Cell Aggregation Assays

2013 ◽  
pp. 77-92 ◽  
Author(s):  
Delphine Debruyne ◽  
Tom Boterberg ◽  
Marc E. Bracke
Keyword(s):  
Cell Aggregation

scholarly journals Characterization of a novel pyruvylated carbohydrate unit implicated in the cell aggregation of the marine sponge Microciona prolifera

1993 ◽  
Vol 268 (18) ◽  
pp. 13378-13387
Author(s):  
D. Spillmann ◽  
K. Hård ◽  
J. Thomas-Oates ◽  
J.F. Vliegenthart ◽  
G. Misevic ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Cell Aggregation ◽  
Carbohydrate Unit

scholarly journals Sodium Salicylate Influences the Pseudomonas aeruginosa Biofilm Structure and Susceptibility Towards Silver

2021 ◽  
Vol 22 (3) ◽  
pp. 1060
Author(s):  
Erik Gerner ◽  
Sofia Almqvist ◽  
Peter Thomsen ◽  
Maria Werthén ◽  
Margarita Trobos
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Sodium Salicylate ◽  
Treatment Strategies ◽  
Cell Aggregation ◽  
Wound Fluid ◽  
Global Challenge ◽  
3D Collagen ◽  
Biofilm Development ◽  
Biofilm Structure

Hard-to-heal wounds are typically infected with biofilm-producing microorganisms, such as Pseudomonas aeruginosa, which strongly contribute to delayed healing. Due to the global challenge of antimicrobial resistance, alternative treatment strategies are needed. Here, we investigated whether inhibition of quorum sensing (QS) by sodium salicylate in different P. aeruginosa strains (QS-competent, QS-mutant, and chronic wound strains) influences biofilm formation and tolerance to silver. Biofilm formation was evaluated in simulated serum-containing wound fluid in the presence or absence of sodium salicylate (NaSa). Biofilms were established using a 3D collagen-based biofilm model, collagen coated glass, and the Calgary biofilm device. Furthermore, the susceptibility of 48-h-old biofilms formed by laboratory and clinical strains in the presence or absence of NaSa towards silver was evaluated by assessing cell viability. Biofilms formed in the presence of NaSa were more susceptible to silver and contained reduced levels of virulence factors associated with biofilm development than those formed in the absence of NaSa. Biofilm aggregates formed by the wild-type but not the QS mutant strain, were smaller and less heterogenous in size when grown in cultures with NaSa compared to control. These data suggest that NaSa, via a reduction of cell aggregation in biofilms, allows the antiseptic to become more readily available to cells.

Dynamics of cell aggregation during in vitro neurogenesis by immortalized neuroectodermal progenitors

2000 ◽  
Vol 60 (2) ◽  
pp. 184-194 ◽  
Author(s):  
Katalin Schlett ◽  
Andr�s Czir�k ◽  
Kriszti�n T�rnok ◽  
Tam�s Vicsek ◽  
Em�lia Madar�sz
Keyword(s):  
Cell Aggregation

Inhibition of Cell Aggregation by Antibodies directed against Actomyosin

Nature ◽  
1970 ◽  
Vol 226 (5242) ◽  
pp. 261-262 ◽  
Author(s):  
BRYN M. JONES ◽  
RICHARD B. KEMP ◽  
UTE GRÖSCHEL-STEWART
Keyword(s):  
Cell Aggregation
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