Effect of Temperature on Biofiltration of Nitric Oxide

Author(s):  
K. Thomas Klasson ◽  
Brian H. Davison
1995 ◽  
Vol 2 (4) ◽  
pp. 186-195
Author(s):  
Issei IWAMOTO ◽  
Hisao MORITA ◽  
Atsushi KOSAKA ◽  
Hitoshi TANIHARA ◽  
Kazuyoshi MITA ◽  
...  

1997 ◽  
Vol 43 (4) ◽  
pp. 657-662 ◽  
Author(s):  
Fan Yang ◽  
Eric Troncy ◽  
Martin Francœur ◽  
Bernard Vinet ◽  
Patrick Vinay ◽  
...  

Abstract To measure the concentration of nitrites and nitrates by chemiluminescence, we examined the efficiency of five reducing agents [V(III), Mo(VI) + Fe(II), NaI, Ti(III), and Cr(III)] to reduce nitrite (NO2−) and (or) nitrate (NO3−) to nitric oxide (NO). The effect of each reducing agent on the conversion of different amounts of NO2− and (or) NO3−(100–500 pmol, representing concentrations of 0.4 to 2 μmolar) to NO was determined at 20 °C for NO2− and at 80 °C for NO3−. The effect of temperature from 20 to 90 °C on the conversion of a fixed amount of NO2− or NO3− (400 pmol or 1.6 μmolar) to NO was also determined. These five reducing agents are similarly efficient for the conversion of NO2− to NO at 20 °C. V(III) and Mo(VI) + Fe(II) can completely reduce NO3− to NO at 80 °C. NaI and Cr(III) were unable to convert NO3− to NO. Increased temperature facilitated the conversion of NO3− to NO, rather than that of NO2− to NO. We evaluated the recovery of NO2− and NO3− from plasmas of pig and of dog. Recovery from plasma of both animals was reproducible and near quantitative.


1993 ◽  
Vol 71 (8) ◽  
pp. 629-632 ◽  
Author(s):  
Brian P. Booth ◽  
James F. Brien ◽  
Gerald S. Marks ◽  
Kanji Nakatsu

It has previously been shown that the vasodilatory response to glyceryl trinitrate (GTN) was decreased during hypothermic cardiopulmonary bypass. The purpose of these experiments was to determine the effect of temperature on GTN-induced relaxation and on GTN biotransformation in rabbit aorta. It was determined that the EC50 of GTN on rabbit aortic rings (RARs) was increased significantly from 1.8 × 10−8 M at 37 °C to 3.4 × 10−8 M at 27 °C (p < 0.05). The production of NO by rabbit aortic strips (RASs) was significantly less at 27 °C compared with 37 °C after 80 min, being 9.62 × 10−11 ± 13.2 × 10−11 mol NO/g wet wt. RASs compared with 5.71 × 10−10 ± 9.43 × 10−11 mol NO/g wet wt. RASs, respectively (p < 0.05), after 80 min incubation. There was no difference in the amount of glyceryl-1,2-dinitrate (1,2-GDN) produced from GTN at the two temperatures. The ED20 for NO-induced relaxation of RARs increased from 3.46 × 10−10 ± 2.24 × 10−10 mol at 37 °C to 1.01 × 10−9 ± 4.51 × 10−10 mol at 27 °C (p < 0.05). These data indicate that the biotransformation of GTN and the release of NO were impaired by hypothermia, and that this, as well as a decrease in the tissue response to NO at 27 °C, explains the decrease in GTN activity at reduced body temperatures.Key words: glyceryl trinitrate, nitric oxide, temperature, rabbit aorta, vasodilation.


Nitric Oxide ◽  
1999 ◽  
Vol 3 (5) ◽  
pp. 375-382 ◽  
Author(s):  
Giorgio Venturini ◽  
Marco Colasanti ◽  
Emanuela Fioravanti ◽  
Andrea Bianchini ◽  
Paolo Ascenzi

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