations. The results showed the significant effect of rice genotype, location, and interactions between rice genotype and location (GxE) on most observed variables, both in each location and across the three locations. Five genotypes produced average grain yield of ≥ 4.0 t/ha over three locations, i.e., NGR-22, PMK-01, ADN-05, Inpago 7 and Aek Sibundong. These five genotypes were found to produce high and stable grain yield under the three growing environments.

scholarly journals Development of blast resistant rice plants using CRISPR / Cas9 system for genome editing

2021 ◽  
Author(s):  
◽  
Fabiano Touzdjian Pinheiro Kohlrausch Távora
Keyword(s):  
Rice Blast ◽  
Blast Resistance ◽  
Rice Variety ◽  
Susceptibility Genes ◽  
Plant Diseases ◽  
Blast Disease ◽  
World Population ◽  
Japonica Rice ◽  
Rice Cultivars ◽  
Short Period

Rice (Oryza sativa L.) is the main food crop for more than half of the world population but unfortunately, it is severely affected by blast, one of the most widespread and devastating plant diseases, caused by the fungus Magnaporthe oryzae. Hence, the development of rice cultivars with greater resistance to blast is one of the main focuses of breeding programs. However, due to the complex biology of the pathogen, rice cultivars genetically resistant to the fungus become susceptible in a short period of time. In this context, the knockout of rice susceptibility genes represents a flourishing approach to obtain rice cultivars with a broader and longer-lasting resistance to M. oryzae. The present study aimed to use the genomic editing technology - CRISPR/Cas9 system, for knocking-out genes engaged with rice susceptibility to fungal infection. From previous transcriptomics results of two semi-isogenic rice lines - NILs infected by M. oryzae, potential rice-blast susceptibility genes were selected. The prospection of candidate genes for gene editing was complemented by a comparative shotgun proteomic analysis of the protein profile of the interaction between IRBLi-F5 (susceptible) and IRBL5-M (resistant) NILs in early stages of M. oryzae infection, that revealed a specific set of proteins potentially associated with susceptibility. After the characterization and validation of gene expression by RT-qPCR of the most prominent candidates, the target genes OsDjA2, OsERF104 and OsPyl5 were selected and submitted to a functional validation via gene silencing in planta, using antisense oligonucleotides (ASO), in which a clear reduction of leaf symptoms was observed in the compatible identification. Subsequently, the model japonica rice variety Nipponbare was transformed with simplex CRISPR/Cas9 vectors aiming to the independent knockout of each target gene. The T1 progeny of rice-edited plants, homozygous for the null (loss of function)-mutation were tested for blast resistance. As expected, mutant plants showed a decrease of disease symptoms in comparison with control lines (transformant non-edited plants). The results obtained in this study can contribute for the development of rice cultivars resistant to blast disease, besides shedding light on new potential rice-blast susceptibility genes.

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